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Genetic dissection of the planarian reproductive system through characterization of Schmidtea mediterranea CPEB homologs.
Dev Biol 2017; 426(1):43-55DB

Abstract

Cytoplasmic polyadenylation is a mechanism of mRNA regulation prevalent in metazoan germ cells; it is largely dependent on Cytoplasmic Polyadenylation Element Binding proteins (CPEBs). Two CPEB homologs were identified in the planarian Schmidtea mediterranea. Smed-CPEB1 is expressed in ovaries and yolk glands of sexually mature planarians, and required for oocyte and yolk gland development. In contrast, Smed-CPEB2 is expressed in the testes and the central nervous system; its function is required for spermatogenesis as well as non-autonomously for development of ovaries and accessory reproductive organs. Transcriptome analysis of CPEB knockdown animals uncovered a comprehensive collection of molecular markers for reproductive structures in S. mediterranea, including ovaries, testes, yolk glands, and the copulatory apparatus. Analysis by RNA interference revealed contributions for a dozen of these genes during oogenesis, spermatogenesis, or capsule formation. We also present evidence suggesting that Smed-CPEB2 promotes translation of Neuropeptide Y-8, a prohormone required for planarian sexual maturation. These findings provide mechanistic insight into potentially conserved processes of germ cell development, as well as events involved in capsule deposition by flatworms.

Authors+Show Affiliations

Department of Biological Sciences, Wright State University, 3640 Colonel Glenn Highway, Dayton, OH 45435, USA; Howard Hughes Medical Institute and Department of Cell and Developmental Biology, University of Illinois at Urbana-Champaign, 601 S. Goodwin Ave., Urbana, IL 61801, USA. Electronic address: labib.rouhana@wright.edu.Department of Biological Sciences, Wright State University, 3640 Colonel Glenn Highway, Dayton, OH 45435, USA.Howard Hughes Medical Institute and Department of Cell and Developmental Biology, University of Illinois at Urbana-Champaign, 601 S. Goodwin Ave., Urbana, IL 61801, USA.Howard Hughes Medical Institute and Department of Cell and Developmental Biology, University of Illinois at Urbana-Champaign, 601 S. Goodwin Ave., Urbana, IL 61801, USA.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

28434803

Citation

Rouhana, Labib, et al. "Genetic Dissection of the Planarian Reproductive System Through Characterization of Schmidtea Mediterranea CPEB Homologs." Developmental Biology, vol. 426, no. 1, 2017, pp. 43-55.
Rouhana L, Tasaki J, Saberi A, et al. Genetic dissection of the planarian reproductive system through characterization of Schmidtea mediterranea CPEB homologs. Dev Biol. 2017;426(1):43-55.
Rouhana, L., Tasaki, J., Saberi, A., & Newmark, P. A. (2017). Genetic dissection of the planarian reproductive system through characterization of Schmidtea mediterranea CPEB homologs. Developmental Biology, 426(1), pp. 43-55. doi:10.1016/j.ydbio.2017.04.008.
Rouhana L, et al. Genetic Dissection of the Planarian Reproductive System Through Characterization of Schmidtea Mediterranea CPEB Homologs. Dev Biol. 2017 06 1;426(1):43-55. PubMed PMID: 28434803.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic dissection of the planarian reproductive system through characterization of Schmidtea mediterranea CPEB homologs. AU - Rouhana,Labib, AU - Tasaki,Junichi, AU - Saberi,Amir, AU - Newmark,Phillip A, Y1 - 2017/04/21/ PY - 2017/01/06/received PY - 2017/03/30/revised PY - 2017/04/18/accepted PY - 2017/4/25/pubmed PY - 2017/9/20/medline PY - 2017/4/25/entrez KW - CPEB KW - Ectolecithal reproduction KW - Oogenesis KW - Platyhelminthes KW - Spermatogenesis KW - Vitellaria SP - 43 EP - 55 JF - Developmental biology JO - Dev. Biol. VL - 426 IS - 1 N2 - Cytoplasmic polyadenylation is a mechanism of mRNA regulation prevalent in metazoan germ cells; it is largely dependent on Cytoplasmic Polyadenylation Element Binding proteins (CPEBs). Two CPEB homologs were identified in the planarian Schmidtea mediterranea. Smed-CPEB1 is expressed in ovaries and yolk glands of sexually mature planarians, and required for oocyte and yolk gland development. In contrast, Smed-CPEB2 is expressed in the testes and the central nervous system; its function is required for spermatogenesis as well as non-autonomously for development of ovaries and accessory reproductive organs. Transcriptome analysis of CPEB knockdown animals uncovered a comprehensive collection of molecular markers for reproductive structures in S. mediterranea, including ovaries, testes, yolk glands, and the copulatory apparatus. Analysis by RNA interference revealed contributions for a dozen of these genes during oogenesis, spermatogenesis, or capsule formation. We also present evidence suggesting that Smed-CPEB2 promotes translation of Neuropeptide Y-8, a prohormone required for planarian sexual maturation. These findings provide mechanistic insight into potentially conserved processes of germ cell development, as well as events involved in capsule deposition by flatworms. SN - 1095-564X UR - https://www.unboundmedicine.com/medline/citation/28434803/Genetic_dissection_of_the_planarian_reproductive_system_through_characterization_of_Schmidtea_mediterranea_CPEB_homologs_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0012-1606(17)30019-2 DB - PRIME DP - Unbound Medicine ER -