Tags

Type your tag names separated by a space and hit enter

The specific killing effect of matrine on castration-resistant prostate cancer cells by targeting the Akt/FoxO3a signaling pathway.
Oncol Rep. 2017 May; 37(5):2819-2828.OR

Abstract

Matrine, a Sophora alkaloid, exhibits antiproliferative and anti-carcinogenic activities through several mechanisms. In a previous study, we found that matrine could effectively inhibit the proliferation of castration-resistant prostate cancer (CRPC). In the present study, the effect of matrine and LY294002 on the expression of the Akt/FoxO3a signaling pathway was examined by western blot analyses and RT-PCR. We discovered that matrine significantly inhibited the proliferation of both prostate cancer cell line PC-3 and prostate epithelial cell line RWPE1, induced apoptosis and induced cell cycle arrest. In addition, LY294002 was found to enhance the effect of matrine. Furthermore, the effects of matrine on the inhibition of proliferation and the induction of cell cycle arrest and cell apoptosis were more effective on PC-3 than on RWPE1 cells. Compared to RWPE1 cells, matrine exerted a more powerful influence on PC-3 cells in increasing the expression of the relevant protein. Our data suggested that FoxO3a-Bim and FoxO3a-P27 may mediate matrine-inhibited proliferation of CRPC cells by activating cell apoptosis and inducing cell cycle arrest. Matrine exhibited high selectivity in killing CRPC cells. Our findings demonstrated that matrine could be used in a potential therapeutic role in the management of CRPC in humans.

Authors+Show Affiliations

Department of Radiation Oncology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, P.R. China.Department of Radiation Oncology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, P.R. China.Department of Radiation Oncology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, P.R. China.Department of Radiation Oncology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, P.R. China.Department of Urology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, P.R. China.Department of Urology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, P.R. China.Department of Urology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, P.R. China.Department of Urology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, P.R. China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28440481

Citation

Bai, Shoumin, et al. "The Specific Killing Effect of Matrine On Castration-resistant Prostate Cancer Cells By Targeting the Akt/FoxO3a Signaling Pathway." Oncology Reports, vol. 37, no. 5, 2017, pp. 2819-2828.
Bai S, Chen T, Yu X, et al. The specific killing effect of matrine on castration-resistant prostate cancer cells by targeting the Akt/FoxO3a signaling pathway. Oncol Rep. 2017;37(5):2819-2828.
Bai, S., Chen, T., Yu, X., Luo, M., Chen, X., Lin, C., Lai, Y., & Huang, H. (2017). The specific killing effect of matrine on castration-resistant prostate cancer cells by targeting the Akt/FoxO3a signaling pathway. Oncology Reports, 37(5), 2819-2828. https://doi.org/10.3892/or.2017.5510
Bai S, et al. The Specific Killing Effect of Matrine On Castration-resistant Prostate Cancer Cells By Targeting the Akt/FoxO3a Signaling Pathway. Oncol Rep. 2017;37(5):2819-2828. PubMed PMID: 28440481.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The specific killing effect of matrine on castration-resistant prostate cancer cells by targeting the Akt/FoxO3a signaling pathway. AU - Bai,Shoumin, AU - Chen,Ting, AU - Yu,Xiaoli, AU - Luo,Ming, AU - Chen,Xianju, AU - Lin,Chunhao, AU - Lai,Yiming, AU - Huang,Hai, Y1 - 2017/03/16/ PY - 2016/12/01/received PY - 2017/01/26/accepted PY - 2017/4/26/entrez PY - 2017/4/26/pubmed PY - 2018/2/8/medline SP - 2819 EP - 2828 JF - Oncology reports JO - Oncol Rep VL - 37 IS - 5 N2 - Matrine, a Sophora alkaloid, exhibits antiproliferative and anti-carcinogenic activities through several mechanisms. In a previous study, we found that matrine could effectively inhibit the proliferation of castration-resistant prostate cancer (CRPC). In the present study, the effect of matrine and LY294002 on the expression of the Akt/FoxO3a signaling pathway was examined by western blot analyses and RT-PCR. We discovered that matrine significantly inhibited the proliferation of both prostate cancer cell line PC-3 and prostate epithelial cell line RWPE1, induced apoptosis and induced cell cycle arrest. In addition, LY294002 was found to enhance the effect of matrine. Furthermore, the effects of matrine on the inhibition of proliferation and the induction of cell cycle arrest and cell apoptosis were more effective on PC-3 than on RWPE1 cells. Compared to RWPE1 cells, matrine exerted a more powerful influence on PC-3 cells in increasing the expression of the relevant protein. Our data suggested that FoxO3a-Bim and FoxO3a-P27 may mediate matrine-inhibited proliferation of CRPC cells by activating cell apoptosis and inducing cell cycle arrest. Matrine exhibited high selectivity in killing CRPC cells. Our findings demonstrated that matrine could be used in a potential therapeutic role in the management of CRPC in humans. SN - 1791-2431 UR - https://www.unboundmedicine.com/medline/citation/28440481/The_specific_killing_effect_of_matrine_on_castration_resistant_prostate_cancer_cells_by_targeting_the_Akt/FoxO3a_signaling_pathway_ L2 - https://www.spandidos-publications.com/or/37/5/2819 DB - PRIME DP - Unbound Medicine ER -