Tags

Type your tag names separated by a space and hit enter

Sevoflurane decreases self-renewal capacity and causes c-Jun N-terminal kinase-mediated damage of rat fetal neural stem cells.
Sci Rep. 2017 04 10; 7:46304.SR

Abstract

Increasing studies have demonstrated that sevoflurane can induce neurotoxicity in the developing brains. JNK normally promotes apoptosis. It was hypothesized that sevoflurane affected the proliferation and differentiation of FNSCs and induced cell apoptosis, which caused the learning and memory deficits via JNK pathway. Sevoflurane at a concentration of 1.2% did not induce damage on the FNSCS. However, concentrations of 2.4% and 4.8% decreased the cell viability, as shown by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and increased apoptosis, as shown by flow cytometry. The 5-ethynyl-2'-deoxyuridine (EdU) incorporation assay demonstrated that 4.8% sevoflurane reduced the proliferation of FNSCs. Compared with the control group, the 4.8% sevoflurane group showed a decrease in the proportion of undifferentiated FNSCs at 6-h exposure; 4.8% sevoflurane could increase the p-JNK/JNK ratio. JNK inhibition by the specific inhibitor SP600125 enhanced partially the cell viability. Cumulatively, 4.8% sevoflurane induced significant damage on FNSCs; it decreased cell proliferation and proportion of undifferentiated cells as well. JNK pathway might play a key role in the decrease in survival of FNSCs induced by an inhaled anesthetic. The present findings might raise the possibility that JNK inhibition has therapeutic potential in protecting FNSCs from the adverse effects of the inhaled anesthetic.

Authors+Show Affiliations

Department of Anesthesiology, International Peace Maternity and Child Health Hospital, Shanghai Jiao Tong University School of Medicine, 200030, Shanghai, China.Department of Anesthesiology, The First Affiliated Hospital of Wannan Medical College, Wuhu, 241000, Anhui, China.Department of Anesthesiology, First Affiliated Hospital of AnHui Medical University, Hefei, 230022, Anhui, China.Department of Anesthesiology, International Peace Maternity and Child Health Hospital, Shanghai Jiao Tong University School of Medicine, 200030, Shanghai, China.Department of Anesthesiology, International Peace Maternity and Child Health Hospital, Shanghai Jiao Tong University School of Medicine, 200030, Shanghai, China.Department of Anesthesiology, International Peace Maternity and Child Health Hospital, Shanghai Jiao Tong University School of Medicine, 200030, Shanghai, China.Department of Anesthesiology, First Affiliated Hospital of AnHui Medical University, Hefei, 230022, Anhui, China.Department of Biochemistry, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

28393934

Citation

Yang, Zeyong, et al. "Sevoflurane Decreases Self-renewal Capacity and Causes c-Jun N-terminal Kinase-mediated Damage of Rat Fetal Neural Stem Cells." Scientific Reports, vol. 7, 2017, p. 46304.
Yang Z, Lv J, Li X, et al. Sevoflurane decreases self-renewal capacity and causes c-Jun N-terminal kinase-mediated damage of rat fetal neural stem cells. Sci Rep. 2017;7:46304.
Yang, Z., Lv, J., Li, X., Meng, Q., Yang, Q., Ma, W., Li, Y., & Ke, Z. J. (2017). Sevoflurane decreases self-renewal capacity and causes c-Jun N-terminal kinase-mediated damage of rat fetal neural stem cells. Scientific Reports, 7, 46304. https://doi.org/10.1038/srep46304
Yang Z, et al. Sevoflurane Decreases Self-renewal Capacity and Causes c-Jun N-terminal Kinase-mediated Damage of Rat Fetal Neural Stem Cells. Sci Rep. 2017 04 10;7:46304. PubMed PMID: 28393934.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sevoflurane decreases self-renewal capacity and causes c-Jun N-terminal kinase-mediated damage of rat fetal neural stem cells. AU - Yang,Zeyong, AU - Lv,Jingjing, AU - Li,Xingxing, AU - Meng,Qiong, AU - Yang,Qiling, AU - Ma,Wei, AU - Li,Yuanhai, AU - Ke,Zun Ji, Y1 - 2017/04/10/ PY - 2016/09/21/received PY - 2017/03/14/accepted PY - 2017/4/11/entrez PY - 2017/4/11/pubmed PY - 2018/11/28/medline SP - 46304 EP - 46304 JF - Scientific reports JO - Sci Rep VL - 7 N2 - Increasing studies have demonstrated that sevoflurane can induce neurotoxicity in the developing brains. JNK normally promotes apoptosis. It was hypothesized that sevoflurane affected the proliferation and differentiation of FNSCs and induced cell apoptosis, which caused the learning and memory deficits via JNK pathway. Sevoflurane at a concentration of 1.2% did not induce damage on the FNSCS. However, concentrations of 2.4% and 4.8% decreased the cell viability, as shown by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and increased apoptosis, as shown by flow cytometry. The 5-ethynyl-2'-deoxyuridine (EdU) incorporation assay demonstrated that 4.8% sevoflurane reduced the proliferation of FNSCs. Compared with the control group, the 4.8% sevoflurane group showed a decrease in the proportion of undifferentiated FNSCs at 6-h exposure; 4.8% sevoflurane could increase the p-JNK/JNK ratio. JNK inhibition by the specific inhibitor SP600125 enhanced partially the cell viability. Cumulatively, 4.8% sevoflurane induced significant damage on FNSCs; it decreased cell proliferation and proportion of undifferentiated cells as well. JNK pathway might play a key role in the decrease in survival of FNSCs induced by an inhaled anesthetic. The present findings might raise the possibility that JNK inhibition has therapeutic potential in protecting FNSCs from the adverse effects of the inhaled anesthetic. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/28393934/Sevoflurane_decreases_self_renewal_capacity_and_causes_c_Jun_N_terminal_kinase_mediated_damage_of_rat_fetal_neural_stem_cells_ L2 - http://dx.doi.org/10.1038/srep46304 DB - PRIME DP - Unbound Medicine ER -