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Extensive diversity of rickettsiales bacteria in ticks from Wuhan, China.
Ticks Tick Borne Dis 2017; 8(4):574-580TT

Abstract

Rickettsiales bacteria are important agents of (re)emerging infectious diseases, with ticks playing a key role in their evolution and transmission. We collected 1079 hard ticks belonging to five species (Ixodes sinensis, Rhipicephalus microplus, Haemaphysalis flava, Haemaphysalis hystricis and Haemaphysalis longicornis) from cattle and goats in Wuhan city, Hubei province, China. The dominant tick species was H. longicornis (578, 53.57%), followed by R. microplus (354, 32.81%), H. hystricis (62, 5.75%), H. flava (57, 5.28%), and I. sinensis (28, 2.59%). Rickettsiales bacteria were identified in these ticks by amplifying the Rickettsiales 16S rRNA (rrs), citrate synthase (gltA), and heat shock protein (groEL) genes. The rrs gene of Rickettsiales was positive in 32 (2.97%) ticks, including 2 cases of co-infection, with 4 (0.69%) in H. longicornis, 15 (4.24%) in R. microplus, 7 (12.28%) in H. flava, 1 (1.61%) in H. hystricis, and 5 (17.86%) in I. sinensis ticks. Phylogenetic analysis revealed the presence of six recognized and seven Candidatus species of Rickettsiaceae, Anaplasmataceae and Candidatus Midichloriaceae. Notably, one lineage within both Ehrlichia and Candidatus Midichloriaceae was distinct from any known Rickettsiales, suggesting the presence of potentially novel species of Rickettsiales bacteria. In sum, these data reveal an extensive diversity of Rickettsiales in ticks from Wuhan, highlighting the need to understand Rickettsiales infection in local animals and humans.

Authors+Show Affiliations

State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Department of Zoonoses, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.Wuhan Center for Disease Control and Prevention, Wuhan, Hubei Province, China.Wuhan Center for Disease Control and Prevention, Wuhan, Hubei Province, China.State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Department of Zoonoses, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Department of Zoonoses, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China; Marie Bashir Institute for Infectious Diseases and Biosecurity, Charles Perkins Centre, School of Life and Environmental Sciences and Sydney Medical School, The University of Sydney, Sydney, NSW 2006, Australia.State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Department of Zoonoses, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China. Electronic address: zhangyongzhen@icdc.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

28411028

Citation

Lu, Miao, et al. "Extensive Diversity of Rickettsiales Bacteria in Ticks From Wuhan, China." Ticks and Tick-borne Diseases, vol. 8, no. 4, 2017, pp. 574-580.
Lu M, Tian JH, Yu B, et al. Extensive diversity of rickettsiales bacteria in ticks from Wuhan, China. Ticks Tick Borne Dis. 2017;8(4):574-580.
Lu, M., Tian, J. H., Yu, B., Guo, W. P., Holmes, E. C., & Zhang, Y. Z. (2017). Extensive diversity of rickettsiales bacteria in ticks from Wuhan, China. Ticks and Tick-borne Diseases, 8(4), pp. 574-580. doi:10.1016/j.ttbdis.2017.03.006.
Lu M, et al. Extensive Diversity of Rickettsiales Bacteria in Ticks From Wuhan, China. Ticks Tick Borne Dis. 2017;8(4):574-580. PubMed PMID: 28411028.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Extensive diversity of rickettsiales bacteria in ticks from Wuhan, China. AU - Lu,Miao, AU - Tian,Jun-Hua, AU - Yu,Bin, AU - Guo,Wen-Ping, AU - Holmes,Edward C, AU - Zhang,Yong-Zhen, Y1 - 2017/03/24/ PY - 2016/08/31/received PY - 2017/03/01/revised PY - 2017/03/22/accepted PY - 2017/4/16/pubmed PY - 2018/3/17/medline PY - 2017/4/16/entrez KW - China KW - Emerging disease KW - Evolution KW - Phylogeny KW - Rickettsiales bacteria KW - Ticks SP - 574 EP - 580 JF - Ticks and tick-borne diseases JO - Ticks Tick Borne Dis VL - 8 IS - 4 N2 - Rickettsiales bacteria are important agents of (re)emerging infectious diseases, with ticks playing a key role in their evolution and transmission. We collected 1079 hard ticks belonging to five species (Ixodes sinensis, Rhipicephalus microplus, Haemaphysalis flava, Haemaphysalis hystricis and Haemaphysalis longicornis) from cattle and goats in Wuhan city, Hubei province, China. The dominant tick species was H. longicornis (578, 53.57%), followed by R. microplus (354, 32.81%), H. hystricis (62, 5.75%), H. flava (57, 5.28%), and I. sinensis (28, 2.59%). Rickettsiales bacteria were identified in these ticks by amplifying the Rickettsiales 16S rRNA (rrs), citrate synthase (gltA), and heat shock protein (groEL) genes. The rrs gene of Rickettsiales was positive in 32 (2.97%) ticks, including 2 cases of co-infection, with 4 (0.69%) in H. longicornis, 15 (4.24%) in R. microplus, 7 (12.28%) in H. flava, 1 (1.61%) in H. hystricis, and 5 (17.86%) in I. sinensis ticks. Phylogenetic analysis revealed the presence of six recognized and seven Candidatus species of Rickettsiaceae, Anaplasmataceae and Candidatus Midichloriaceae. Notably, one lineage within both Ehrlichia and Candidatus Midichloriaceae was distinct from any known Rickettsiales, suggesting the presence of potentially novel species of Rickettsiales bacteria. In sum, these data reveal an extensive diversity of Rickettsiales in ticks from Wuhan, highlighting the need to understand Rickettsiales infection in local animals and humans. SN - 1877-9603 UR - https://www.unboundmedicine.com/medline/citation/28411028/Extensive_diversity_of_rickettsiales_bacteria_in_ticks_from_Wuhan_China_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1877-959X(17)30141-3 DB - PRIME DP - Unbound Medicine ER -