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CdS:Mn quantum dot-functionalized g-C3N4 nanohybrids as signal-generation tags for photoelectrochemical immunoassay of prostate specific antigen coupling DNAzyme concatamer with enzymatic biocatalytic precipitation.
Biosens Bioelectron. 2017 Sep 15; 95:34-40.BB

Abstract

A new photoelectrochemical (PEC) immunosensor based on Mn-doped CdS quantum dots (CdS:Mn QDs) on g-C3N4 nanosheets was developed for the sensitive detection of prostate specific antibody (PSA) in biological fluids. The signal derived from the as-synthesized Cd:Mn QDs-functionalized g-C3N4 nanohybrids via a hydrothermal method and was amplified through DNAzyme concatamers on gold nanoparticles accompanying enzymatic biocatalytic precipitation. Experimental results by UV-vis absorption spectra and photoluminescence revealed that CdS:Mn QDs/g-C3N4 nanohybrids exhibited higher photocurrent than those of CdS:Mn QDs and g-C3N4 alone. Upon addition of target PSA, a sandwich-type immunoreaction was carried out between capture antibodies and the labeled detection antibodies. Accompanying introduction of gold nanoparticles, the labeled initiator strands on the AuNPs triggered hybridization chain reaction and the formation of DNAzyme concatamers in the presence of hemin. The formed DNAzyme catalyzed 4-chloro-1-naphthol (4-CN) to produce an insoluble/insulating precipitate on the Mn:CdS QDs/g-C3N4, and blocked the light harvesting of Mn:CdS QDs/g-C3N4, thus resulting in the decreasing photocurrent. Under optimal conditions, the immunosensor exhibited good photocurrent responses for determination of target PSA, and allowed detection of PSA at a concentration as low as 3.8pgmL[-1]. The specificity, reproducibility and precision of this system were acceptable. Significantly, this methodology was further evaluated for analyzing human serum samples, giving well-matched results with referenced PSA enzyme-linked immunosorbent assay (ELISA) method.

Authors+Show Affiliations

Key Laboratory of Analysis and Detection for Food Safety (MOE & Fujian Province), Collaborative Innovation Center of Detection Technology for Haixi Food Safety and Products (Fujian Province), State Key Laboratory of Photocatalysis on Energy and Environment, Department of Chemistry, Fuzhou University, Fuzhou 350116, PR China.Key Laboratory of Analysis and Detection for Food Safety (MOE & Fujian Province), Collaborative Innovation Center of Detection Technology for Haixi Food Safety and Products (Fujian Province), State Key Laboratory of Photocatalysis on Energy and Environment, Department of Chemistry, Fuzhou University, Fuzhou 350116, PR China.Key Laboratory of Analysis and Detection for Food Safety (MOE & Fujian Province), Collaborative Innovation Center of Detection Technology for Haixi Food Safety and Products (Fujian Province), State Key Laboratory of Photocatalysis on Energy and Environment, Department of Chemistry, Fuzhou University, Fuzhou 350116, PR China.Key Laboratory of Analysis and Detection for Food Safety (MOE & Fujian Province), Collaborative Innovation Center of Detection Technology for Haixi Food Safety and Products (Fujian Province), State Key Laboratory of Photocatalysis on Energy and Environment, Department of Chemistry, Fuzhou University, Fuzhou 350116, PR China. Electronic address: dianping.tang@fzu.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28412658

