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Expression profiling-based clustering of healthy subjects recapitulates classifications defined by clinical observation in Chinese medicine.
J Genet Genomics. 2017 04 20; 44(4):191-197.JG

Abstract

Differences between healthy subjects and associated disease risks are of substantial interest in clinical medicine. Based on clinical presentations, Traditional Chinese Medicine (TCM) classifies healthy people into nine constitutions: Balanced, Qi, Yang or Yin deficiency, Phlegm-dampness, Damp-heat, Blood stasis, Qi stagnation, and Inherited special constitutions. In particular, Yang and Yin deficiency constitutions exhibit cold and heat aversion, respectively. However, the intrinsic molecular characteristics of unbalanced phenotypes remain unclear. To determine whether gene expression-based clustering can recapitulate TCM-based classification, peripheral blood mononuclear cells (PBMCs) were collected from Chinese Han individuals with Yang/Yin deficiency (n = 12 each) and Balanced (n = 8) constitutions, and global gene expression profiles were determined using the Affymetrix HG-U133A Plus 2.0 array. Notably, we found that gene expression-based classifications reflected distinct TCM-based subtypes. Consistent with the clinical observation that subjects with Yang deficiency tend toward obesity, series-clustering analysis detected several key lipid metabolic genes (diacylglycerol acyltransferase (DGAT2), acyl-CoA synthetase (ACSL1), and ATP-binding cassette subfamily A member 1 (ABCA1)) to be down- and up-regulated in Yin and Yang deficiency constitutions, respectively. Our findings suggest that Yin/Yang deficiency and Balanced constitutions are unique entities in their mRNA expression profiles. Moreover, the distinct physical and clinical characteristics of each unbalanced constitution can be explained, in part, by specific gene expression signatures.

Authors+Show Affiliations

Health Preservation Institute, School of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China.Key Laboratory of Genomics and Precision Medicine, Collaborative Innovation Center of Genetics and Development, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China.Center for Studies in Constitution Research of Traditional Chinese Medicine, School of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China.Center for Studies in Constitution Research of Traditional Chinese Medicine, School of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China.Center for Studies in Constitution Research of Traditional Chinese Medicine, School of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China.Center for Studies in Constitution Research of Traditional Chinese Medicine, School of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China.Center for Studies in Constitution Research of Traditional Chinese Medicine, School of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China.Center for Studies in Constitution Research of Traditional Chinese Medicine, School of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China.Center for Studies in Constitution Research of Traditional Chinese Medicine, School of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China.Key Laboratory of Genomics and Precision Medicine, Collaborative Innovation Center of Genetics and Development, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address: wangqf@big.ac.cn.Center for Studies in Constitution Research of Traditional Chinese Medicine, School of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China. Electronic address: wangqi710@126.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

28412226

Citation

Yu, Ruoxi, et al. "Expression Profiling-based Clustering of Healthy Subjects Recapitulates Classifications Defined By Clinical Observation in Chinese Medicine." Journal of Genetics and Genomics = Yi Chuan Xue Bao, vol. 44, no. 4, 2017, pp. 191-197.
Yu R, Liu D, Yang Y, et al. Expression profiling-based clustering of healthy subjects recapitulates classifications defined by clinical observation in Chinese medicine. J Genet Genomics. 2017;44(4):191-197.
Yu, R., Liu, D., Yang, Y., Han, Y., Li, L., Zheng, L., Wang, J., Zhang, Y., Li, Y., Wang, Q. F., & Wang, Q. (2017). Expression profiling-based clustering of healthy subjects recapitulates classifications defined by clinical observation in Chinese medicine. Journal of Genetics and Genomics = Yi Chuan Xue Bao, 44(4), 191-197. https://doi.org/10.1016/j.jgg.2017.01.001
Yu R, et al. Expression Profiling-based Clustering of Healthy Subjects Recapitulates Classifications Defined By Clinical Observation in Chinese Medicine. J Genet Genomics. 2017 04 20;44(4):191-197. PubMed PMID: 28412226.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression profiling-based clustering of healthy subjects recapitulates classifications defined by clinical observation in Chinese medicine. AU - Yu,Ruoxi, AU - Liu,Dan, AU - Yang,Yin, AU - Han,Yuanyuan, AU - Li,Lingru, AU - Zheng,Luyu, AU - Wang,Ji, AU - Zhang,Yan, AU - Li,Yingshuai, AU - Wang,Qian-Fei, AU - Wang,Qi, Y1 - 2017/01/17/ PY - 2016/08/12/received PY - 2017/01/10/revised PY - 2017/01/13/accepted PY - 2017/4/17/pubmed PY - 2017/12/26/medline PY - 2017/4/17/entrez KW - Classification KW - Constitution KW - Gene expression KW - Traditional Chinese Medicine SP - 191 EP - 197 JF - Journal of genetics and genomics = Yi chuan xue bao JO - J Genet Genomics VL - 44 IS - 4 N2 - Differences between healthy subjects and associated disease risks are of substantial interest in clinical medicine. Based on clinical presentations, Traditional Chinese Medicine (TCM) classifies healthy people into nine constitutions: Balanced, Qi, Yang or Yin deficiency, Phlegm-dampness, Damp-heat, Blood stasis, Qi stagnation, and Inherited special constitutions. In particular, Yang and Yin deficiency constitutions exhibit cold and heat aversion, respectively. However, the intrinsic molecular characteristics of unbalanced phenotypes remain unclear. To determine whether gene expression-based clustering can recapitulate TCM-based classification, peripheral blood mononuclear cells (PBMCs) were collected from Chinese Han individuals with Yang/Yin deficiency (n = 12 each) and Balanced (n = 8) constitutions, and global gene expression profiles were determined using the Affymetrix HG-U133A Plus 2.0 array. Notably, we found that gene expression-based classifications reflected distinct TCM-based subtypes. Consistent with the clinical observation that subjects with Yang deficiency tend toward obesity, series-clustering analysis detected several key lipid metabolic genes (diacylglycerol acyltransferase (DGAT2), acyl-CoA synthetase (ACSL1), and ATP-binding cassette subfamily A member 1 (ABCA1)) to be down- and up-regulated in Yin and Yang deficiency constitutions, respectively. Our findings suggest that Yin/Yang deficiency and Balanced constitutions are unique entities in their mRNA expression profiles. Moreover, the distinct physical and clinical characteristics of each unbalanced constitution can be explained, in part, by specific gene expression signatures. SN - 1673-8527 UR - https://www.unboundmedicine.com/medline/citation/28412226/Expression_profiling_based_clustering_of_healthy_subjects_recapitulates_classifications_defined_by_clinical_observation_in_Chinese_medicine_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1673-8527(17)30014-0 DB - PRIME DP - Unbound Medicine ER -