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Retinal and Circulating miRNAs in Age-Related Macular Degeneration: An In vivo Animal and Human Study.
Front Pharmacol. 2017; 8:168.FP

Abstract

Age related macular degeneration (AMD) is the leading cause of blindness among people aged 50 and over. Retinal deposition of amyloid-β (Aβ) aggregates in AMD patients has suggested a potential link between AMD and Alzheimer's disease (AD). We have evaluated the differential retinal expression profile of miRNAs in a rat model of AMD elicited by Aβ. A serum profile of miRNAs in AMD patients has been also assessed using single TaqMan assay. Analysis of retina from rats intravitreally injected with Aβ revealed that miR-27a, miR-146a, and miR-155 were up-regulated in comparison to control rats. Seven miRNA (miR-9, miR-23a, miR-27a, miR-34a, miR-126, miR-146a, and miR-155) have been found to be dysregulated in serum of AMD patients in comparison to control group. Analysis of pathways has revealed that dysregulated miRNAs, both in the AMD animal model and in AMD patients, can target genes regulating pathways linked to neurodegeneration and inflammation, reinforcing the hypothesis that AMD is a protein misfolding disease similar to AD. In fact, miR-9, miR-23a, miR-27a, miR-34a, miR-146a, miR-155 have been found to be dysregulated both in AMD and AD. In conclusion, we suggest that miR-9, miR-23a, miR-27a, miR-34a, miR-146a, miR-155 represent potential biomarkers and new pharmacological targets for AMD.

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Authors+Show Affiliations

Romano GL
Department of Biomedical and Biotechnological Sciences, Section of Pharmacology, University of CataniaCatania, Italy.
Platania CBM
Department of Biomedical and Biotechnological Sciences, Section of Pharmacology, University of CataniaCatania, Italy.
Drago F
Department of Biomedical and Biotechnological Sciences, Section of Pharmacology, University of CataniaCatania, Italy.
Salomone S
Department of Biomedical and Biotechnological Sciences, Section of Pharmacology, University of CataniaCatania, Italy.
Ragusa M
BioMolecular, Genome and Complex Systems BioMedicine Unit, Department of Biomedical and Biotechnological Sciences, Section of Biology and Genetics G. Sichel, University of CataniaCatania, Italy.
Barbagallo C
BioMolecular, Genome and Complex Systems BioMedicine Unit, Department of Biomedical and Biotechnological Sciences, Section of Biology and Genetics G. Sichel, University of CataniaCatania, Italy.
Di Pietro C
BioMolecular, Genome and Complex Systems BioMedicine Unit, Department of Biomedical and Biotechnological Sciences, Section of Biology and Genetics G. Sichel, University of CataniaCatania, Italy.
Purrello M
BioMolecular, Genome and Complex Systems BioMedicine Unit, Department of Biomedical and Biotechnological Sciences, Section of Biology and Genetics G. Sichel, University of CataniaCatania, Italy.
Reibaldi M
Department of Ophthalmology, School of Medicine, University of CataniaCatania, Italy.
Avitabile T
Department of Ophthalmology, School of Medicine, University of CataniaCatania, Italy.
Longo A
Department of Ophthalmology, School of Medicine, University of CataniaCatania, Italy.
Bucolo C
Department of Biomedical and Biotechnological Sciences, Section of Pharmacology, University of CataniaCatania, Italy.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28424619

Citation

Romano, Giovanni L., et al. "Retinal and Circulating miRNAs in Age-Related Macular Degeneration: an in Vivo Animal and Human Study." Frontiers in Pharmacology, vol. 8, 2017, p. 168.
Romano GL, Platania CBM, Drago F, et al. Retinal and Circulating miRNAs in Age-Related Macular Degeneration: An In vivo Animal and Human Study. Front Pharmacol. 2017;8:168.
Romano, G. L., Platania, C. B. M., Drago, F., Salomone, S., Ragusa, M., Barbagallo, C., Di Pietro, C., Purrello, M., Reibaldi, M., Avitabile, T., Longo, A., & Bucolo, C. (2017). Retinal and Circulating miRNAs in Age-Related Macular Degeneration: An In vivo Animal and Human Study. Frontiers in Pharmacology, 8, 168. https://doi.org/10.3389/fphar.2017.00168
Romano GL, et al. Retinal and Circulating miRNAs in Age-Related Macular Degeneration: an in Vivo Animal and Human Study. Front Pharmacol. 2017;8:168. PubMed PMID: 28424619.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Retinal and Circulating miRNAs in Age-Related Macular Degeneration: An In vivo Animal and Human Study. AU - Romano,Giovanni L, AU - Platania,Chiara B M, AU - Drago,Filippo, AU - Salomone,Salvatore, AU - Ragusa,Marco, AU - Barbagallo,Cristina, AU - Di Pietro,Cinzia, AU - Purrello,Michele, AU - Reibaldi,Michele, AU - Avitabile,Teresio, AU - Longo,Antonio, AU - Bucolo,Claudio, Y1 - 2017/03/30/ PY - 2017/01/28/received PY - 2017/03/14/accepted PY - 2017/4/21/entrez PY - 2017/4/21/pubmed PY - 2017/4/21/medline KW - Alzheimer's disease KW - age related macular degeneration KW - amyloid beta KW - miRNA KW - retinal diseases SP - 168 EP - 168 JF - Frontiers in pharmacology JO - Front Pharmacol VL - 8 N2 - Age related macular degeneration (AMD) is the leading cause of blindness among people aged 50 and over. Retinal deposition of amyloid-β (Aβ) aggregates in AMD patients has suggested a potential link between AMD and Alzheimer's disease (AD). We have evaluated the differential retinal expression profile of miRNAs in a rat model of AMD elicited by Aβ. A serum profile of miRNAs in AMD patients has been also assessed using single TaqMan assay. Analysis of retina from rats intravitreally injected with Aβ revealed that miR-27a, miR-146a, and miR-155 were up-regulated in comparison to control rats. Seven miRNA (miR-9, miR-23a, miR-27a, miR-34a, miR-126, miR-146a, and miR-155) have been found to be dysregulated in serum of AMD patients in comparison to control group. Analysis of pathways has revealed that dysregulated miRNAs, both in the AMD animal model and in AMD patients, can target genes regulating pathways linked to neurodegeneration and inflammation, reinforcing the hypothesis that AMD is a protein misfolding disease similar to AD. In fact, miR-9, miR-23a, miR-27a, miR-34a, miR-146a, miR-155 have been found to be dysregulated both in AMD and AD. In conclusion, we suggest that miR-9, miR-23a, miR-27a, miR-34a, miR-146a, miR-155 represent potential biomarkers and new pharmacological targets for AMD. SN - 1663-9812 UR - https://www.unboundmedicine.com/medline/citation/28424619/Retinal_and_Circulating_miRNAs_in_Age_Related_Macular_Degeneration:_An_In_vivo_Animal_and_Human_Study_ L2 - https://doi.org/10.3389/fphar.2017.00168 DB - PRIME DP - Unbound Medicine ER -