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Genotyping-by-sequencing targeting of a novel downy mildew resistance gene Pl 20 from wild Helianthus argophyllus for sunflower (Helianthus annuus L.).
Theor Appl Genet. 2017 Jul; 130(7):1519-1529.TA

Abstract

KEY MESSAGE

Genotyping-by-sequencing revealed a new downy mildew resistance gene, Pl 20 , from wild Helianthus argophyllus located on linkage group 8 of the sunflower genome and closely linked to SNP markers that facilitate the marker-assisted selection of resistance genes. Downy mildew (DM), caused by Plasmopara halstedii, is one of the most devastating and yield-limiting diseases of sunflower. Downy mildew resistance identified in wild Helianthus argophyllus accession PI 494578 was determined to be effective against the predominant and virulent races of P. halstedii occurring in the United States. The evaluation of 114 BC1F2:3 families derived from the cross between HA 89 and PI 494578 against P. halstedii race 734 revealed that single dominant gene controls downy mildew resistance in the population. Genotyping-by-sequencing analysis conducted in the BC1F2 population indicated that the DM resistance gene derived from wild H. argophyllus PI 494578 is located on the upper end of the linkage group (LG) 8 of the sunflower genome, as was determined single nucleotide polymorphism (SNP) markers associated with DM resistance. Analysis of 11 additional SNP markers previously mapped to this region revealed that the resistance gene, named Pl 20 , co-segregated with four markers, SFW02745, SFW09076, S8_11272025, and S8_11272046, and is flanked by SFW04358 and S8_100385559 at an interval of 1.8 cM. The newly discovered P. halstedii resistance gene has been introgressed from wild species into cultivated sunflower to provide a novel gene with DM resistance. The homozygous resistant individuals were selected from BC2F2 progenies with the use of markers linked to the Pl 20 gene, and these lines should benefit the sunflower community for Helianthus improvement.

Authors+Show Affiliations

Department of Plant Pathology, North Dakota State University, Fargo, ND, 58108, USA.Department of Plant Pathology, North Dakota State University, Fargo, ND, 58108, USA.Soybean Genomics and Improvement Lab, USDA-Agricultural Research Service, Beltsville, MD, 20705-2350, USA.Red River Valley Agricultural Research Center, USDA-Agricultural Research Service, 1605 Albrecht Blvd N, Fargo, ND, 58102-2765, USA. lili.qi@ars.usda.gov.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28432412

Citation

Ma, G J., et al. "Genotyping-by-sequencing Targeting of a Novel Downy Mildew Resistance Gene Pl 20 From Wild Helianthus Argophyllus for Sunflower (Helianthus Annuus L.)." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 130, no. 7, 2017, pp. 1519-1529.
Ma GJ, Markell SG, Song QJ, et al. Genotyping-by-sequencing targeting of a novel downy mildew resistance gene Pl 20 from wild Helianthus argophyllus for sunflower (Helianthus annuus L.). Theor Appl Genet. 2017;130(7):1519-1529.
Ma, G. J., Markell, S. G., Song, Q. J., & Qi, L. L. (2017). Genotyping-by-sequencing targeting of a novel downy mildew resistance gene Pl 20 from wild Helianthus argophyllus for sunflower (Helianthus annuus L.). TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 130(7), 1519-1529. https://doi.org/10.1007/s00122-017-2906-4
Ma GJ, et al. Genotyping-by-sequencing Targeting of a Novel Downy Mildew Resistance Gene Pl 20 From Wild Helianthus Argophyllus for Sunflower (Helianthus Annuus L.). Theor Appl Genet. 2017;130(7):1519-1529. PubMed PMID: 28432412.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genotyping-by-sequencing targeting of a novel downy mildew resistance gene Pl 20 from wild Helianthus argophyllus for sunflower (Helianthus annuus L.). AU - Ma,G J, AU - Markell,S G, AU - Song,Q J, AU - Qi,L L, Y1 - 2017/04/21/ PY - 2017/02/28/received PY - 2017/04/07/accepted PY - 2017/4/23/pubmed PY - 2017/6/18/medline PY - 2017/4/23/entrez SP - 1519 EP - 1529 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor. Appl. Genet. VL - 130 IS - 7 N2 - KEY MESSAGE: Genotyping-by-sequencing revealed a new downy mildew resistance gene, Pl 20 , from wild Helianthus argophyllus located on linkage group 8 of the sunflower genome and closely linked to SNP markers that facilitate the marker-assisted selection of resistance genes. Downy mildew (DM), caused by Plasmopara halstedii, is one of the most devastating and yield-limiting diseases of sunflower. Downy mildew resistance identified in wild Helianthus argophyllus accession PI 494578 was determined to be effective against the predominant and virulent races of P. halstedii occurring in the United States. The evaluation of 114 BC1F2:3 families derived from the cross between HA 89 and PI 494578 against P. halstedii race 734 revealed that single dominant gene controls downy mildew resistance in the population. Genotyping-by-sequencing analysis conducted in the BC1F2 population indicated that the DM resistance gene derived from wild H. argophyllus PI 494578 is located on the upper end of the linkage group (LG) 8 of the sunflower genome, as was determined single nucleotide polymorphism (SNP) markers associated with DM resistance. Analysis of 11 additional SNP markers previously mapped to this region revealed that the resistance gene, named Pl 20 , co-segregated with four markers, SFW02745, SFW09076, S8_11272025, and S8_11272046, and is flanked by SFW04358 and S8_100385559 at an interval of 1.8 cM. The newly discovered P. halstedii resistance gene has been introgressed from wild species into cultivated sunflower to provide a novel gene with DM resistance. The homozygous resistant individuals were selected from BC2F2 progenies with the use of markers linked to the Pl 20 gene, and these lines should benefit the sunflower community for Helianthus improvement. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/28432412/Genotyping_by_sequencing_targeting_of_a_novel_downy_mildew_resistance_gene_Pl_20_from_wild_Helianthus_argophyllus_for_sunflower__Helianthus_annuus_L___ L2 - https://dx.doi.org/10.1007/s00122-017-2906-4 DB - PRIME DP - Unbound Medicine ER -