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Molecular Characterization and Variation of the Celiac Disease Epitope Domains among α-Gliadin Genes in Aegilops tauschii.
J Agric Food Chem. 2017 Apr 26; 65(16):3422-3429.JA

Abstract

To explore the distribution and quantity of toxic epitopes in α-gliadins from Aegilops tauschii, a total of 133 complete α-gliadin coding sequences were obtained, including 69 pseudogenes with at least one premature stop codon and 64 genes with complete open reading frames (ORFs). Plenty of deletions and single amino acid substitutions were found in the 4 celiac disease (CD) toxic epitope domains through multiple alignments, in which the sequence of DQ2.5-glia-α2 demonstrated the most significant changes. Interestingly, 7 of the 59 α-gliadins were free of any kind of intact CD toxic epitopes, providing potential gene resources for low CD toxicity breeding of common wheat. Analysis of the neighbor-joining tree demonstrates that 2 of the totally 7 α-gliadins cluster within the homologues of Triticum (A genome), and the other 5 group with those of Aegilops Sitopsis (B genome). This result implies that the 7 α-gliadin genes may be originated from the ancestor species of Ae. tauschii, evolved by the homoploid hybrid of Triticum and Aegilops Sitopsis. The remaining 52 α-gliadins form a separate clade from other homologues of A and B genomes, suggesting a recent rapid gene expansion by gene duplication associated with the species adaptation.

Authors+Show Affiliations

School of Life Science, Henan University , Kaifeng, 475004, Henan, People's Republic of China. Institute of Plant Stress Biology, Henan University , Kaifeng, 475004, People's Republic of China.School of Life Science, Henan University , Kaifeng, 475004, Henan, People's Republic of China. Institute of Plant Stress Biology, Henan University , Kaifeng, 475004, People's Republic of China.Institute of Plant Stress Biology, Henan University , Kaifeng, 475004, People's Republic of China.School of Life Science, Henan University , Kaifeng, 475004, Henan, People's Republic of China. Institute of Plant Stress Biology, Henan University , Kaifeng, 475004, People's Republic of China.School of Life Science, Henan University , Kaifeng, 475004, Henan, People's Republic of China. Institute of Plant Stress Biology, Henan University , Kaifeng, 475004, People's Republic of China.School of Life Science, Henan University , Kaifeng, 475004, Henan, People's Republic of China.School of Life Science, Henan University , Kaifeng, 475004, Henan, People's Republic of China.School of Life Science, Henan University , Kaifeng, 475004, Henan, People's Republic of China. Institute of Plant Stress Biology, Henan University , Kaifeng, 475004, People's Republic of China.School of Life Science, Henan University , Kaifeng, 475004, Henan, People's Republic of China. Institute of Plant Stress Biology, Henan University , Kaifeng, 475004, People's Republic of China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28391694

Citation

Li, Yu-Ge, et al. "Molecular Characterization and Variation of the Celiac Disease Epitope Domains Among α-Gliadin Genes in Aegilops Tauschii." Journal of Agricultural and Food Chemistry, vol. 65, no. 16, 2017, pp. 3422-3429.
Li YG, Liang HH, Bai SL, et al. Molecular Characterization and Variation of the Celiac Disease Epitope Domains among α-Gliadin Genes in Aegilops tauschii. J Agric Food Chem. 2017;65(16):3422-3429.
Li, Y. G., Liang, H. H., Bai, S. L., Zhou, Y., Sun, G., Su, Y. R., Gao, A. L., Zhang, D. L., & Li, S. P. (2017). Molecular Characterization and Variation of the Celiac Disease Epitope Domains among α-Gliadin Genes in Aegilops tauschii. Journal of Agricultural and Food Chemistry, 65(16), 3422-3429. https://doi.org/10.1021/acs.jafc.7b00338
Li YG, et al. Molecular Characterization and Variation of the Celiac Disease Epitope Domains Among α-Gliadin Genes in Aegilops Tauschii. J Agric Food Chem. 2017 Apr 26;65(16):3422-3429. PubMed PMID: 28391694.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular Characterization and Variation of the Celiac Disease Epitope Domains among α-Gliadin Genes in Aegilops tauschii. AU - Li,Yu-Ge, AU - Liang,Hui-Hui, AU - Bai,Sheng-Long, AU - Zhou,Yun, AU - Sun,Guiling, AU - Su,Ya-Rui, AU - Gao,An-Li, AU - Zhang,Da-Le, AU - Li,Suo-Ping, Y1 - 2017/04/18/ PY - 2017/4/11/pubmed PY - 2019/3/21/medline PY - 2017/4/11/entrez KW - Aegilops tauschii KW - celiac disease KW - phylogenetic relationships KW - toxic epitope KW - α-gliadin SP - 3422 EP - 3429 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 65 IS - 16 N2 - To explore the distribution and quantity of toxic epitopes in α-gliadins from Aegilops tauschii, a total of 133 complete α-gliadin coding sequences were obtained, including 69 pseudogenes with at least one premature stop codon and 64 genes with complete open reading frames (ORFs). Plenty of deletions and single amino acid substitutions were found in the 4 celiac disease (CD) toxic epitope domains through multiple alignments, in which the sequence of DQ2.5-glia-α2 demonstrated the most significant changes. Interestingly, 7 of the 59 α-gliadins were free of any kind of intact CD toxic epitopes, providing potential gene resources for low CD toxicity breeding of common wheat. Analysis of the neighbor-joining tree demonstrates that 2 of the totally 7 α-gliadins cluster within the homologues of Triticum (A genome), and the other 5 group with those of Aegilops Sitopsis (B genome). This result implies that the 7 α-gliadin genes may be originated from the ancestor species of Ae. tauschii, evolved by the homoploid hybrid of Triticum and Aegilops Sitopsis. The remaining 52 α-gliadins form a separate clade from other homologues of A and B genomes, suggesting a recent rapid gene expansion by gene duplication associated with the species adaptation. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/28391694/Molecular_Characterization_and_Variation_of_the_Celiac_Disease_Epitope_Domains_among_α_Gliadin_Genes_in_Aegilops_tauschii_ L2 - https://doi.org/10.1021/acs.jafc.7b00338 DB - PRIME DP - Unbound Medicine ER -