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Antioxidant responses in gills and digestive gland of oyster Crassostrea madrasensis (Preston) under lead exposure.
Ecotoxicol Environ Saf. 2017 Aug; 142:87-94.EE

Abstract

Crassostrea are ecologically and economically important bivalves and provide a good livelihood for coastal regions of many countries, including India. This study aims at evaluating the response of the antioxidant defense system in oyster Crassostrea madrasensis against lead (Pb) exposure under laboratory conditions. Antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), and glutathione-s-transferase (GST) and oxidative damage parameter lipid peroxidation (LPO) were measured in the gills and digestive glands of oysters exposed to 1-50µg/l of Pb (NO3)2 over a period of 8 days. LPO index increased progressively with increase in Pb concentration (1, 10, 25 and 50µg/l) in both tissues, gills and digestive gland. Although CAT and SOD activities induced together in the initial phase (upto 6th day), their activities decreased at a later stage of the experiment. However, GST activity increased on 8th day in both the tissues at concentration 10, 25 and 50µg/l indicates the compensatory defense mechanism against oxidative stress. The induced antioxidant responses recorded at 25 and 50µg/l of Pb concentrations suggest the presence of Pb-induced oxidative stress at these concentrations. The results of this work also indicate that LPO, SOD, and GST could be used as biomarkers to assess the impact of Pb on the C. madrasensis. This study concludes that any high level of dissolved Pb concentration (>10µg/l) in surrounding seawater could be harmful to the physiology of the C. madrasensis.

Authors+Show Affiliations

CSIR-National Institute of Oceanography, Dona Paula, Goa 403004, India.CSIR-National Institute of Oceanography, Dona Paula, Goa 403004, India. Electronic address: gmangesh@nio.org.CSIR-National Institute of Oceanography, Dona Paula, Goa 403004, India.CSIR-National Institute of Oceanography, Dona Paula, Goa 403004, India.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28391094

Citation

Shenai-Tirodkar, Prachi S., et al. "Antioxidant Responses in Gills and Digestive Gland of Oyster Crassostrea Madrasensis (Preston) Under Lead Exposure." Ecotoxicology and Environmental Safety, vol. 142, 2017, pp. 87-94.
Shenai-Tirodkar PS, Gauns MU, Mujawar MWA, et al. Antioxidant responses in gills and digestive gland of oyster Crassostrea madrasensis (Preston) under lead exposure. Ecotoxicol Environ Saf. 2017;142:87-94.
Shenai-Tirodkar, P. S., Gauns, M. U., Mujawar, M. W. A., & Ansari, Z. A. (2017). Antioxidant responses in gills and digestive gland of oyster Crassostrea madrasensis (Preston) under lead exposure. Ecotoxicology and Environmental Safety, 142, 87-94. https://doi.org/10.1016/j.ecoenv.2017.03.056
Shenai-Tirodkar PS, et al. Antioxidant Responses in Gills and Digestive Gland of Oyster Crassostrea Madrasensis (Preston) Under Lead Exposure. Ecotoxicol Environ Saf. 2017;142:87-94. PubMed PMID: 28391094.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant responses in gills and digestive gland of oyster Crassostrea madrasensis (Preston) under lead exposure. AU - Shenai-Tirodkar,Prachi S, AU - Gauns,Mangesh U, AU - Mujawar,Mohammad Wassim A, AU - Ansari,Zakir A, Y1 - 2017/04/06/ PY - 2016/11/18/received PY - 2017/03/01/revised PY - 2017/03/20/accepted PY - 2017/4/10/pubmed PY - 2017/9/16/medline PY - 2017/4/10/entrez KW - Antioxidant enzymes KW - Bioaccumulation KW - Biomarker KW - Crassostrea KW - Lead KW - Oxidative stress SP - 87 EP - 94 JF - Ecotoxicology and environmental safety JO - Ecotoxicol Environ Saf VL - 142 N2 - Crassostrea are ecologically and economically important bivalves and provide a good livelihood for coastal regions of many countries, including India. This study aims at evaluating the response of the antioxidant defense system in oyster Crassostrea madrasensis against lead (Pb) exposure under laboratory conditions. Antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), and glutathione-s-transferase (GST) and oxidative damage parameter lipid peroxidation (LPO) were measured in the gills and digestive glands of oysters exposed to 1-50µg/l of Pb (NO3)2 over a period of 8 days. LPO index increased progressively with increase in Pb concentration (1, 10, 25 and 50µg/l) in both tissues, gills and digestive gland. Although CAT and SOD activities induced together in the initial phase (upto 6th day), their activities decreased at a later stage of the experiment. However, GST activity increased on 8th day in both the tissues at concentration 10, 25 and 50µg/l indicates the compensatory defense mechanism against oxidative stress. The induced antioxidant responses recorded at 25 and 50µg/l of Pb concentrations suggest the presence of Pb-induced oxidative stress at these concentrations. The results of this work also indicate that LPO, SOD, and GST could be used as biomarkers to assess the impact of Pb on the C. madrasensis. This study concludes that any high level of dissolved Pb concentration (>10µg/l) in surrounding seawater could be harmful to the physiology of the C. madrasensis. SN - 1090-2414 UR - https://www.unboundmedicine.com/medline/citation/28391094/Antioxidant_responses_in_gills_and_digestive_gland_of_oyster_Crassostrea_madrasensis__Preston__under_lead_exposure_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0147-6513(17)30194-X DB - PRIME DP - Unbound Medicine ER -