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An oil containing EPA and DHA from transgenic Camelina sativa to replace marine fish oil in feeds for Atlantic salmon (Salmo salar L.): Effects on intestinal transcriptome, histology, tissue fatty acid profiles and plasma biochemistry.
PLoS One. 2017; 12(4):e0175415.Plos

Abstract

New de novo sources of omega 3 (n-3) long chain polyunsaturated fatty acids (LC-PUFA) are required as alternatives to fish oil in aquafeeds in order to maintain adequate levels of the beneficial fatty acids, eicosapentaenoic and docosahexaenoic (EPA and DHA, respectively). The present study investigated the use of an EPA+DHA oil derived from transgenic Camelina sativa in Atlantic salmon (Salmo salar) feeds containing low levels of fishmeal (35%) and fish oil (10%), reflecting current commercial formulations, to determine the impacts on tissue fatty acid profile, intestinal transcriptome, and health of farmed salmon. Post-smolt Atlantic salmon were fed for 12-weeks with one of three experimental diets containing either a blend of fish oil/rapeseed oil (FO), wild-type camelina oil (WCO) or transgenic camelina oil (DCO) as added lipid source. The DCO diet did not affect any of the fish performance or health parameters studied. Analyses of the mid and hindgut transcriptomes showed only mild effects on metabolism. Flesh of fish fed the DCO diet accumulated almost double the amount of n-3 LC-PUFA than fish fed the FO or WCO diets, indicating that these oils from transgenic oilseeds offer the opportunity to increase the n-3 LC-PUFA in farmed fish to levels comparable to those found a decade ago.

Authors+Show Affiliations

Institute of Aquaculture, Faculty of Natural Sciences, University of Stirling, Stirling, United Kingdom.Norwegian University of Science and Technology, Department of Biology, Trondheim, Norway.Institute of Aquaculture, Faculty of Natural Sciences, University of Stirling, Stirling, United Kingdom.Norwegian University of Science and Technology, Department of Biology, Trondheim, Norway.Department of Biological Chemistry and Crop Protection, Rothamsted Research, Harpenden, United Kingdom.Department of Biological Chemistry and Crop Protection, Rothamsted Research, Harpenden, United Kingdom.Department of Biological Chemistry and Crop Protection, Rothamsted Research, Harpenden, United Kingdom.Biomar AS, Trondheim, Norway.Institute of Marine Research, Matre, Matredal, Norway.Department of Biological Chemistry and Crop Protection, Rothamsted Research, Harpenden, United Kingdom.Institute of Aquaculture, Faculty of Natural Sciences, University of Stirling, Stirling, United Kingdom.Norwegian University of Science and Technology, Department of Biology, Trondheim, Norway.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28403232

Citation

Betancor, Mónica B., et al. "An Oil Containing EPA and DHA From Transgenic Camelina Sativa to Replace Marine Fish Oil in Feeds for Atlantic Salmon (Salmo Salar L.): Effects On Intestinal Transcriptome, Histology, Tissue Fatty Acid Profiles and Plasma Biochemistry." PloS One, vol. 12, no. 4, 2017, pp. e0175415.
Betancor MB, Li K, Sprague M, et al. An oil containing EPA and DHA from transgenic Camelina sativa to replace marine fish oil in feeds for Atlantic salmon (Salmo salar L.): Effects on intestinal transcriptome, histology, tissue fatty acid profiles and plasma biochemistry. PLoS ONE. 2017;12(4):e0175415.
Betancor, M. B., Li, K., Sprague, M., Bardal, T., Sayanova, O., Usher, S., Han, L., Måsøval, K., Torrissen, O., Napier, J. A., Tocher, D. R., & Olsen, R. E. (2017). An oil containing EPA and DHA from transgenic Camelina sativa to replace marine fish oil in feeds for Atlantic salmon (Salmo salar L.): Effects on intestinal transcriptome, histology, tissue fatty acid profiles and plasma biochemistry. PloS One, 12(4), e0175415. https://doi.org/10.1371/journal.pone.0175415
Betancor MB, et al. An Oil Containing EPA and DHA From Transgenic Camelina Sativa to Replace Marine Fish Oil in Feeds for Atlantic Salmon (Salmo Salar L.): Effects On Intestinal Transcriptome, Histology, Tissue Fatty Acid Profiles and Plasma Biochemistry. PLoS ONE. 2017;12(4):e0175415. PubMed PMID: 28403232.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An oil containing EPA and DHA from transgenic Camelina sativa to replace marine fish oil in feeds for Atlantic salmon (Salmo salar L.): Effects on intestinal transcriptome, histology, tissue fatty acid profiles and plasma biochemistry. AU - Betancor,Mónica B, AU - Li,Keshuai, AU - Sprague,Matthew, AU - Bardal,Tora, AU - Sayanova,Olga, AU - Usher,Sarah, AU - Han,Lihua, AU - Måsøval,Kjell, AU - Torrissen,Ole, AU - Napier,Johnathan A, AU - Tocher,Douglas R, AU - Olsen,Rolf Erik, Y1 - 2017/04/12/ PY - 2016/11/14/received PY - 2017/03/24/accepted PY - 2017/4/14/entrez PY - 2017/4/14/pubmed PY - 2017/4/22/medline SP - e0175415 EP - e0175415 JF - PloS one JO - PLoS ONE VL - 12 IS - 4 N2 - New de novo sources of omega 3 (n-3) long chain polyunsaturated fatty acids (LC-PUFA) are required as alternatives to fish oil in aquafeeds in order to maintain adequate levels of the beneficial fatty acids, eicosapentaenoic and docosahexaenoic (EPA and DHA, respectively). The present study investigated the use of an EPA+DHA oil derived from transgenic Camelina sativa in Atlantic salmon (Salmo salar) feeds containing low levels of fishmeal (35%) and fish oil (10%), reflecting current commercial formulations, to determine the impacts on tissue fatty acid profile, intestinal transcriptome, and health of farmed salmon. Post-smolt Atlantic salmon were fed for 12-weeks with one of three experimental diets containing either a blend of fish oil/rapeseed oil (FO), wild-type camelina oil (WCO) or transgenic camelina oil (DCO) as added lipid source. The DCO diet did not affect any of the fish performance or health parameters studied. Analyses of the mid and hindgut transcriptomes showed only mild effects on metabolism. Flesh of fish fed the DCO diet accumulated almost double the amount of n-3 LC-PUFA than fish fed the FO or WCO diets, indicating that these oils from transgenic oilseeds offer the opportunity to increase the n-3 LC-PUFA in farmed fish to levels comparable to those found a decade ago. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/28403232/An_oil_containing_EPA_and_DHA_from_transgenic_Camelina_sativa_to_replace_marine_fish_oil_in_feeds_for_Atlantic_salmon__Salmo_salar_L__:_Effects_on_intestinal_transcriptome_histology_tissue_fatty_acid_profiles_and_plasma_biochemistry_ L2 - http://dx.plos.org/10.1371/journal.pone.0175415 DB - PRIME DP - Unbound Medicine ER -