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Punicalagin reduces H2O2-induced cytotoxicity and apoptosis in PC12 cells by modulating the levels of reactive oxygen species.
Nutr Neurosci. 2018 Jul; 21(6):447-454.NN

Abstract

BACKGROUND

Oxidative stress has long been linked to neuronal cell death in many neurodegenerative diseases. Antioxidant conventional supplements are poorly effective in preventing neuronal damage caused by oxidative stress due to their inability to cross the blood brain barrier. Hence the use of molecules extracted from plants and fruits such as phenolics, flavonoids, and terpenoids compounds constitute a new wave of antioxidant therapies to defend against free radicals.

OBJECTIVE

In this study we examined the effects of punicalagin, a ellagitannin isolated from the pomegranate juice, on a rat adrenal pheochromocytoma cell line, treated with hydrogen peroxide, evaluating the viability, oxidation potential, mitochondrial function, and eventual apoptosis.

METHODS

This study was performed on PC12 cells pretreated with punicalagin (0.5, 1, 5, 10 e 20 µM) 24 hours before of the damage by hydrogen peroxide (H2O2). H2O2 concentration (300 µM) used in our study was determined by preliminary experiments of time course. The cell viability and ROS production were evaluated by MTS assay and cytofluorometry assays, respectively. Subsequently, the number of apoptotic-positive cells and mitochondrial transmembrane potential, were measured by flow cytometry, in the same experimental paradigm. Finally, the expression of Bax and enzymatic activity of Caspase 3, some of the principle actors of programmed cell death, were investigated by semiquantitative PCR and utilizing a colorimetric assay kit, respectively.

RESULTS

We found that pretreatment with punicalagin protected the cells from H2O2-induced damage. In particular, the protective effect seemed to be correlated with a control both in radical oxygen species production and in mitochondrial functions. In fact the cells treated with H2O2 showed an altered mitochondrial membrane integrity while the pretreatment with punicalagin retained both the cellular viability and the mitochondrial membrane potential similar to the control. Furthermore, the punicalagin, modulated the apoptotic cascade triggered reducing Bax gene expression and Caspase 3 activity.

DISCUSSION

Results of the present study demonstrated a neuroprotective effect of punicalagin on H2O2-induced PC12 cell death, including mitochondria damage and expression of apoptotic gene Bax; therefore we hypothesize a possible prevent role for this molecule in neurodegenerative diseases related to oxidative stress.

Authors+Show Affiliations

a CNR-ICRM Istituto di"Chimica del Riconoscimento Molecolare", c/o Istituto di Biochimica e Biochimica Clinica , Università Cattolica del Sacro Cuore , Rome , Italy.b Istituto di Patologia Generale , Università Cattolica del Sacro Cuore , Rome , Italy.a CNR-ICRM Istituto di"Chimica del Riconoscimento Molecolare", c/o Istituto di Biochimica e Biochimica Clinica , Università Cattolica del Sacro Cuore , Rome , Italy.c Istituto di Farmacologia , Università Cattolica del Sacro Cuore , Rome , Italy.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28393656

Citation

Clementi, Maria Elisabetta, et al. "Punicalagin Reduces H2O2-induced Cytotoxicity and Apoptosis in PC12 Cells By Modulating the Levels of Reactive Oxygen Species." Nutritional Neuroscience, vol. 21, no. 6, 2018, pp. 447-454.
Clementi ME, Pani G, Sampaolese B, et al. Punicalagin reduces H2O2-induced cytotoxicity and apoptosis in PC12 cells by modulating the levels of reactive oxygen species. Nutr Neurosci. 2018;21(6):447-454.
Clementi, M. E., Pani, G., Sampaolese, B., & Tringali, G. (2018). Punicalagin reduces H2O2-induced cytotoxicity and apoptosis in PC12 cells by modulating the levels of reactive oxygen species. Nutritional Neuroscience, 21(6), 447-454. https://doi.org/10.1080/1028415X.2017.1306935
Clementi ME, et al. Punicalagin Reduces H2O2-induced Cytotoxicity and Apoptosis in PC12 Cells By Modulating the Levels of Reactive Oxygen Species. Nutr Neurosci. 2018;21(6):447-454. PubMed PMID: 28393656.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Punicalagin reduces H2O2-induced cytotoxicity and apoptosis in PC12 cells by modulating the levels of reactive oxygen species. AU - Clementi,Maria Elisabetta, AU - Pani,Giovambattista, AU - Sampaolese,Beatrice, AU - Tringali,Giuseppe, Y1 - 2017/04/09/ PY - 2017/4/11/pubmed PY - 2018/12/26/medline PY - 2017/4/11/entrez KW - Apoptosis KW - Mitochondrial dysfunction KW - Neurodegeneration KW - Oxidative stress KW - Punicalagin SP - 447 EP - 454 JF - Nutritional neuroscience JO - Nutr Neurosci VL - 21 IS - 6 N2 - BACKGROUND: Oxidative stress has long been linked to neuronal cell death in many neurodegenerative diseases. Antioxidant conventional supplements are poorly effective in preventing neuronal damage caused by oxidative stress due to their inability to cross the blood brain barrier. Hence the use of molecules extracted from plants and fruits such as phenolics, flavonoids, and terpenoids compounds constitute a new wave of antioxidant therapies to defend against free radicals. OBJECTIVE: In this study we examined the effects of punicalagin, a ellagitannin isolated from the pomegranate juice, on a rat adrenal pheochromocytoma cell line, treated with hydrogen peroxide, evaluating the viability, oxidation potential, mitochondrial function, and eventual apoptosis. METHODS: This study was performed on PC12 cells pretreated with punicalagin (0.5, 1, 5, 10 e 20 µM) 24 hours before of the damage by hydrogen peroxide (H2O2). H2O2 concentration (300 µM) used in our study was determined by preliminary experiments of time course. The cell viability and ROS production were evaluated by MTS assay and cytofluorometry assays, respectively. Subsequently, the number of apoptotic-positive cells and mitochondrial transmembrane potential, were measured by flow cytometry, in the same experimental paradigm. Finally, the expression of Bax and enzymatic activity of Caspase 3, some of the principle actors of programmed cell death, were investigated by semiquantitative PCR and utilizing a colorimetric assay kit, respectively. RESULTS: We found that pretreatment with punicalagin protected the cells from H2O2-induced damage. In particular, the protective effect seemed to be correlated with a control both in radical oxygen species production and in mitochondrial functions. In fact the cells treated with H2O2 showed an altered mitochondrial membrane integrity while the pretreatment with punicalagin retained both the cellular viability and the mitochondrial membrane potential similar to the control. Furthermore, the punicalagin, modulated the apoptotic cascade triggered reducing Bax gene expression and Caspase 3 activity. DISCUSSION: Results of the present study demonstrated a neuroprotective effect of punicalagin on H2O2-induced PC12 cell death, including mitochondria damage and expression of apoptotic gene Bax; therefore we hypothesize a possible prevent role for this molecule in neurodegenerative diseases related to oxidative stress. SN - 1476-8305 UR - https://www.unboundmedicine.com/medline/citation/28393656/Punicalagin_reduces_H2O2_induced_cytotoxicity_and_apoptosis_in_PC12_cells_by_modulating_the_levels_of_reactive_oxygen_species_ L2 - https://www.tandfonline.com/doi/full/10.1080/1028415X.2017.1306935 DB - PRIME DP - Unbound Medicine ER -