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Dietary Docosahexaenoic Acid and trans-10, cis-12-Conjugated Linoleic Acid Differentially Alter Oxylipin Profiles in Mouse Periuterine Adipose Tissue.
Lipids. 2017 05; 52(5):399-413.L

Abstract

Diets containing high n-3 polyunsaturated fatty acids (PUFA) decrease inflammation and the incidence of chronic diseases including cardiovascular disease and nonalcoholic fatty liver disease while trans-fatty acids (TFA) intake increases the incidence of these conditions. Some health benefits of n-3 PUFA are mediated through the impact of their oxygenated metabolites, i.e. oxylipins. The TFA, trans-10, cis-12-conjugated linoleic acid (CLA; 18:2n-6) is associated with adipose tissue (AT) inflammation, oxidative stress, and wasting. We examined the impact of a 4-week feeding of 0, 0.5, and 1.5% docosahexaenoic acid (DHA; 22:6n-3) in the presence and absence of 0.5% CLA on AT oxylipin profiles in female C57BL/6N mice. Esterified oxylipins in AT derived from linoleic acid (LNA), alpha-linolenic acid (ALA), arachidonic acid (ARA), eicosapentaenoic acid (EPA), DHA, and putative from CLA were quantified. CLA containing diets reduced AT mass by ~62%. Compared with the control diet, the DHA diet elevated concentrations of EPA-and DHA-derived alcohols and epoxides and LNA-derived alcohols, reduced ARA-derived alcohols, ketones, epoxides, and 6-keto-prostaglandin (PG) F1α (P < 0.05), and had mixed effects on ALA-derived alcohols. Dietary CLA lowered EPA-, DHA-, and ALA-derived epoxides, ARA-derived ketones and epoxides, and ALA-derived alcohols. While dietary CLA induced variable effects in EPA-, DHA-, and LNA-derived alcohols and LNA-derived ketones, it elevated ARA-derived alcohols and PGF1α, PGF2α, and F2-isoprostanes. DHA counteracted CLA-induced effects in 67, 57, 43, and 29% of total DHA-, ARA-, EPA-, and ALA-derived oxylipins, respectively. Thus, CLA elevated proinflammatory oxylipins while DHA increased anti-inflammatory oxylipins and diminished concentration of CLA-induced pro-inflammatory oxylipins in AT.

Authors+Show Affiliations

Western Human Nutrition Research Center, Agricultural Research Service, United States Department of Agriculture, 430 West Health Sciences Drive, Davis, CA, 95616, USA. Department of Nutrition, University of California, Davis, CA, 95616, USA.Western Human Nutrition Research Center, Agricultural Research Service, United States Department of Agriculture, 430 West Health Sciences Drive, Davis, CA, 95616, USA.Western Human Nutrition Research Center, Agricultural Research Service, United States Department of Agriculture, 430 West Health Sciences Drive, Davis, CA, 95616, USA.Western Human Nutrition Research Center, Agricultural Research Service, United States Department of Agriculture, 430 West Health Sciences Drive, Davis, CA, 95616, USA. Department of Nutrition, University of California, Davis, CA, 95616, USA.Western Regional Research Center, Agricultural Research Service, United States Department of Agriculture, Albany, CA, 94710, USA.Western Human Nutrition Research Center, Agricultural Research Service, United States Department of Agriculture, 430 West Health Sciences Drive, Davis, CA, 95616, USA. Department of Nutrition, University of California, Davis, CA, 95616, USA.Western Human Nutrition Research Center, Agricultural Research Service, United States Department of Agriculture, 430 West Health Sciences Drive, Davis, CA, 95616, USA. darshan.kelley@ars.usda.gov. Department of Nutrition, University of California, Davis, CA, 95616, USA. darshan.kelley@ars.usda.gov.

