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Neuroprotective effect of Demethoxycurcumin, a natural derivative of Curcumin on rotenone induced neurotoxicity in SH-SY 5Y Neuroblastoma cells.
BMC Complement Altern Med. 2017 Apr 18; 17(1):217.BC

Abstract

BACKGROUND

Mitochondrial dysfunction and oxidative stress are the main toxic events leading to dopaminergic neuronal death in Parkinson's disease (PD) and identified as vital objective for therapeutic intercession. This study investigated the neuro-protective effects of the demethoxycurcumin (DMC), a derivative of curcumin against rotenone induced neurotoxicity.

METHODS

SH-SY5Y neuroblastoma cells are divided into four experimental groups: untreated cells, cells incubated with rotenone (100 nM), cells treated with DMC (50 nM) + rotenone (100 nM) and DMC alone treated. 24 h after treatment with rotenone and 28 h after treatment with DMC, cell viability was assessed using the MTT assay, and levels of ROS and MMP, plus expression of apoptotic protein were analysed.

RESULTS

Rotenone induced cell death in SH-SY5Y cells was significantly reduced by DMC pretreatment in a dose-dependent manner, indicating the potent neuroprotective effects of DMC. Rotenone treatment significantly increases the levels of ROS, loss of MMP, release of Cyt-c and expression of pro-apoptotic markers and decreases the expression of anti-apoptotic markers.

CONCLUSIONS

Even though the results of the present study indicated that the DMC may serve as a potent therapeutic agent particularly for the treatment of neurodegenerative diseases like PD, further pre-clinical and clinical studies are required.

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Authors+Show Affiliations

Ramkumar M
Research Scholar, Bharath University, Selaiyur, Chennai, Tamil Nadu, 600073, India.
Rajasankar S
Department of Anatomy, Velammal Medical College and Hospital, Madurai, Tamil Nadu, 625009, India. anatomysrs@yahoo.com.
Gobi VV
Research Scholar, Bharath University, Selaiyur, Chennai, Tamil Nadu, 600073, India.
Dhanalakshmi C
Department of Biochemistry and Biotechnology, Annamalai University, Annamalainagar, Tamil Nadu, 608002, India.
Manivasagam T
Department of Biochemistry and Biotechnology, Annamalai University, Annamalainagar, Tamil Nadu, 608002, India.
Justin Thenmozhi A
Department of Biochemistry and Biotechnology, Annamalai University, Annamalainagar, Tamil Nadu, 608002, India.
Essa MM
Department of Food Science and Nutrition, CAMS, Sultan Qaboos University, Muscat, Oman. Ageing and Dementia Research Group, Sultan Qaboos University, Muscat, Oman. Food and Brain Research Foundation, Chennai, Tamil Nadu, 600094, India.
Kalandar A
School of Medicine, College of Health and Medical Sciences, Haramaya University, Dire Dawa, Ethiopia.
Chidambaram R
Department of Radiology, Sri Lakshminarayana Institute of Medical Sciences, Puducherry, India.

MeSH

Cell DeathCell Line, TumorCell SurvivalCurcumaCurcuminCytochromes cDiarylheptanoidsDopaminergic NeuronsHumansInsecticidesMembrane Potential, MitochondrialNeuroprotective AgentsNeurotoxicity SyndromesOxidative StressParkinson DiseasePhytotherapyPlant ExtractsReactive Oxygen SpeciesRotenone

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28420370

Citation

Ramkumar, Muthu, et al. "Neuroprotective Effect of Demethoxycurcumin, a Natural Derivative of Curcumin On Rotenone Induced Neurotoxicity in SH-SY 5Y Neuroblastoma Cells." BMC Complementary and Alternative Medicine, vol. 17, no. 1, 2017, p. 217.
Ramkumar M, Rajasankar S, Gobi VV, et al. Neuroprotective effect of Demethoxycurcumin, a natural derivative of Curcumin on rotenone induced neurotoxicity in SH-SY 5Y Neuroblastoma cells. BMC Complement Altern Med. 2017;17(1):217.
Ramkumar, M., Rajasankar, S., Gobi, V. V., Dhanalakshmi, C., Manivasagam, T., Justin Thenmozhi, A., Essa, M. M., Kalandar, A., & Chidambaram, R. (2017). Neuroprotective effect of Demethoxycurcumin, a natural derivative of Curcumin on rotenone induced neurotoxicity in SH-SY 5Y Neuroblastoma cells. BMC Complementary and Alternative Medicine, 17(1), 217. https://doi.org/10.1186/s12906-017-1720-5
Ramkumar M, et al. Neuroprotective Effect of Demethoxycurcumin, a Natural Derivative of Curcumin On Rotenone Induced Neurotoxicity in SH-SY 5Y Neuroblastoma Cells. BMC Complement Altern Med. 2017 Apr 18;17(1):217. PubMed PMID: 28420370.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Neuroprotective effect of Demethoxycurcumin, a natural derivative of Curcumin on rotenone induced neurotoxicity in SH-SY 5Y Neuroblastoma cells. AU - Ramkumar,Muthu, AU - Rajasankar,Srinivasagam, AU - Gobi,Veerappan Venkatesh, AU - Dhanalakshmi,Chinnasamy, AU - Manivasagam,Thamilarasan, AU - Justin Thenmozhi,Arokiasamy, AU - Essa,Musthafa Mohamed, AU - Kalandar,Ameer, AU - Chidambaram,Ranganathan, Y1 - 2017/04/18/ PY - 2016/10/25/received PY - 2017/04/04/accepted PY - 2017/4/20/entrez PY - 2017/4/20/pubmed PY - 2017/8/2/medline KW - Apoptosis KW - Demethoxycurcumin KW - Neurodegenerative diseases KW - Oxidative stress KW - Rotenone SP - 217 EP - 217 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 17 IS - 1 N2 - BACKGROUND: Mitochondrial dysfunction and oxidative stress are the main toxic events leading to dopaminergic neuronal death in Parkinson's disease (PD) and identified as vital objective for therapeutic intercession. This study investigated the neuro-protective effects of the demethoxycurcumin (DMC), a derivative of curcumin against rotenone induced neurotoxicity. METHODS: SH-SY5Y neuroblastoma cells are divided into four experimental groups: untreated cells, cells incubated with rotenone (100 nM), cells treated with DMC (50 nM) + rotenone (100 nM) and DMC alone treated. 24 h after treatment with rotenone and 28 h after treatment with DMC, cell viability was assessed using the MTT assay, and levels of ROS and MMP, plus expression of apoptotic protein were analysed. RESULTS: Rotenone induced cell death in SH-SY5Y cells was significantly reduced by DMC pretreatment in a dose-dependent manner, indicating the potent neuroprotective effects of DMC. Rotenone treatment significantly increases the levels of ROS, loss of MMP, release of Cyt-c and expression of pro-apoptotic markers and decreases the expression of anti-apoptotic markers. CONCLUSIONS: Even though the results of the present study indicated that the DMC may serve as a potent therapeutic agent particularly for the treatment of neurodegenerative diseases like PD, further pre-clinical and clinical studies are required. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/28420370/Neuroprotective_effect_of_Demethoxycurcumin_a_natural_derivative_of_Curcumin_on_rotenone_induced_neurotoxicity_in_SH_SY_5Y_Neuroblastoma_cells_ L2 - https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/s12906-017-1720-5 DB - PRIME DP - Unbound Medicine ER -