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A novel biosensor based on boronic acid functionalized metal-organic frameworks for the determination of hydrogen peroxide released from living cells.
Biosens Bioelectron. 2017 Sep 15; 95:131-137.BB

Abstract

In this work, we report a durable and sensitive H2O2 biosensor based on boronic acid functionalized metal-organic frameworks (denoted as MIL-100(Cr)-B) as an efficient immobilization matrix of horseradish peroxidase (HRP). MIL-100(Cr)-B features a hierarchical porous structure, extremely high surface area, and sufficient recognition sites, which can significantly increase HRP loading and prevent them from leakage and deactivation. The H2O2 biosensor can be easily achieved without any complex processing. Meanwhile, the immobilized HRP exhibited enhanced stability and remarkable catalytic activity towards H2O2 reduction. Under optimal conditions, the biosensor showed a fast response time (less than 4s) to H2O2 in a wide linear range of 0.5-3000μM with a low detection limit of 0.1μM, as well as good anti-interference ability and long-term storage stability. These excellent performances substantially enable the proposed biosensor to be used for the real-time detection of H2O2 released from living cells with satisfactory results, thus showing the potential application in the study of H2O2-involved dynamic pathological and physiological process.

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Authors+Show Affiliations

State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China; Department of Chemistry, Lanzhou University, Lanzhou 730000, China; Gansu University of Chinese Medicine, Lanzhou 730000, China.

State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China; Department of Chemistry, Lanzhou University, Lanzhou 730000, China. Electronic address: lvwenj@lzu.edu.cn.

State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China; Department of Chemistry, Lanzhou University, Lanzhou 730000, China.

State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China; Department of Chemistry, Lanzhou University, Lanzhou 730000, China.

State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China; Department of Chemistry, Lanzhou University, Lanzhou 730000, China.

State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China; Department of Chemistry, Lanzhou University, Lanzhou 730000, China.

State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China; Department of Chemistry, Lanzhou University, Lanzhou 730000, China.

State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China; Department of Chemistry, Lanzhou University, Lanzhou 730000, China.

State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China; Department of Chemistry, Lanzhou University, Lanzhou 730000, China; Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, Lanzhou 730000, China. Electronic address: chenxg@lzu.edu.cn.

MeSH

Biosensing TechniquesBoronic AcidsElectrochemical TechniquesEnzymes, ImmobilizedHorseradish PeroxidaseHydrogen PeroxideLimit of DetectionMetal NanoparticlesMetal-Organic Frameworks

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28437639

Citation

Dai, Hongxia, et al. "A Novel Biosensor Based On Boronic Acid Functionalized Metal-organic Frameworks for the Determination of Hydrogen Peroxide Released From Living Cells." Biosensors & Bioelectronics, vol. 95, 2017, pp. 131-137.

Dai H, Lü W, Zuo X, et al. A novel biosensor based on boronic acid functionalized metal-organic frameworks for the determination of hydrogen peroxide released from living cells. Biosens Bioelectron. 2017;95:131-137.

Dai, H., Lü, W., Zuo, X., Zhu, Q., Pan, C., Niu, X., Liu, J., Chen, H., & Chen, X. (2017). A novel biosensor based on boronic acid functionalized metal-organic frameworks for the determination of hydrogen peroxide released from living cells. Biosensors & Bioelectronics, 95, 131-137. https://doi.org/10.1016/j.bios.2017.04.021

Dai H, et al. A Novel Biosensor Based On Boronic Acid Functionalized Metal-organic Frameworks for the Determination of Hydrogen Peroxide Released From Living Cells. Biosens Bioelectron. 2017 Sep 15;95:131-137. PubMed PMID: 28437639.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - A novel biosensor based on boronic acid functionalized metal-organic frameworks for the determination of hydrogen peroxide released from living cells. AU - Dai,Hongxia, AU - Lü,Wenjuan, AU - Zuo,Xianwei, AU - Zhu,Qian, AU - Pan,Congjie, AU - Niu,Xiaoying, AU - Liu,Juanjuan, AU - Chen,HongLi, AU - Chen,Xingguo, Y1 - 2017/04/18/ PY - 2017/01/12/received PY - 2017/03/23/revised PY - 2017/04/17/accepted PY - 2017/4/25/pubmed PY - 2018/2/15/medline PY - 2017/4/25/entrez KW - Boronic acid KW - Electrochemical biosensor KW - Horseradish peroxidase KW - Hydrogen peroxide KW - Metal-organic frameworks SP - 131 EP - 137 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 95 N2 - In this work, we report a durable and sensitive H2O2 biosensor based on boronic acid functionalized metal-organic frameworks (denoted as MIL-100(Cr)-B) as an efficient immobilization matrix of horseradish peroxidase (HRP). MIL-100(Cr)-B features a hierarchical porous structure, extremely high surface area, and sufficient recognition sites, which can significantly increase HRP loading and prevent them from leakage and deactivation. The H2O2 biosensor can be easily achieved without any complex processing. Meanwhile, the immobilized HRP exhibited enhanced stability and remarkable catalytic activity towards H2O2 reduction. Under optimal conditions, the biosensor showed a fast response time (less than 4s) to H2O2 in a wide linear range of 0.5-3000μM with a low detection limit of 0.1μM, as well as good anti-interference ability and long-term storage stability. These excellent performances substantially enable the proposed biosensor to be used for the real-time detection of H2O2 released from living cells with satisfactory results, thus showing the potential application in the study of H2O2-involved dynamic pathological and physiological process. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/28437639/A_novel_biosensor_based_on_boronic_acid_functionalized_metal_organic_frameworks_for_the_determination_of_hydrogen_peroxide_released_from_living_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(17)30269-5 DB - PRIME DP - Unbound Medicine ER -