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

28409336

Citation

Adkins, Yuriko, et al. "Dietary Docosahexaenoic Acid and Trans-10, cis-12-Conjugated Linoleic Acid Differentially Alter Oxylipin Profiles in Mouse Periuterine Adipose Tissue." Lipids, vol. 52, no. 5, 2017, pp. 399-413.
Adkins Y, Belda BJ, Pedersen TL, et al. Dietary Docosahexaenoic Acid and trans-10, cis-12-Conjugated Linoleic Acid Differentially Alter Oxylipin Profiles in Mouse Periuterine Adipose Tissue. Lipids. 2017;52(5):399-413.
Adkins, Y., Belda, B. J., Pedersen, T. L., Fedor, D. M., Mackey, B. E., Newman, J. W., & Kelley, D. S. (2017). Dietary Docosahexaenoic Acid and trans-10, cis-12-Conjugated Linoleic Acid Differentially Alter Oxylipin Profiles in Mouse Periuterine Adipose Tissue. Lipids, 52(5), 399-413. https://doi.org/10.1007/s11745-017-4252-3
Adkins Y, et al. Dietary Docosahexaenoic Acid and Trans-10, cis-12-Conjugated Linoleic Acid Differentially Alter Oxylipin Profiles in Mouse Periuterine Adipose Tissue. Lipids. 2017;52(5):399-413. PubMed PMID: 28409336.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dietary Docosahexaenoic Acid and trans-10, cis-12-Conjugated Linoleic Acid Differentially Alter Oxylipin Profiles in Mouse Periuterine Adipose Tissue. AU - Adkins,Yuriko, AU - Belda,Benjamin J, AU - Pedersen,Theresa L, AU - Fedor,Dawn M, AU - Mackey,Bruce E, AU - Newman,John W, AU - Kelley,Darshan S, Y1 - 2017/04/13/ PY - 2016/08/18/received PY - 2017/04/04/accepted PY - 2017/4/15/pubmed PY - 2018/2/14/medline PY - 2017/4/15/entrez KW - CLA KW - DHA KW - Inflammation KW - Oxylipins KW - n-3 PUFA SP - 399 EP - 413 JF - Lipids JO - Lipids VL - 52 IS - 5 N2 - Diets containing high n-3 polyunsaturated fatty acids (PUFA) decrease inflammation and the incidence of chronic diseases including cardiovascular disease and nonalcoholic fatty liver disease while trans-fatty acids (TFA) intake increases the incidence of these conditions. Some health benefits of n-3 PUFA are mediated through the impact of their oxygenated metabolites, i.e. oxylipins. The TFA, trans-10, cis-12-conjugated linoleic acid (CLA; 18:2n-6) is associated with adipose tissue (AT) inflammation, oxidative stress, and wasting. We examined the impact of a 4-week feeding of 0, 0.5, and 1.5% docosahexaenoic acid (DHA; 22:6n-3) in the presence and absence of 0.5% CLA on AT oxylipin profiles in female C57BL/6N mice. Esterified oxylipins in AT derived from linoleic acid (LNA), alpha-linolenic acid (ALA), arachidonic acid (ARA), eicosapentaenoic acid (EPA), DHA, and putative from CLA were quantified. CLA containing diets reduced AT mass by ~62%. Compared with the control diet, the DHA diet elevated concentrations of EPA-and DHA-derived alcohols and epoxides and LNA-derived alcohols, reduced ARA-derived alcohols, ketones, epoxides, and 6-keto-prostaglandin (PG) F1α (P < 0.05), and had mixed effects on ALA-derived alcohols. Dietary CLA lowered EPA-, DHA-, and ALA-derived epoxides, ARA-derived ketones and epoxides, and ALA-derived alcohols. While dietary CLA induced variable effects in EPA-, DHA-, and LNA-derived alcohols and LNA-derived ketones, it elevated ARA-derived alcohols and PGF1α, PGF2α, and F2-isoprostanes. DHA counteracted CLA-induced effects in 67, 57, 43, and 29% of total DHA-, ARA-, EPA-, and ALA-derived oxylipins, respectively. Thus, CLA elevated proinflammatory oxylipins while DHA increased anti-inflammatory oxylipins and diminished concentration of CLA-induced pro-inflammatory oxylipins in AT. SN - 1558-9307 UR - https://www.unboundmedicine.com/medline/citation/28409336/Dietary_Docosahexaenoic_Acid_and_trans_10_cis_12_Conjugated_Linoleic_Acid_Differentially_Alter_Oxylipin_Profiles_in_Mouse_Periuterine_Adipose_Tissue_ L2 - https://link.springer.com/article/10.1007/s11745-017-4252-3 DB - PRIME DP - Unbound Medicine ER -