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Increasing usage of rapid diagnostics for Dengue virus detection in Pakistan.

Abstract

OBJECTIVE

To evaluate the trends in usage of dengue virus diagnostics in Pakistan.

METHODS

This retrospective study was conducted at the Aga Khan University Hospital, Karachi, and comprised data for specimens tested for dengue virus from January 2012 to December 2015. Test for dengue virus ribonucleic acid by reverse transcription polymerase chain reaction, dengue virus antigen by immunochromatic assay and for human immunoglobulin M against dengue virus by enzyme-linked immunosorbent assay were reviewed. SPSS 17 was used for data analysis.

RESULTS

Overall, 33,577 specimens tested for dengue virus. Of them, 11,995 (35.7%) were positive. among them, 1,039(8.66%) were reported in 2012; 5,791(48.28%) in 2013; 1,027(8.56%) in 2014; and 4,138(34.49%) in 2015. In 2012, 966(93%) of the positive samples were diagnosed by immunoglobulin M-based method and 73(7%) by non-structural protein-1 antigen. In 2013, 4,401(76%) samples were tested positive by immunoglobulin M, 1,332(23%) by antigen and 58(1%) by polymerase chain reaction. The trend continued in 2014, but in 2015, 2,111(51%) of all dengue positive tests were determined by antigen testing, 1,969(47.6%) by immunoglobulin M and 58(1.4%) by polymerase chain reaction.

CONCLUSIONS

There was a shift in usage of direct virus identification for rapid diagnosis of dengue virus compared with host immunoglobulin M testing.

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  • Authors+Show Affiliations

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    The Aga Khan University, Karachi.

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    The Aga Khan University, Karachi.

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    The Aga Khan University, Karachi.

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    The Aga Khan University, Karachi.

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    The Aga Khan University, Karachi.

    ,

    The Aga Khan University, Karachi.

    The Aga Khan University, Karachi.

    Source

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    28420913