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Model-based biotechnological potential analysis of Kluyveromyces marxianus central metabolism.
J Ind Microbiol Biotechnol. 2017 08; 44(8):1177-1190.JI

Abstract

The non-conventional yeast Kluyveromyces marxianus is an emerging industrial producer for many biotechnological processes. Here, we show the application of a biomass-linked stoichiometric model of central metabolism that is experimentally validated, and mass and charge balanced for assessing the carbon conversion efficiency of wild type and modified K. marxianus. Pairs of substrates (lactose, glucose, inulin, xylose) and products (ethanol, acetate, lactate, glycerol, ethyl acetate, succinate, glutamate, phenylethanol and phenylalanine) are examined by various modelling and optimisation methods. Our model reveals the organism's potential for industrial application and metabolic engineering. Modelling results imply that the aeration regime can be used as a tool to optimise product yield and flux distribution in K. marxianus. Also rebalancing NADH and NADPH utilisation can be used to improve the efficiency of substrate conversion. Xylose is identified as a biotechnologically promising substrate for K. marxianus.

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Authors+Show Affiliations

Pentjuss A
Institute of Microbiology and Biotechnology, University of Latvia, Jelgavas str. 1, Riga, 1004, Latvia.
Stalidzans E
Institute of Microbiology and Biotechnology, University of Latvia, Jelgavas str. 1, Riga, 1004, Latvia. stalidz@lu.lv.
Liepins J
Institute of Microbiology and Biotechnology, University of Latvia, Jelgavas str. 1, Riga, 1004, Latvia.
Kokina A
Institute of Microbiology and Biotechnology, University of Latvia, Jelgavas str. 1, Riga, 1004, Latvia.
Martynova J
Institute of Microbiology and Biotechnology, University of Latvia, Jelgavas str. 1, Riga, 1004, Latvia.
Zikmanis P
Institute of Microbiology and Biotechnology, University of Latvia, Jelgavas str. 1, Riga, 1004, Latvia.
Mozga I
Institute of Microbiology and Biotechnology, University of Latvia, Jelgavas str. 1, Riga, 1004, Latvia.
Scherbaka R
Institute of Microbiology and Biotechnology, University of Latvia, Jelgavas str. 1, Riga, 1004, Latvia.
Hartman H
Department of Biological and Medical Sciences, Oxford Brookes University, Headington, OX, OX3 0BP, UK.
Poolman MG
Department of Biological and Medical Sciences, Oxford Brookes University, Headington, OX, OX3 0BP, UK.
Fell DA
Department of Biological and Medical Sciences, Oxford Brookes University, Headington, OX, OX3 0BP, UK.
Vigants A
Institute of Microbiology and Biotechnology, University of Latvia, Jelgavas str. 1, Riga, 1004, Latvia.

MeSH

AcetatesBiomassCalibrationCulture MediaEthanolGlucoseGlutamic AcidGlycerolIndustrial MicrobiologyInulinKluyveromycesLactatesLactoseMetabolic EngineeringModels, MolecularOxygen ConsumptionPhenylalaninePhenylethyl AlcoholReproducibility of ResultsSuccinic AcidXylose

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28444480

Citation

Pentjuss, A, et al. "Model-based Biotechnological Potential Analysis of Kluyveromyces Marxianus Central Metabolism." Journal of Industrial Microbiology & Biotechnology, vol. 44, no. 8, 2017, pp. 1177-1190.
Pentjuss A, Stalidzans E, Liepins J, et al. Model-based biotechnological potential analysis of Kluyveromyces marxianus central metabolism. J Ind Microbiol Biotechnol. 2017;44(8):1177-1190.
Pentjuss, A., Stalidzans, E., Liepins, J., Kokina, A., Martynova, J., Zikmanis, P., Mozga, I., Scherbaka, R., Hartman, H., Poolman, M. G., Fell, D. A., & Vigants, A. (2017). Model-based biotechnological potential analysis of Kluyveromyces marxianus central metabolism. Journal of Industrial Microbiology & Biotechnology, 44(8), 1177-1190. https://doi.org/10.1007/s10295-017-1946-8
Pentjuss A, et al. Model-based Biotechnological Potential Analysis of Kluyveromyces Marxianus Central Metabolism. J Ind Microbiol Biotechnol. 2017;44(8):1177-1190. PubMed PMID: 28444480.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Model-based biotechnological potential analysis of Kluyveromyces marxianus central metabolism. AU - Pentjuss,A, AU - Stalidzans,E, AU - Liepins,J, AU - Kokina,A, AU - Martynova,J, AU - Zikmanis,P, AU - Mozga,I, AU - Scherbaka,R, AU - Hartman,H, AU - Poolman,M G, AU - Fell,D A, AU - Vigants,A, Y1 - 2017/04/25/ PY - 2016/10/20/received PY - 2017/04/16/accepted PY - 2017/4/27/pubmed PY - 2018/1/2/medline PY - 2017/4/27/entrez KW - Central metabolism KW - Essentiality analysis KW - Kluyveromyces marxianus KW - Metabolic engineering KW - Modelling SP - 1177 EP - 1190 JF - Journal of industrial microbiology & biotechnology JO - J Ind Microbiol Biotechnol VL - 44 IS - 8 N2 - The non-conventional yeast Kluyveromyces marxianus is an emerging industrial producer for many biotechnological processes. Here, we show the application of a biomass-linked stoichiometric model of central metabolism that is experimentally validated, and mass and charge balanced for assessing the carbon conversion efficiency of wild type and modified K. marxianus. Pairs of substrates (lactose, glucose, inulin, xylose) and products (ethanol, acetate, lactate, glycerol, ethyl acetate, succinate, glutamate, phenylethanol and phenylalanine) are examined by various modelling and optimisation methods. Our model reveals the organism's potential for industrial application and metabolic engineering. Modelling results imply that the aeration regime can be used as a tool to optimise product yield and flux distribution in K. marxianus. Also rebalancing NADH and NADPH utilisation can be used to improve the efficiency of substrate conversion. Xylose is identified as a biotechnologically promising substrate for K. marxianus. SN - 1476-5535 UR - https://www.unboundmedicine.com/medline/citation/28444480/Model_based_biotechnological_potential_analysis_of_Kluyveromyces_marxianus_central_metabolism_ L2 - http://dx.doi.org/10.1007/s10295-017-1946-8 DB - PRIME DP - Unbound Medicine ER -