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Exposure to lethal levels of benzo[a]pyrene or cadmium trigger distinct protein expression patterns in earthworms (Eisenia fetida).
Sci Total Environ. 2017 Oct 01; 595:733-742.ST

Abstract

Different pollutants induce distinct toxic responses in earthworms (Eisenia fetida). Here, we used proteomics techniques to compare the responses of E. fetida to exposure to the 10% lethal concentration (14d-LC10) of benzo[a]pyrene (BaP) or cadmium (Cd) in natural red soil (China). BaP exposure markedly induced the expression of oxidation-reduction proteins, whereas Cd exposure mainly induced the expression of proteins involved in transcription- and translation-related processes. Furthermore, calmodulin-binding proteins were differentially expressed upon exposure to different pollutants. The calcium (Ca2+)-binding cytoskeletal element myosin was down-regulated upon BaP treatment, whereas the Ca2+-binding cytoskeletal element tropomyosin-1 was up-regulated upon Cd treatment. Some proteins exhibited opposite responses to the two pollutants. For instance, catalase (CAT) and heat shock protein 70 were up-regulated upon BaP treatment and down-regulated upon Cd treatment. A significant (p<0.05, one-way ANOVA with least-significant difference (LSD) test) increase in the level of reactive oxygen species (ROS) and CAT activity further showed that BaP mainly induces oxidative stress. Real-time PCR analysis showed that mRNA expression often did not correlate well with protein expression in earthworms subjected to Cd or BaP treatment. In addition, the expression of the gene encoding the protein metallothionein, which was not detected in the protein analysis, was induced upon Cd treatment, but slightly reduced upon BaP treatment. Therefore, BaP and Cd have distinct effects on the protein profile of E. Fetida with BaP markedly inducing ROS activity, and Cd mainly triggering genotoxicity.

CAPSULE SUMMARY

Distinct patterns of protein expression are induced in earthworms upon exposure to different pollutants; BaP markedly induces high levels of ROS, while Cd resultes in genotoxicity.

Authors+Show Affiliations

College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, People's Republic of China; Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing 210014, People's Republic of China.College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, People's Republic of China; College of Resources, Environment, and Planning, Dezhou University, Dezhou 253023, People's Republic of China.College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, People's Republic of China.Nanjing Scientific Institute of Vegetables and Flowers, Nanjing 210095, People's Republic of China.College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, People's Republic of China; Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing 210014, People's Republic of China. Electronic address: xuli602@njau.edu.cn.College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, People's Republic of China. Electronic address: huixinli@njau.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28407590

Citation

Zhang, Lihao, et al. "Exposure to Lethal Levels of Benzo[a]pyrene or Cadmium Trigger Distinct Protein Expression Patterns in Earthworms (Eisenia Fetida)." The Science of the Total Environment, vol. 595, 2017, pp. 733-742.
Zhang L, Duan X, He N, et al. Exposure to lethal levels of benzo[a]pyrene or cadmium trigger distinct protein expression patterns in earthworms (Eisenia fetida). Sci Total Environ. 2017;595:733-742.
Zhang, L., Duan, X., He, N., Chen, X., Shi, J., Li, W., Xu, L., & Li, H. (2017). Exposure to lethal levels of benzo[a]pyrene or cadmium trigger distinct protein expression patterns in earthworms (Eisenia fetida). The Science of the Total Environment, 595, 733-742. https://doi.org/10.1016/j.scitotenv.2017.04.003
Zhang L, et al. Exposure to Lethal Levels of Benzo[a]pyrene or Cadmium Trigger Distinct Protein Expression Patterns in Earthworms (Eisenia Fetida). Sci Total Environ. 2017 Oct 1;595:733-742. PubMed PMID: 28407590.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Exposure to lethal levels of benzo[a]pyrene or cadmium trigger distinct protein expression patterns in earthworms (Eisenia fetida). AU - Zhang,Lihao, AU - Duan,Xiaochen, AU - He,Nannan, AU - Chen,Xu, AU - Shi,Jinli, AU - Li,Weiming, AU - Xu,Li, AU - Li,Huixin, Y1 - 2017/04/11/ PY - 2016/12/22/received PY - 2017/03/30/revised PY - 2017/04/01/accepted PY - 2017/4/14/pubmed PY - 2018/7/31/medline PY - 2017/4/14/entrez KW - Benzo[a]pyrene KW - Cadmium KW - Eisenia fetida KW - Proteomics KW - Same lethal level KW - Toxic responses SP - 733 EP - 742 JF - The Science of the total environment JO - Sci Total Environ VL - 595 N2 - : Different pollutants induce distinct toxic responses in earthworms (Eisenia fetida). Here, we used proteomics techniques to compare the responses of E. fetida to exposure to the 10% lethal concentration (14d-LC10) of benzo[a]pyrene (BaP) or cadmium (Cd) in natural red soil (China). BaP exposure markedly induced the expression of oxidation-reduction proteins, whereas Cd exposure mainly induced the expression of proteins involved in transcription- and translation-related processes. Furthermore, calmodulin-binding proteins were differentially expressed upon exposure to different pollutants. The calcium (Ca2+)-binding cytoskeletal element myosin was down-regulated upon BaP treatment, whereas the Ca2+-binding cytoskeletal element tropomyosin-1 was up-regulated upon Cd treatment. Some proteins exhibited opposite responses to the two pollutants. For instance, catalase (CAT) and heat shock protein 70 were up-regulated upon BaP treatment and down-regulated upon Cd treatment. A significant (p<0.05, one-way ANOVA with least-significant difference (LSD) test) increase in the level of reactive oxygen species (ROS) and CAT activity further showed that BaP mainly induces oxidative stress. Real-time PCR analysis showed that mRNA expression often did not correlate well with protein expression in earthworms subjected to Cd or BaP treatment. In addition, the expression of the gene encoding the protein metallothionein, which was not detected in the protein analysis, was induced upon Cd treatment, but slightly reduced upon BaP treatment. Therefore, BaP and Cd have distinct effects on the protein profile of E. Fetida with BaP markedly inducing ROS activity, and Cd mainly triggering genotoxicity. CAPSULE SUMMARY: Distinct patterns of protein expression are induced in earthworms upon exposure to different pollutants; BaP markedly induces high levels of ROS, while Cd resultes in genotoxicity. SN - 1879-1026 UR - https://www.unboundmedicine.com/medline/citation/28407590/Exposure_to_lethal_levels_of_benzo[a]pyrene_or_cadmium_trigger_distinct_protein_expression_patterns_in_earthworms__Eisenia_fetida__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0048-9697(17)30830-6 DB - PRIME DP - Unbound Medicine ER -