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Auxin signaling through SCFTIR1/AFBs mediates feedback regulation of IAA biosynthesis.
Biosci Biotechnol Biochem. 2017 Jul; 81(7):1320-1326.BB

Abstract

We previously reported that exogenous application of auxin to Arabidopsis seedlings resulted in downregulation of indole-3-acetic acid (IAA) biosynthesis genes in a feedback manner. In this study, we investigated the involvement of the SCFTIR1/AFB-mediated signaling pathway in feedback regulation of the indole-3-pyruvic acid-mediated auxin biosynthesis pathway in Arabidopsis. Application of PEO-IAA, an inhibitor of the IAA signal transduction pathway, to wild-type seedlings resulted in increased endogenous IAA levels in roots. Endogenous IAA levels in the auxin-signaling mutants axr2-1, axr3-3, and tir1-1afb1-1afb2-1afb3-1 also increased. Furthermore, YUCCA (YUC) gene expression was repressed in response to auxin treatment, and expression of YUC7 and YUC8 increased in response to PEO-IAA treatment. YUC genes were also induced in auxin-signaling mutants but repressed in TIR1-overexpression lines. These observations suggest that the endogenous IAA levels are regulated by auxin biosynthesis in a feedback manner, and the Aux/IAA and SCFTIR1/AFB-mediated auxin-signaling pathway regulates the expression of YUC genes.

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Publisher Full Text
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Authors+Show Affiliations

Takato S
a Kihara Institute for Biological Research, Yokohama City University , Yokohama , Japan.
Kakei Y
a Kihara Institute for Biological Research, Yokohama City University , Yokohama , Japan.
Mitsui M
a Kihara Institute for Biological Research, Yokohama City University , Yokohama , Japan. b RIKEN CSRS , Yokohama , Japan.
Ishida Y
a Kihara Institute for Biological Research, Yokohama City University , Yokohama , Japan.
Suzuki M
a Kihara Institute for Biological Research, Yokohama City University , Yokohama , Japan.
Yamazaki C
a Kihara Institute for Biological Research, Yokohama City University , Yokohama , Japan.
Hayashi KI
c Department of Biochemistry , Okayama University of Science , Okayama , Japan.
Ishii T
d NARO/WARC , Zentsuji , Japan.
Nakamura A
a Kihara Institute for Biological Research, Yokohama City University , Yokohama , Japan.
Soeno K
d NARO/WARC , Zentsuji , Japan.
Shimada Y
a Kihara Institute for Biological Research, Yokohama City University , Yokohama , Japan. b RIKEN CSRS , Yokohama , Japan.

MeSH

ArabidopsisArabidopsis ProteinsCarrier ProteinsF-Box ProteinsFeedback, PhysiologicalGene Expression Regulation, PlantIndoleacetic AcidsIndolesMixed Function OxygenasesNuclear ProteinsOxygenasesPlant Growth RegulatorsPlant RootsReceptors, Cell SurfaceSeedlingsSignal TransductionTranscription Factors

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28406060

Citation

Takato, Shin, et al. "Auxin Signaling Through SCFTIR1/AFBs Mediates Feedback Regulation of IAA Biosynthesis." Bioscience, Biotechnology, and Biochemistry, vol. 81, no. 7, 2017, pp. 1320-1326.
Takato S, Kakei Y, Mitsui M, et al. Auxin signaling through SCFTIR1/AFBs mediates feedback regulation of IAA biosynthesis. Biosci Biotechnol Biochem. 2017;81(7):1320-1326.
Takato, S., Kakei, Y., Mitsui, M., Ishida, Y., Suzuki, M., Yamazaki, C., Hayashi, K. I., Ishii, T., Nakamura, A., Soeno, K., & Shimada, Y. (2017). Auxin signaling through SCFTIR1/AFBs mediates feedback regulation of IAA biosynthesis. Bioscience, Biotechnology, and Biochemistry, 81(7), 1320-1326. https://doi.org/10.1080/09168451.2017.1313694
Takato S, et al. Auxin Signaling Through SCFTIR1/AFBs Mediates Feedback Regulation of IAA Biosynthesis. Biosci Biotechnol Biochem. 2017;81(7):1320-1326. PubMed PMID: 28406060.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Auxin signaling through SCFTIR1/AFBs mediates feedback regulation of IAA biosynthesis. AU - Takato,Shin, AU - Kakei,Yusuke, AU - Mitsui,Marie, AU - Ishida,Yosuke, AU - Suzuki,Masashi, AU - Yamazaki,Chiaki, AU - Hayashi,Ken-Ichiro, AU - Ishii,Takahiro, AU - Nakamura,Ayako, AU - Soeno,Kazuo, AU - Shimada,Yukihisa, Y1 - 2017/04/13/ PY - 2017/4/14/pubmed PY - 2017/7/8/medline PY - 2017/4/14/entrez KW - Arabidopsis KW - auxin KW - feedback-regulation SP - 1320 EP - 1326 JF - Bioscience, biotechnology, and biochemistry JO - Biosci Biotechnol Biochem VL - 81 IS - 7 N2 - We previously reported that exogenous application of auxin to Arabidopsis seedlings resulted in downregulation of indole-3-acetic acid (IAA) biosynthesis genes in a feedback manner. In this study, we investigated the involvement of the SCFTIR1/AFB-mediated signaling pathway in feedback regulation of the indole-3-pyruvic acid-mediated auxin biosynthesis pathway in Arabidopsis. Application of PEO-IAA, an inhibitor of the IAA signal transduction pathway, to wild-type seedlings resulted in increased endogenous IAA levels in roots. Endogenous IAA levels in the auxin-signaling mutants axr2-1, axr3-3, and tir1-1afb1-1afb2-1afb3-1 also increased. Furthermore, YUCCA (YUC) gene expression was repressed in response to auxin treatment, and expression of YUC7 and YUC8 increased in response to PEO-IAA treatment. YUC genes were also induced in auxin-signaling mutants but repressed in TIR1-overexpression lines. These observations suggest that the endogenous IAA levels are regulated by auxin biosynthesis in a feedback manner, and the Aux/IAA and SCFTIR1/AFB-mediated auxin-signaling pathway regulates the expression of YUC genes. SN - 1347-6947 UR - https://www.unboundmedicine.com/medline/citation/28406060/Auxin_signaling_through_SCFTIR1/AFBs_mediates_feedback_regulation_of_IAA_biosynthesis_ L2 - https://academic.oup.com/bbb/article-lookup/doi/10.1080/09168451.2017.1313694 DB - PRIME DP - Unbound Medicine ER -