Tags

Type your tag names separated by a space and hit enter

PirAB protein from Xenorhabdus nematophila HB310 exhibits a binary toxin with insecticidal activity and cytotoxicity in Galleria mellonella.
J Invertebr Pathol. 2017 09; 148:43-50.JI

Abstract

PirAB (Photorhabdus insect-related proteins, PirAB) toxin was initially found in the Photorhabdus luminescens TT01 strain and has been shown to be a binary toxin with high insecticidal activity. Based on GenBank data, this gene was also found in the Xenorhabdus nematophila genome sequence. The predicted amino acid sequence of pirA and pirB in the genome of X. nematophila showed 51% and 50% identity with those gene sequences from P. luminescens. The purpose of this experiment is to identify the relevant information for this toxin gene in X. nematophila. The pirA, pirB and pirAB genes of X. nematophila HB310 were cloned and expressed in Escherichia coli BL21 (DE3) using the pET-28a vector. A PirAB-fusion protein (PirAB-F) was constructed by linking the pirA and pirB genes with the flexible linker (Gly)4 DNA encoding sequence and then efficiently expressed in E. coli. The hemocoel and oral insecticidal activities of the recombinant proteins were analyzed against the larvae of Galleria mellonella. The results show that PirA/B alone, PirA/B mixture, co-expressed PirAB protein, and PirAB-F all had no oral insecticidal activity against the second-instar larvae of G. mellonella. Only PirA/B mixture and co-expressed PirAB protein had hemocoel insecticidal activity against G. mellonella fifth-instar larvae, with an LD50 of 2.718μg/larva or 1.566μg/larva, respectively. Therefore, we confirmed that PirAB protein of X. nematophila HB310 is a binary insecticidal toxin. The successful expression and purification of PirAB laid a foundation for further studies on the function, insecticidal mechanism and expression regulation of the binary toxin.

Authors+Show Affiliations

College of Plant Protection, Hebei Agricultural University, 071001 Baoding, Hebei Province, China.Luanping State-owned Forestry Farm Management of Chengde City, 068250 Chengde, China.College of Plant Protection, Hebei Agricultural University, 071001 Baoding, Hebei Province, China.College of Plant Protection, Hebei Agricultural University, 071001 Baoding, Hebei Province, China.College of Plant Protection, Hebei Agricultural University, 071001 Baoding, Hebei Province, China.College of Plant Protection, Hebei Agricultural University, 071001 Baoding, Hebei Province, China. Electronic address: ngzyheb@163.com.College of Plant Protection, Hebei Agricultural University, 071001 Baoding, Hebei Province, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

28438456

Citation

Yang, Qing, et al. "PirAB Protein From Xenorhabdus Nematophila HB310 Exhibits a Binary Toxin With Insecticidal Activity and Cytotoxicity in Galleria Mellonella." Journal of Invertebrate Pathology, vol. 148, 2017, pp. 43-50.
Yang Q, Zhang J, Li T, et al. PirAB protein from Xenorhabdus nematophila HB310 exhibits a binary toxin with insecticidal activity and cytotoxicity in Galleria mellonella. J Invertebr Pathol. 2017;148:43-50.
Yang, Q., Zhang, J., Li, T., Liu, S., Song, P., Nangong, Z., & Wang, Q. (2017). PirAB protein from Xenorhabdus nematophila HB310 exhibits a binary toxin with insecticidal activity and cytotoxicity in Galleria mellonella. Journal of Invertebrate Pathology, 148, 43-50. https://doi.org/10.1016/j.jip.2017.04.007
Yang Q, et al. PirAB Protein From Xenorhabdus Nematophila HB310 Exhibits a Binary Toxin With Insecticidal Activity and Cytotoxicity in Galleria Mellonella. J Invertebr Pathol. 2017;148:43-50. PubMed PMID: 28438456.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - PirAB protein from Xenorhabdus nematophila HB310 exhibits a binary toxin with insecticidal activity and cytotoxicity in Galleria mellonella. AU - Yang,Qing, AU - Zhang,Jie, AU - Li,Tianhui, AU - Liu,Shen, AU - Song,Ping, AU - Nangong,Ziyan, AU - Wang,Qinying, Y1 - 2017/04/21/ PY - 2016/12/19/received PY - 2017/04/05/revised PY - 2017/04/20/accepted PY - 2017/4/26/pubmed PY - 2018/5/16/medline PY - 2017/4/26/entrez KW - Binary toxin KW - Galleria mellonella KW - Insecticidal activity KW - PirAB protein KW - Xenorhabdus nematophila SP - 43 EP - 50 JF - Journal of invertebrate pathology JO - J Invertebr Pathol VL - 148 N2 - PirAB (Photorhabdus insect-related proteins, PirAB) toxin was initially found in the Photorhabdus luminescens TT01 strain and has been shown to be a binary toxin with high insecticidal activity. Based on GenBank data, this gene was also found in the Xenorhabdus nematophila genome sequence. The predicted amino acid sequence of pirA and pirB in the genome of X. nematophila showed 51% and 50% identity with those gene sequences from P. luminescens. The purpose of this experiment is to identify the relevant information for this toxin gene in X. nematophila. The pirA, pirB and pirAB genes of X. nematophila HB310 were cloned and expressed in Escherichia coli BL21 (DE3) using the pET-28a vector. A PirAB-fusion protein (PirAB-F) was constructed by linking the pirA and pirB genes with the flexible linker (Gly)4 DNA encoding sequence and then efficiently expressed in E. coli. The hemocoel and oral insecticidal activities of the recombinant proteins were analyzed against the larvae of Galleria mellonella. The results show that PirA/B alone, PirA/B mixture, co-expressed PirAB protein, and PirAB-F all had no oral insecticidal activity against the second-instar larvae of G. mellonella. Only PirA/B mixture and co-expressed PirAB protein had hemocoel insecticidal activity against G. mellonella fifth-instar larvae, with an LD50 of 2.718μg/larva or 1.566μg/larva, respectively. Therefore, we confirmed that PirAB protein of X. nematophila HB310 is a binary insecticidal toxin. The successful expression and purification of PirAB laid a foundation for further studies on the function, insecticidal mechanism and expression regulation of the binary toxin. SN - 1096-0805 UR - https://www.unboundmedicine.com/medline/citation/28438456/PirAB_protein_from_Xenorhabdus_nematophila_HB310_exhibits_a_binary_toxin_with_insecticidal_activity_and_cytotoxicity_in_Galleria_mellonella_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-2011(16)30257-9 DB - PRIME DP - Unbound Medicine ER -