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Novel mutations in gyrA and parC among Shigella sonnei strains from Jiangsu Province of China, 2002-2011.
Int J Infect Dis. 2017 Jun; 59:44-49.IJ

Abstract

BACKGROUND

To investigate fluoroquinolone resistance and associated mechanisms of Shigella sonnei isolates in Jiangsu Province of China between 2002 and 2011.

METHODS

All 337 unduplicated S. sonnei isolates were collected from hospitals in Jiangsu Province from January 2002 to December 2011. Fluoroquinolone susceptibility was characterized by Kirby-Bauer disk diffusion method, and direct nucleotide sequencing of genes of the quinolone resistance determining regions were conducted. Also, the transferable quinolone resistance determinants, including qnrA, qnrB, qnrC, qnrD, qnrS, aac-(6')-Ib-cr and qepA were amplified by PCR.

RESULTS

Among 950 Shigella isolates, 337 (35.5%) were identified as S. sonnei, of which 76.6% displayed nalidixic acid resistance and norfloxacin-resistant isolates appeared in 2005-2009, with an average resistance rate of 21.8%. Commonly reported point mutations of Ser83Leu and Asp87Asn/Gly in gyrA and Ser80Ile in parC were detected, with mutation rates of 78.0%, 9.5% and 30.3%, respectively, while no alteration in gyrB or parE were detected. Besides, His211Tyr mutation in gyrA was first reported in a S. sonnei strain in 2009 and two novel mutations in parC were found, of which Met86Trp occurred in another strain in 2009 and Ser129Pro appeared every year except 2011 (28.8%). Plasmid-mediated quinolone resistance determinants were found in 23 isolates and 19 of these isolates were resistant to both nalidixic acid and norfloxacin. qnrB, qnrS, aac-(6')-Ib-cr and qepA were detected in 1, 7, 14 and 2 S. sonnei strains, relatively, and the most abundant PMQR gene found in this work was aac-(6')-Ib-cr (4.2%).

INTERPRETATION & CONCLUSIONS

S. sonnei became increasingly important as fluoroquinolone-resistant isolates emerged, and further detection on the resistant genes would be useful in the treatment and control of this infection.

Authors+Show Affiliations

Medical Technology School, Xuzhou Medical University, Xuzhou 221004, China; Department of Laboratory Medicine, Affiliated Hospital of Xuzhou Medical University, Xuzhou 221002, China.Medical Technology School, Xuzhou Medical University, Xuzhou 221004, China.Medical Technology School, Xuzhou Medical University, Xuzhou 221004, China.Medical Technology School, Xuzhou Medical University, Xuzhou 221004, China.Medical Technology School, Xuzhou Medical University, Xuzhou 221004, China.Medical Technology School, Xuzhou Medical University, Xuzhou 221004, China.Medical Technology School, Xuzhou Medical University, Xuzhou 221004, China; Department of Laboratory Medicine, Affiliated Hospital of Xuzhou Medical University, Xuzhou 221002, China. Electronic address: pingm62@aliyun.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28392317

Citation

Gu, Bing, et al. "Novel Mutations in gyrA and parC Among Shigella Sonnei Strains From Jiangsu Province of China, 2002-2011." International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases, vol. 59, 2017, pp. 44-49.
Gu B, Qin TT, Fan WT, et al. Novel mutations in gyrA and parC among Shigella sonnei strains from Jiangsu Province of China, 2002-2011. Int J Infect Dis. 2017;59:44-49.
Gu, B., Qin, T. T., Fan, W. T., Bi, R. R., Chen, Y., Li, Y., & Ma, P. (2017). Novel mutations in gyrA and parC among Shigella sonnei strains from Jiangsu Province of China, 2002-2011. International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases, 59, 44-49. https://doi.org/10.1016/j.ijid.2017.03.023
Gu B, et al. Novel Mutations in gyrA and parC Among Shigella Sonnei Strains From Jiangsu Province of China, 2002-2011. Int J Infect Dis. 2017;59:44-49. PubMed PMID: 28392317.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Novel mutations in gyrA and parC among Shigella sonnei strains from Jiangsu Province of China, 2002-2011. AU - Gu,Bing, AU - Qin,Ting-Ting, AU - Fan,Wen-Ting, AU - Bi,Ru-Ru, AU - Chen,Ying, AU - Li,Ying, AU - Ma,Ping, Y1 - 2017/04/07/ PY - 2016/12/08/received PY - 2017/03/21/revised PY - 2017/03/31/accepted PY - 2017/4/11/pubmed PY - 2017/10/28/medline PY - 2017/4/11/entrez KW - China KW - QRDR KW - Shigella sonnei KW - aac-(6′)-Ib-cr KW - fluoroquinolone resistant SP - 44 EP - 49 JF - International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases JO - Int J Infect Dis VL - 59 N2 - BACKGROUND: To investigate fluoroquinolone resistance and associated mechanisms of Shigella sonnei isolates in Jiangsu Province of China between 2002 and 2011. METHODS: All 337 unduplicated S. sonnei isolates were collected from hospitals in Jiangsu Province from January 2002 to December 2011. Fluoroquinolone susceptibility was characterized by Kirby-Bauer disk diffusion method, and direct nucleotide sequencing of genes of the quinolone resistance determining regions were conducted. Also, the transferable quinolone resistance determinants, including qnrA, qnrB, qnrC, qnrD, qnrS, aac-(6')-Ib-cr and qepA were amplified by PCR. RESULTS: Among 950 Shigella isolates, 337 (35.5%) were identified as S. sonnei, of which 76.6% displayed nalidixic acid resistance and norfloxacin-resistant isolates appeared in 2005-2009, with an average resistance rate of 21.8%. Commonly reported point mutations of Ser83Leu and Asp87Asn/Gly in gyrA and Ser80Ile in parC were detected, with mutation rates of 78.0%, 9.5% and 30.3%, respectively, while no alteration in gyrB or parE were detected. Besides, His211Tyr mutation in gyrA was first reported in a S. sonnei strain in 2009 and two novel mutations in parC were found, of which Met86Trp occurred in another strain in 2009 and Ser129Pro appeared every year except 2011 (28.8%). Plasmid-mediated quinolone resistance determinants were found in 23 isolates and 19 of these isolates were resistant to both nalidixic acid and norfloxacin. qnrB, qnrS, aac-(6')-Ib-cr and qepA were detected in 1, 7, 14 and 2 S. sonnei strains, relatively, and the most abundant PMQR gene found in this work was aac-(6')-Ib-cr (4.2%). INTERPRETATION & CONCLUSIONS: S. sonnei became increasingly important as fluoroquinolone-resistant isolates emerged, and further detection on the resistant genes would be useful in the treatment and control of this infection. SN - 1878-3511 UR - https://www.unboundmedicine.com/medline/citation/28392317/Novel_mutations_in_gyrA_and_parC_among_Shigella_sonnei_strains_from_Jiangsu_Province_of_China_2002_2011_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1201-9712(17)30108-X DB - PRIME DP - Unbound Medicine ER -