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Use of molecular markers in identification and characterization of resistance to rice blast in India.
PLoS One 2017; 12(4):e0176236Plos

Abstract

Rice blast disease caused by Magnaporthe oryzae is one of the most destructive disease causing huge losses to rice yield in different parts of the world. Therefore, an attempt has been made to find out the resistance by screening and studying the genetic diversity of eighty released rice varieties by National Rice Research Institute, Cuttack (NRVs) using molecular markers linked to twelve major blast resistance (R) genes viz Pib, Piz, Piz-t, Pik, Pik-p, Pikm Pik-h, Pita/Pita-2, Pi2, Pi9, Pi1 and Pi5. Out of which, nineteen varieties (23.75%) showed resistance, twenty one were moderately resistant (26.25%) while remaining forty varieties (50%) showed susceptible in uniform blast nursery. Rice varieties possessing blast resistance genes varied from four to twelve and the frequencies of the resistance genes ranged from 0 to 100%. The cluster analysis grouped the eighty NRVs into two major clusters at 63% level of genetic similarity coefficient. The PIC value for seventeen markers varied from 0 to 0.37 at an average of 0.20. Out of seventeen markers, only five markers, 195R-1, Pi9-i, Pita3, YL155/YL87 and 40N23r corresponded to three broad spectrum R genes viz. Pi9, Pita/Pita2 and Pi5 were found to be significantly associated with the blast disease with explaining phenotypic variance from 3.5% to 7.7%. The population structure analysis and PCoA divided the entire 80 NRVs into two sub-groups. The outcome of this study would help to formulate strategies for improving rice blast resistance through genetic studies, plant-pathogen interaction, identification of novel R genes, development of new resistant varieties through marker-assisted breeding for improving rice blast resistance in India and worldwide.

Authors+Show Affiliations

ICAR-National Rice Research Institute, Cuttack, Odisha, India.ICAR-National Rice Research Institute, Cuttack, Odisha, India.ICAR-National Rice Research Institute, Cuttack, Odisha, India.ICAR-National Rice Research Institute, Cuttack, Odisha, India.ICAR-National Rice Research Institute, Cuttack, Odisha, India.ICAR-National Rice Research Institute, Cuttack, Odisha, India.ICAR-National Rice Research Institute, Cuttack, Odisha, India.ICAR-National Rice Research Institute, Cuttack, Odisha, India.ICAR-National Rice Research Institute, Cuttack, Odisha, India.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28445532

Citation

Yadav, Manoj Kumar, et al. "Use of Molecular Markers in Identification and Characterization of Resistance to Rice Blast in India." PloS One, vol. 12, no. 4, 2017, pp. e0176236.
Yadav MK, S A, Ngangkham U, et al. Use of molecular markers in identification and characterization of resistance to rice blast in India. PLoS ONE. 2017;12(4):e0176236.
Yadav, M. K., S, A., Ngangkham, U., Shubudhi, H. N., Bag, M. K., Adak, T., ... Jena, M. (2017). Use of molecular markers in identification and characterization of resistance to rice blast in India. PloS One, 12(4), pp. e0176236. doi:10.1371/journal.pone.0176236.
Yadav MK, et al. Use of Molecular Markers in Identification and Characterization of Resistance to Rice Blast in India. PLoS ONE. 2017;12(4):e0176236. PubMed PMID: 28445532.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Use of molecular markers in identification and characterization of resistance to rice blast in India. AU - Yadav,Manoj Kumar, AU - S,Aravindan, AU - Ngangkham,Umakanta, AU - Shubudhi,H N, AU - Bag,Manas Kumar, AU - Adak,Totan, AU - Munda,Sushmita, AU - Samantaray,Sanghamitra, AU - Jena,Mayabini, Y1 - 2017/04/26/ PY - 2016/09/28/received PY - 2017/04/08/accepted PY - 2017/4/27/entrez PY - 2017/4/27/pubmed PY - 2017/9/7/medline SP - e0176236 EP - e0176236 JF - PloS one JO - PLoS ONE VL - 12 IS - 4 N2 - Rice blast disease caused by Magnaporthe oryzae is one of the most destructive disease causing huge losses to rice yield in different parts of the world. Therefore, an attempt has been made to find out the resistance by screening and studying the genetic diversity of eighty released rice varieties by National Rice Research Institute, Cuttack (NRVs) using molecular markers linked to twelve major blast resistance (R) genes viz Pib, Piz, Piz-t, Pik, Pik-p, Pikm Pik-h, Pita/Pita-2, Pi2, Pi9, Pi1 and Pi5. Out of which, nineteen varieties (23.75%) showed resistance, twenty one were moderately resistant (26.25%) while remaining forty varieties (50%) showed susceptible in uniform blast nursery. Rice varieties possessing blast resistance genes varied from four to twelve and the frequencies of the resistance genes ranged from 0 to 100%. The cluster analysis grouped the eighty NRVs into two major clusters at 63% level of genetic similarity coefficient. The PIC value for seventeen markers varied from 0 to 0.37 at an average of 0.20. Out of seventeen markers, only five markers, 195R-1, Pi9-i, Pita3, YL155/YL87 and 40N23r corresponded to three broad spectrum R genes viz. Pi9, Pita/Pita2 and Pi5 were found to be significantly associated with the blast disease with explaining phenotypic variance from 3.5% to 7.7%. The population structure analysis and PCoA divided the entire 80 NRVs into two sub-groups. The outcome of this study would help to formulate strategies for improving rice blast resistance through genetic studies, plant-pathogen interaction, identification of novel R genes, development of new resistant varieties through marker-assisted breeding for improving rice blast resistance in India and worldwide. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/28445532/Use_of_molecular_markers_in_identification_and_characterization_of_resistance_to_rice_blast_in_India_ L2 - http://dx.plos.org/10.1371/journal.pone.0176236 DB - PRIME DP - Unbound Medicine ER -