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Fluorescent Probes and Selective Inhibitors for Biological Studies of Hydrogen Sulfide- and Polysulfide-Mediated Signaling.
Antioxid Redox Signal. 2017 Oct 01; 27(10):669-683.AR

Abstract

SIGNIFICANCE

Hydrogen sulfide (H2S) plays roles in many physiological processes, including relaxation of vascular smooth muscles, mediation of neurotransmission, inhibition of insulin signaling, and regulation of inflammation. Also, hydropersulfide (R-S-SH) and polysulfide (-S-Sn-S-) have recently been identified as reactive sulfur species (RSS) that regulate the bioactivities of multiple proteins via S-sulfhydration of cysteine residues (protein Cys-SSH) and show cytoprotection. Chemical tools such as fluorescent probes and selective inhibitors are needed to establish in detail the physiological roles of H2S and polysulfide. Recent Advances: Although many fluorescent probes for H2S are available, fluorescent probes for hydropersulfide and polysulfide have only recently been developed and used to detect these sulfur species in living cells.

CRITICAL ISSUES

In this review, we summarize recent progress in developing chemical tools for the study of H2S, hydropersulfide, and polysulfide, covering fluorescent probes based on various design strategies and selective inhibitors of H2S- and polysulfide-producing enzymes (cystathionine γ-lyase, cystathionine β-synthase, and 3-mercaptopyruvate sulfurtransferase), and we summarize their applications in biological studies.

FUTURE DIRECTIONS

Despite recent progress, the precise biological functions of H2S, hydropersulfide, and polysulfide remain to be fully established. Fluorescent probes and selective inhibitors are effective chemical tools to study the physiological roles of these sulfur molecules in living cells and tissues. Therefore, further development of a broad range of practical fluorescent probes and selective inhibitors as tools for studies of RSS biology is currently attracting great interest. Antioxid. Redox Signal. 27, 669-683.

Authors+Show Affiliations

Graduate School of Pharmaceutical Sciences, The University of Tokyo , Tokyo, Japan .Graduate School of Pharmaceutical Sciences, The University of Tokyo , Tokyo, Japan .Graduate School of Pharmaceutical Sciences, The University of Tokyo , Tokyo, Japan .

Pub Type(s)

Journal Article
Review

Language

eng

PubMed ID

28443673

Citation

Takano, Yoko, et al. "Fluorescent Probes and Selective Inhibitors for Biological Studies of Hydrogen Sulfide- and Polysulfide-Mediated Signaling." Antioxidants & Redox Signaling, vol. 27, no. 10, 2017, pp. 669-683.
Takano Y, Echizen H, Hanaoka K. Fluorescent Probes and Selective Inhibitors for Biological Studies of Hydrogen Sulfide- and Polysulfide-Mediated Signaling. Antioxid Redox Signal. 2017;27(10):669-683.
Takano, Y., Echizen, H., & Hanaoka, K. (2017). Fluorescent Probes and Selective Inhibitors for Biological Studies of Hydrogen Sulfide- and Polysulfide-Mediated Signaling. Antioxidants & Redox Signaling, 27(10), 669-683. https://doi.org/10.1089/ars.2017.7070
Takano Y, Echizen H, Hanaoka K. Fluorescent Probes and Selective Inhibitors for Biological Studies of Hydrogen Sulfide- and Polysulfide-Mediated Signaling. Antioxid Redox Signal. 2017 Oct 1;27(10):669-683. PubMed PMID: 28443673.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fluorescent Probes and Selective Inhibitors for Biological Studies of Hydrogen Sulfide- and Polysulfide-Mediated Signaling. AU - Takano,Yoko, AU - Echizen,Honami, AU - Hanaoka,Kenjiro, Y1 - 2017/05/18/ PY - 2017/4/27/pubmed PY - 2018/3/10/medline PY - 2017/4/27/entrez KW - fluorescence imaging KW - inhibitors KW - reactive sulfur species SP - 669 EP - 683 JF - Antioxidants & redox signaling JO - Antioxid Redox Signal VL - 27 IS - 10 N2 - SIGNIFICANCE: Hydrogen sulfide (H2S) plays roles in many physiological processes, including relaxation of vascular smooth muscles, mediation of neurotransmission, inhibition of insulin signaling, and regulation of inflammation. Also, hydropersulfide (R-S-SH) and polysulfide (-S-Sn-S-) have recently been identified as reactive sulfur species (RSS) that regulate the bioactivities of multiple proteins via S-sulfhydration of cysteine residues (protein Cys-SSH) and show cytoprotection. Chemical tools such as fluorescent probes and selective inhibitors are needed to establish in detail the physiological roles of H2S and polysulfide. Recent Advances: Although many fluorescent probes for H2S are available, fluorescent probes for hydropersulfide and polysulfide have only recently been developed and used to detect these sulfur species in living cells. CRITICAL ISSUES: In this review, we summarize recent progress in developing chemical tools for the study of H2S, hydropersulfide, and polysulfide, covering fluorescent probes based on various design strategies and selective inhibitors of H2S- and polysulfide-producing enzymes (cystathionine γ-lyase, cystathionine β-synthase, and 3-mercaptopyruvate sulfurtransferase), and we summarize their applications in biological studies. FUTURE DIRECTIONS: Despite recent progress, the precise biological functions of H2S, hydropersulfide, and polysulfide remain to be fully established. Fluorescent probes and selective inhibitors are effective chemical tools to study the physiological roles of these sulfur molecules in living cells and tissues. Therefore, further development of a broad range of practical fluorescent probes and selective inhibitors as tools for studies of RSS biology is currently attracting great interest. Antioxid. Redox Signal. 27, 669-683. SN - 1557-7716 UR - https://www.unboundmedicine.com/medline/citation/28443673/Fluorescent_Probes_and_Selective_Inhibitors_for_Biological_Studies_of_Hydrogen_Sulfide__and_Polysulfide_Mediated_Signaling_ L2 - https://www.liebertpub.com/doi/10.1089/ars.2017.7070?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -