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Levan promotes antiproliferative and pro-apoptotic effects in MCF-7 breast cancer cells mediated by oxidative stress.
Int J Biol Macromol. 2017 Sep; 102:565-570.IJ

Abstract

Exopolysaccharides are high-valued bio-products produced by various microbial species and have been described to possess biological response modifying activities. These bio-products have been effective as therapeutic agents in various human disease conditions. The objective of this study was to examine the effects of levan (a (2→6)-β-d-fructan) produced on sucrose by the halophilic bacterium, Halomonas smyrnensis AAD6T, in human breast cancer MCF-7 cells. MCF-7 cells were exposed to levan for 24 and 48h. The antiproliferative activity was analyzed by the MTT assay. Oxidative stress was measured by the CM-H2DCFDA assay, and cell apoptosis was analyzed by the caspase-3/7 assay. Cell cycle was analyzed by flow cytometry and gene expression was determined by RT-PCR. Levan showed a time- and concentration-dependent antiproliferative activity, and this effect was associated with an increase in cell apoptosis and oxidative stress. In addition, levan increased the gene expression of p53 and p27. Here we demonstrated that levan exhibited an antiproliferative effect that was mediated by an increase in apoptosis and oxidative stress.

Authors+Show Affiliations

Departamento de Farmacologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, SP, Brazil; Biorefining Research Institute, Lakehead University, Thunder Bay, ON, P7 B 5E1, Canada; Medical Sciences Division, Northern Ontario School of Medicine (NOSM), Lakehead University, Thunder Bay, ON, P7 B 5E1, Canada; Unidade de Pesquisa Experimental, Instituto de Ciências da Saúde, Universidade Federal de Mato Grosso, Sinop, MT, Brazil.Departamento de Farmacologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, SP, Brazil.Departamento de Química, Universidade Tecnológica Federal do Paraná, Pato Branco, SP, Brazil.IBSB, Department of Bioengineering, Marmara University, Goztepe, Istanbul, 34722, Turkey.Biorefining Research Institute, Lakehead University, Thunder Bay, ON, P7 B 5E1, Canada; Universidade Estadual de Londrina, Departamento de Química, CCE, Londrina, PR, Brazil.IBSB, Department of Bioengineering, Marmara University, Goztepe, Istanbul, 34722, Turkey.Biorefining Research Institute, Lakehead University, Thunder Bay, ON, P7 B 5E1, Canada; Universidade Tecnológica Federal do Paraná, Programa de Pós-Graduação em Engenharia Ambiental, Campus Londrina, Londrina, PR, Brazil.Medical Sciences Division, Northern Ontario School of Medicine (NOSM), Lakehead University, Thunder Bay, ON, P7 B 5E1, Canada. Electronic address: nkhaper@nosm.ca.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28412340

Citation

Queiroz, Eveline A I F., et al. "Levan Promotes Antiproliferative and Pro-apoptotic Effects in MCF-7 Breast Cancer Cells Mediated By Oxidative Stress." International Journal of Biological Macromolecules, vol. 102, 2017, pp. 565-570.
Queiroz EAIF, Fortes ZB, da Cunha MAA, et al. Levan promotes antiproliferative and pro-apoptotic effects in MCF-7 breast cancer cells mediated by oxidative stress. Int J Biol Macromol. 2017;102:565-570.
Queiroz, E. A. I. F., Fortes, Z. B., da Cunha, M. A. A., Sarilmiser, H. K., Barbosa Dekker, A. M., Öner, E. T., Dekker, R. F. H., & Khaper, N. (2017). Levan promotes antiproliferative and pro-apoptotic effects in MCF-7 breast cancer cells mediated by oxidative stress. International Journal of Biological Macromolecules, 102, 565-570. https://doi.org/10.1016/j.ijbiomac.2017.04.035
Queiroz EAIF, et al. Levan Promotes Antiproliferative and Pro-apoptotic Effects in MCF-7 Breast Cancer Cells Mediated By Oxidative Stress. Int J Biol Macromol. 2017;102:565-570. PubMed PMID: 28412340.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Levan promotes antiproliferative and pro-apoptotic effects in MCF-7 breast cancer cells mediated by oxidative stress. AU - Queiroz,Eveline A I F, AU - Fortes,Zuleica B, AU - da Cunha,Mário A A, AU - Sarilmiser,Hande Kazak, AU - Barbosa Dekker,Aneli M, AU - Öner,Ebru Toksoy, AU - Dekker,Robert F H, AU - Khaper,Neelam, Y1 - 2017/04/12/ PY - 2016/10/18/received PY - 2017/03/15/revised PY - 2017/04/10/accepted PY - 2017/4/17/pubmed PY - 2018/3/13/medline PY - 2017/4/17/entrez KW - (2→6)-β-d-Fructan KW - Apoptosis KW - Oxidative stress KW - p27 KW - p53 SP - 565 EP - 570 JF - International journal of biological macromolecules JO - Int J Biol Macromol VL - 102 N2 - Exopolysaccharides are high-valued bio-products produced by various microbial species and have been described to possess biological response modifying activities. These bio-products have been effective as therapeutic agents in various human disease conditions. The objective of this study was to examine the effects of levan (a (2→6)-β-d-fructan) produced on sucrose by the halophilic bacterium, Halomonas smyrnensis AAD6T, in human breast cancer MCF-7 cells. MCF-7 cells were exposed to levan for 24 and 48h. The antiproliferative activity was analyzed by the MTT assay. Oxidative stress was measured by the CM-H2DCFDA assay, and cell apoptosis was analyzed by the caspase-3/7 assay. Cell cycle was analyzed by flow cytometry and gene expression was determined by RT-PCR. Levan showed a time- and concentration-dependent antiproliferative activity, and this effect was associated with an increase in cell apoptosis and oxidative stress. In addition, levan increased the gene expression of p53 and p27. Here we demonstrated that levan exhibited an antiproliferative effect that was mediated by an increase in apoptosis and oxidative stress. SN - 1879-0003 UR - https://www.unboundmedicine.com/medline/citation/28412340/Levan_promotes_antiproliferative_and_pro_apoptotic_effects_in_MCF_7_breast_cancer_cells_mediated_by_oxidative_stress_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0141-8130(16)32065-7 DB - PRIME DP - Unbound Medicine ER -