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Gold nanoparticles modified porous silicon chip for SALDI-MS determination of glutathione in cells.
Talanta. 2017 Jun 01; 168:222-229.T

Abstract

The gold nanoparticles (Au NPs) modified porous silicon chip based surface assisted laser desorption/ionization mass spectrometry (SALDI-MS) was developed to capture and analyze glutathione (GSH) in cells. With silver-assisted chemical etching, Ag nanoparticles (Ag NPs) were generated and deposited on the silicon surface and the nanopores were etched on silicon substrate. Then Au NPs were in-situ synthesized on the ridges of silicon nanopores. This Ag-Au NPs modified porous silicon surface could specially capture and enrich thiol compounds through Au-S binding, and it could also function as matrix to assist ionization for SALDI-MS. The silicon chip was array patterned for high throughput SALDI-MS detection. GSH and cysteine could be distinguished without the interference from matrix signals. This approach was successfully applied to preconcentration and detection of GSH in Caco-2 cells. The GSH alterations in cells under drug stimulation were investigated. This invented silicon chip showed great potential for more efficient analysis of small thiol biomarkers in complex biological samples.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Wang J
Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Tsinghua University, Beijing 100084, China; Graduate School of Shenzhen, Tsinghua University, Shenzhen 518055, China.
Jie M
Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Tsinghua University, Beijing 100084, China.
Li H
Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Tsinghua University, Beijing 100084, China. Electronic address: lihaifang@mail.tsinghua.edu.cn.
Lin L
Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Tsinghua University, Beijing 100084, China.
He Z
Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Tsinghua University, Beijing 100084, China.
Wang S
Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Tsinghua University, Beijing 100084, China.
Lin JM
Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Tsinghua University, Beijing 100084, China. Electronic address: jmlin@mail.tsinghua.edu.cn.

MeSH

Caco-2 CellsGlutathioneGoldHumansMetal NanoparticlesNanoporesPorositySiliconSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28391846

Citation

Wang, Jing, et al. "Gold Nanoparticles Modified Porous Silicon Chip for SALDI-MS Determination of Glutathione in Cells." Talanta, vol. 168, 2017, pp. 222-229.
Wang J, Jie M, Li H, et al. Gold nanoparticles modified porous silicon chip for SALDI-MS determination of glutathione in cells. Talanta. 2017;168:222-229.
Wang, J., Jie, M., Li, H., Lin, L., He, Z., Wang, S., & Lin, J. M. (2017). Gold nanoparticles modified porous silicon chip for SALDI-MS determination of glutathione in cells. Talanta, 168, 222-229. https://doi.org/10.1016/j.talanta.2017.02.041
Wang J, et al. Gold Nanoparticles Modified Porous Silicon Chip for SALDI-MS Determination of Glutathione in Cells. Talanta. 2017 Jun 1;168:222-229. PubMed PMID: 28391846.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gold nanoparticles modified porous silicon chip for SALDI-MS determination of glutathione in cells. AU - Wang,Jing, AU - Jie,Mingsha, AU - Li,Haifang, AU - Lin,Luyao, AU - He,Ziyi, AU - Wang,Shiqi, AU - Lin,Jin-Ming, Y1 - 2017/02/21/ PY - 2016/08/31/received PY - 2017/02/12/revised PY - 2017/02/19/accepted PY - 2017/4/11/entrez PY - 2017/4/11/pubmed PY - 2018/9/15/medline KW - Cells KW - Glutathione KW - Gold nanoparticles KW - Laser desorption/ionization mass spectrometry KW - Porous silicon SP - 222 EP - 229 JF - Talanta JO - Talanta VL - 168 N2 - The gold nanoparticles (Au NPs) modified porous silicon chip based surface assisted laser desorption/ionization mass spectrometry (SALDI-MS) was developed to capture and analyze glutathione (GSH) in cells. With silver-assisted chemical etching, Ag nanoparticles (Ag NPs) were generated and deposited on the silicon surface and the nanopores were etched on silicon substrate. Then Au NPs were in-situ synthesized on the ridges of silicon nanopores. This Ag-Au NPs modified porous silicon surface could specially capture and enrich thiol compounds through Au-S binding, and it could also function as matrix to assist ionization for SALDI-MS. The silicon chip was array patterned for high throughput SALDI-MS detection. GSH and cysteine could be distinguished without the interference from matrix signals. This approach was successfully applied to preconcentration and detection of GSH in Caco-2 cells. The GSH alterations in cells under drug stimulation were investigated. This invented silicon chip showed great potential for more efficient analysis of small thiol biomarkers in complex biological samples. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/28391846/Gold_nanoparticles_modified_porous_silicon_chip_for_SALDI_MS_determination_of_glutathione_in_cells_ DB - PRIME DP - Unbound Medicine ER -
Grapherence [↓4]

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