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Euphorbia dracunculoides L. abrogates carbon tetrachloride induced liver and DNA damage in rats.
BMC Complement Altern Med 2017; 17(1):223BC

Abstract

BACKGROUND

Evaluation of Euphorbia dracunculoides of family Euphorbiaceae during previous studies had established the in vitro antioxidant and in vivo anti-inflammatory activities. The plant is used by the local communities of Pakistan for various disorders including rheumatism and edema. In this investigation we have evaluated the hepatoprotective effects against CCl4 induced toxicity in rat.

METHODS

Dry powder of the aerial parts of E. dracunculoides was extracted with 95% methanol to get the extract (EDME). To investigate the hepatoprotective effects of EDME the Sprague-Dawley male rats were divided in to 8 groups with 6 rats in each. Group I and II were the normal and vehicle treated while the Groups III-VI were injected intraperitoneally with 1 ml of CCl4 (30% in olive oil). Rats of Group IV were orally administered with silymarin (50 mg/kg) while the Group V and VI with 200 mg/kg and 400 mg/kg of EDME, respectively. Animals of Group VII (200 mg/kg) and VIII (400 mg/kg) were treated with EDME alone. The treatments were given thrice a week for 4 weeks. Effects of EDME were evaluated for the protective effects against oxidative stress and genotoxicity induced with CCl4 in liver of rat.

RESULTS

Analysis of serum indicated significant (p < 0.05) rise in the level of aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP) and globulin whereas decrease was recorded for the total protein and albumin in CCl4 treated rats. In liver tissues the activity level of catalase (CAT), peroxidase (POD), superoxide dismutase (SOD), glutathione-S-transferase (GST), glutathione (GSH) was decreased while the level of lipid peroxides; thiobarbituric acid reactant substances (TBARS), nitrite and hydrogen peroxide increased in CCl4 treated rats as compared to the control group. Histopathological injuries and DNA damages were recorded in liver of rat with CCl4 treatment. However, co-administration of EDME, dose dependently, ameliorated the CCl4-induced hepatic toxicity in these parameters.

CONCLUSIONS

These results suggested that the phyto-constituents of EDME were able to ameliorate the oxidative stress induced with CCl4 and can be a useful therapeutic agent for oxidative stress related disorders.

Authors+Show Affiliations

Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, 45320, Pakistan.Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, 45320, Pakistan. mrkhanqau@yahoo.com.Department of Pharmacy, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, 45320, Pakistan.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28427398

Citation

Batool, Riffat, et al. "Euphorbia Dracunculoides L. Abrogates Carbon Tetrachloride Induced Liver and DNA Damage in Rats." BMC Complementary and Alternative Medicine, vol. 17, no. 1, 2017, p. 223.
Batool R, Khan MR, Majid M. Euphorbia dracunculoides L. abrogates carbon tetrachloride induced liver and DNA damage in rats. BMC Complement Altern Med. 2017;17(1):223.
Batool, R., Khan, M. R., & Majid, M. (2017). Euphorbia dracunculoides L. abrogates carbon tetrachloride induced liver and DNA damage in rats. BMC Complementary and Alternative Medicine, 17(1), p. 223. doi:10.1186/s12906-017-1744-x.
Batool R, Khan MR, Majid M. Euphorbia Dracunculoides L. Abrogates Carbon Tetrachloride Induced Liver and DNA Damage in Rats. BMC Complement Altern Med. 2017 Apr 20;17(1):223. PubMed PMID: 28427398.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Euphorbia dracunculoides L. abrogates carbon tetrachloride induced liver and DNA damage in rats. AU - Batool,Riffat, AU - Khan,Muhammad Rashid, AU - Majid,Muhammad, Y1 - 2017/04/20/ PY - 2016/12/15/received PY - 2017/04/13/accepted PY - 2017/4/22/entrez PY - 2017/4/22/pubmed PY - 2017/8/2/medline KW - Euphorbia Dracunculoides KW - Genotoxicity KW - Lipid peroxidation KW - Oxidative stress SP - 223 EP - 223 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 17 IS - 1 N2 - BACKGROUND: Evaluation of Euphorbia dracunculoides of family Euphorbiaceae during previous studies had established the in vitro antioxidant and in vivo anti-inflammatory activities. The plant is used by the local communities of Pakistan for various disorders including rheumatism and edema. In this investigation we have evaluated the hepatoprotective effects against CCl4 induced toxicity in rat. METHODS: Dry powder of the aerial parts of E. dracunculoides was extracted with 95% methanol to get the extract (EDME). To investigate the hepatoprotective effects of EDME the Sprague-Dawley male rats were divided in to 8 groups with 6 rats in each. Group I and II were the normal and vehicle treated while the Groups III-VI were injected intraperitoneally with 1 ml of CCl4 (30% in olive oil). Rats of Group IV were orally administered with silymarin (50 mg/kg) while the Group V and VI with 200 mg/kg and 400 mg/kg of EDME, respectively. Animals of Group VII (200 mg/kg) and VIII (400 mg/kg) were treated with EDME alone. The treatments were given thrice a week for 4 weeks. Effects of EDME were evaluated for the protective effects against oxidative stress and genotoxicity induced with CCl4 in liver of rat. RESULTS: Analysis of serum indicated significant (p < 0.05) rise in the level of aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP) and globulin whereas decrease was recorded for the total protein and albumin in CCl4 treated rats. In liver tissues the activity level of catalase (CAT), peroxidase (POD), superoxide dismutase (SOD), glutathione-S-transferase (GST), glutathione (GSH) was decreased while the level of lipid peroxides; thiobarbituric acid reactant substances (TBARS), nitrite and hydrogen peroxide increased in CCl4 treated rats as compared to the control group. Histopathological injuries and DNA damages were recorded in liver of rat with CCl4 treatment. However, co-administration of EDME, dose dependently, ameliorated the CCl4-induced hepatic toxicity in these parameters. CONCLUSIONS: These results suggested that the phyto-constituents of EDME were able to ameliorate the oxidative stress induced with CCl4 and can be a useful therapeutic agent for oxidative stress related disorders. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/28427398/Euphorbia_dracunculoides_L__abrogates_carbon_tetrachloride_induced_liver_and_DNA_damage_in_rats_ L2 - https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/s12906-017-1744-x DB - PRIME DP - Unbound Medicine ER -