Citation

Zhang, Kangyao, et al. "CdS:Mn Quantum Dot-functionalized g-C3N4 Nanohybrids as Signal-generation Tags for Photoelectrochemical Immunoassay of Prostate Specific Antigen Coupling DNAzyme Concatamer With Enzymatic Biocatalytic Precipitation." Biosensors & Bioelectronics, vol. 95, 2017, pp. 34-40.
Zhang K, Lv S, Lin Z, et al. CdS:Mn quantum dot-functionalized g-C3N4 nanohybrids as signal-generation tags for photoelectrochemical immunoassay of prostate specific antigen coupling DNAzyme concatamer with enzymatic biocatalytic precipitation. Biosens Bioelectron. 2017;95:34-40.
Zhang, K., Lv, S., Lin, Z., & Tang, D. (2017). CdS:Mn quantum dot-functionalized g-C3N4 nanohybrids as signal-generation tags for photoelectrochemical immunoassay of prostate specific antigen coupling DNAzyme concatamer with enzymatic biocatalytic precipitation. Biosensors & Bioelectronics, 95, 34-40. https://doi.org/10.1016/j.bios.2017.04.005
Zhang K, et al. CdS:Mn Quantum Dot-functionalized g-C3N4 Nanohybrids as Signal-generation Tags for Photoelectrochemical Immunoassay of Prostate Specific Antigen Coupling DNAzyme Concatamer With Enzymatic Biocatalytic Precipitation. Biosens Bioelectron. 2017 Sep 15;95:34-40. PubMed PMID: 28412658.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - CdS:Mn quantum dot-functionalized g-C3N4 nanohybrids as signal-generation tags for photoelectrochemical immunoassay of prostate specific antigen coupling DNAzyme concatamer with enzymatic biocatalytic precipitation. AU - Zhang,Kangyao, AU - Lv,Shuzhen, AU - Lin,Zhenzhen, AU - Tang,Dianping, Y1 - 2017/04/12/ PY - 2017/01/16/received PY - 2017/03/10/revised PY - 2017/04/10/accepted PY - 2017/4/17/pubmed PY - 2018/2/15/medline PY - 2017/4/17/entrez KW - DNAzyme concatamers KW - Enzyme biocatalytic precipitation KW - G-C(3)N(4) nanosheets KW - Hybridization chain reaction KW - Mn-doped CdS quantum dots KW - Photoelectrochemical immunosensor SP - 34 EP - 40 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 95 N2 - A new photoelectrochemical (PEC) immunosensor based on Mn-doped CdS quantum dots (CdS:Mn QDs) on g-C3N4 nanosheets was developed for the sensitive detection of prostate specific antibody (PSA) in biological fluids. The signal derived from the as-synthesized Cd:Mn QDs-functionalized g-C3N4 nanohybrids via a hydrothermal method and was amplified through DNAzyme concatamers on gold nanoparticles accompanying enzymatic biocatalytic precipitation. Experimental results by UV-vis absorption spectra and photoluminescence revealed that CdS:Mn QDs/g-C3N4 nanohybrids exhibited higher photocurrent than those of CdS:Mn QDs and g-C3N4 alone. Upon addition of target PSA, a sandwich-type immunoreaction was carried out between capture antibodies and the labeled detection antibodies. Accompanying introduction of gold nanoparticles, the labeled initiator strands on the AuNPs triggered hybridization chain reaction and the formation of DNAzyme concatamers in the presence of hemin. The formed DNAzyme catalyzed 4-chloro-1-naphthol (4-CN) to produce an insoluble/insulating precipitate on the Mn:CdS QDs/g-C3N4, and blocked the light harvesting of Mn:CdS QDs/g-C3N4, thus resulting in the decreasing photocurrent. Under optimal conditions, the immunosensor exhibited good photocurrent responses for determination of target PSA, and allowed detection of PSA at a concentration as low as 3.8pgmL[-1]. The specificity, reproducibility and precision of this system were acceptable. Significantly, this methodology was further evaluated for analyzing human serum samples, giving well-matched results with referenced PSA enzyme-linked immunosorbent assay (ELISA) method. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/28412658/CdS:Mn_quantum_dot_functionalized_g_C3N4_nanohybrids_as_signal_generation_tags_for_photoelectrochemical_immunoassay_of_prostate_specific_antigen_coupling_DNAzyme_concatamer_with_enzymatic_biocatalytic_precipitation_ DB - PRIME DP - Unbound Medicine ER -