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Saponins (Ginsenosides) from the Leaves of Panax quinquefolius Ameliorated Acetaminophen-Induced Hepatotoxicity in Mice.
J Agric Food Chem. 2017 May 10; 65(18):3684-3692.JA

Abstract

Acetaminophen (APAP) overdose is one of the most common inducements of drug-induced liver injury (DILI) in the world. The main purpose of this paper was to investigate the liver protection activity of saponins (ginsenosides) from the leaves of Panax quinquefolius (PQS) against APAP-induced hepatotoxicity, and the involved mechanisms were demonstrated for the first time. Mice were pretreated with PQS (150 and 300 mg/kg) by oral gavage for 7 days before being treated with 250 mg/kg APAP. Severe liver injury was exerted at 24 h post-APAP, and hepatotoxicity was assessed. Our results showed that pretreatment with PQS significantly decreased the serum alanine aminotransferase (ALT), aspartate transaminase (AST), tumor necrosis factor (TNF-α), and interleukin-1β (IL-1β) levels in a dose-dependent manner as compared to the APAP administration. Meanwhile, compared with that in the APAP group, PQS decreased hepatic malondialdehyde (MDA) contents and 4-hydroxynonenal (4-HNE) expression and restored reduced glutathione (GSH) content and superoxide dismutase (SOD) activity in livers of mice. PQS inhibited the overexpression of pro-inflammatory factors cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) in the liver tissues. Furthermore, Western blotting analysis revealed that PQS pretreatment inhibited the activation of apoptotic signaling pathways via increase of Bcl-2 and decrease of Bax and caspase-3 protein expression levels. Liver histopathological observation provided further evidence that PQS pretreatment significantly inhibited APAP-induced hepatocyte necrosis, inflammatory cell infiltration, and congestion. Biological indicators of nitrative stress such as 3-nitrotyrosine (3-NT) were inhibited after PQS pretreatment, compared to the APAP group. The present study clearly demonstrates that PQS exerts a protective effect against APAP-induced hepatic injury because of its antioxidant, anti-apoptotic, and anti-inflammatory activities. The findings from the present investigation show that PQS might be a promising candidate treatment agent against drug-induced ALI.

Authors+Show Affiliations

College of Chinese Medicinal Materials, Jilin Agricultural University , Changchun 130118, China.College of Chinese Medicinal Materials, Jilin Agricultural University , Changchun 130118, China.College of Chinese Medicinal Materials, Jilin Agricultural University , Changchun 130118, China.Institute of Special Wild Economic Animals and Plants, CAAS , Changchun 132109, China.Jilin Academy of Chinese Medicine Sciences , Changchun 130012, China.Institute of Special Wild Economic Animals and Plants, CAAS , Changchun 132109, China.Institute of Special Wild Economic Animals and Plants, CAAS , Changchun 132109, China.College of Chinese Medicinal Materials, Jilin Agricultural University , Changchun 130118, China.College of Chinese Medicinal Materials, Jilin Agricultural University , Changchun 130118, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28429935

Citation

Xu, Xing-Yue, et al. "Saponins (Ginsenosides) From the Leaves of Panax Quinquefolius Ameliorated Acetaminophen-Induced Hepatotoxicity in Mice." Journal of Agricultural and Food Chemistry, vol. 65, no. 18, 2017, pp. 3684-3692.
Xu XY, Hu JN, Liu Z, et al. Saponins (Ginsenosides) from the Leaves of Panax quinquefolius Ameliorated Acetaminophen-Induced Hepatotoxicity in Mice. J Agric Food Chem. 2017;65(18):3684-3692.
Xu, X. Y., Hu, J. N., Liu, Z., Zhang, R., He, Y. F., Hou, W., Wang, Z. Q., Yang, G., & Li, W. (2017). Saponins (Ginsenosides) from the Leaves of Panax quinquefolius Ameliorated Acetaminophen-Induced Hepatotoxicity in Mice. Journal of Agricultural and Food Chemistry, 65(18), 3684-3692. https://doi.org/10.1021/acs.jafc.7b00610
Xu XY, et al. Saponins (Ginsenosides) From the Leaves of Panax Quinquefolius Ameliorated Acetaminophen-Induced Hepatotoxicity in Mice. J Agric Food Chem. 2017 May 10;65(18):3684-3692. PubMed PMID: 28429935.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Saponins (Ginsenosides) from the Leaves of Panax quinquefolius Ameliorated Acetaminophen-Induced Hepatotoxicity in Mice. AU - Xu,Xing-Yue, AU - Hu,Jun-Nan, AU - Liu,Zhi, AU - Zhang,Rui, AU - He,Yu-Fang, AU - Hou,Wei, AU - Wang,Zhi-Qing, AU - Yang,Ge, AU - Li,Wei, Y1 - 2017/04/25/ PY - 2017/4/22/pubmed PY - 2017/6/9/medline PY - 2017/4/22/entrez KW - APAP-induced liver injury KW - anti-apoptosis KW - anti-inflammation KW - leaves of Panax quinquefolius KW - oxidative stress KW - saponins SP - 3684 EP - 3692 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 65 IS - 18 N2 - Acetaminophen (APAP) overdose is one of the most common inducements of drug-induced liver injury (DILI) in the world. The main purpose of this paper was to investigate the liver protection activity of saponins (ginsenosides) from the leaves of Panax quinquefolius (PQS) against APAP-induced hepatotoxicity, and the involved mechanisms were demonstrated for the first time. Mice were pretreated with PQS (150 and 300 mg/kg) by oral gavage for 7 days before being treated with 250 mg/kg APAP. Severe liver injury was exerted at 24 h post-APAP, and hepatotoxicity was assessed. Our results showed that pretreatment with PQS significantly decreased the serum alanine aminotransferase (ALT), aspartate transaminase (AST), tumor necrosis factor (TNF-α), and interleukin-1β (IL-1β) levels in a dose-dependent manner as compared to the APAP administration. Meanwhile, compared with that in the APAP group, PQS decreased hepatic malondialdehyde (MDA) contents and 4-hydroxynonenal (4-HNE) expression and restored reduced glutathione (GSH) content and superoxide dismutase (SOD) activity in livers of mice. PQS inhibited the overexpression of pro-inflammatory factors cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) in the liver tissues. Furthermore, Western blotting analysis revealed that PQS pretreatment inhibited the activation of apoptotic signaling pathways via increase of Bcl-2 and decrease of Bax and caspase-3 protein expression levels. Liver histopathological observation provided further evidence that PQS pretreatment significantly inhibited APAP-induced hepatocyte necrosis, inflammatory cell infiltration, and congestion. Biological indicators of nitrative stress such as 3-nitrotyrosine (3-NT) were inhibited after PQS pretreatment, compared to the APAP group. The present study clearly demonstrates that PQS exerts a protective effect against APAP-induced hepatic injury because of its antioxidant, anti-apoptotic, and anti-inflammatory activities. The findings from the present investigation show that PQS might be a promising candidate treatment agent against drug-induced ALI. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/28429935/Saponins__Ginsenosides__from_the_Leaves_of_Panax_quinquefolius_Ameliorated_Acetaminophen_Induced_Hepatotoxicity_in_Mice_ L2 - https://doi.org/10.1021/acs.jafc.7b00610 DB - PRIME DP - Unbound Medicine ER -