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Natural products isolated from Tetragonula carbonaria cerumen modulate free radical-scavenging and 5-lipoxygenase activities in vitro.
BMC Complement Altern Med. 2017 Apr 26; 17(1):232.BC

Abstract

BACKGROUND

Propolis and cerumen are plant-derived products found in honeybees and stingless bees, respectively. Although propolis is an ancient folk medicine, the bioactivities of cerumen obtained from Australian native stingless bees (Tetragonula carbonaria) have not been widely studied. Therefore, we investigated selected anti-oxidant and anti-inflammatory properties of T. carbonaria cerumen.

METHODS

A methanolic extract was prepared from the combined cerumen of 40 T. carbonaria hives, and HPLC was used to screen for chemical constituents that scavenged 2,2-azobis(2-methylpropionamidine) dihydrochloride (AAPH). The ability of cerumen extracts to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH) and to interfere with leukotriene B4 (LTB4) production in ionomycin-stimulated human neutrophils was also examined.

RESULTS

The extract dose-dependently scavenged DPPH (EC50 = 27.0 ± 2.3 μg/mL); and inhibited the 5-lipoxygenase (5-LOX)-mediated oxidation of linoleic acid (IC50 = 67.1 ± 9.6 μg/mL). Pre-treatment of isolated human neutrophils with the methanolic cerumen extract additionally inhibited the ionomycin-stimulated production of LTB4 from these cells (IC50 = 13.3 ± 5.3 μg/mL). Following multi-solvent extraction, the free radical-scavenging and 5-LOX-inhibiting activities of the initial cerumen extract were retained in a polar, methanol-water extract, which contained gallic acid and a range of flavonone and phenolic natural products.

CONCLUSIONS

The findings identify free radical scavenging activity, and interference by extracts of T. carbonaria cerumen in 5-LOX-LTB4 signaling. Further investigation is needed to determine whether the extracts will provide therapeutic benefits for medical conditions in which oxidative stress and inflammation are implicated, including cardiovascular disease and impaired wound healing.

Authors+Show Affiliations

Inflammation and Healing Research Cluster, Faculty of Science, Health, Education and Engineering, School of Health and Sport Sciences, University of the Sunshine Coast, Maroochydore, QLD, 4558, Australia.Inflammation and Healing Research Cluster, Faculty of Science, Health, Education and Engineering, School of Health and Sport Sciences, University of the Sunshine Coast, Maroochydore, QLD, 4558, Australia. GeneCology Research Centre, Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, Maroochydore, QLD, Australia.GeneCology Research Centre, Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, Maroochydore, QLD, Australia.Inflammation and Healing Research Cluster, Faculty of Science, Health, Education and Engineering, School of Health and Sport Sciences, University of the Sunshine Coast, Maroochydore, QLD, 4558, Australia. FRussell@usc.edu.au.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28446157

Citation

Hamilton, Karina D., et al. "Natural Products Isolated From Tetragonula Carbonaria Cerumen Modulate Free Radical-scavenging and 5-lipoxygenase Activities in Vitro." BMC Complementary and Alternative Medicine, vol. 17, no. 1, 2017, p. 232.
Hamilton KD, Brooks PR, Ogbourne SM, et al. Natural products isolated from Tetragonula carbonaria cerumen modulate free radical-scavenging and 5-lipoxygenase activities in vitro. BMC Complement Altern Med. 2017;17(1):232.
Hamilton, K. D., Brooks, P. R., Ogbourne, S. M., & Russell, F. D. (2017). Natural products isolated from Tetragonula carbonaria cerumen modulate free radical-scavenging and 5-lipoxygenase activities in vitro. BMC Complementary and Alternative Medicine, 17(1), 232. https://doi.org/10.1186/s12906-017-1748-6
Hamilton KD, et al. Natural Products Isolated From Tetragonula Carbonaria Cerumen Modulate Free Radical-scavenging and 5-lipoxygenase Activities in Vitro. BMC Complement Altern Med. 2017 Apr 26;17(1):232. PubMed PMID: 28446157.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Natural products isolated from Tetragonula carbonaria cerumen modulate free radical-scavenging and 5-lipoxygenase activities in vitro. AU - Hamilton,Karina D, AU - Brooks,Peter R, AU - Ogbourne,Steven M, AU - Russell,Fraser D, Y1 - 2017/04/26/ PY - 2016/12/21/received PY - 2017/04/21/accepted PY - 2017/4/28/entrez PY - 2017/4/28/pubmed PY - 2017/8/2/medline KW - 5-lipoxygenase KW - Free radical-scavenging KW - Inflammation KW - Propolis KW - Tetragonula carbonaria SP - 232 EP - 232 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 17 IS - 1 N2 - BACKGROUND: Propolis and cerumen are plant-derived products found in honeybees and stingless bees, respectively. Although propolis is an ancient folk medicine, the bioactivities of cerumen obtained from Australian native stingless bees (Tetragonula carbonaria) have not been widely studied. Therefore, we investigated selected anti-oxidant and anti-inflammatory properties of T. carbonaria cerumen. METHODS: A methanolic extract was prepared from the combined cerumen of 40 T. carbonaria hives, and HPLC was used to screen for chemical constituents that scavenged 2,2-azobis(2-methylpropionamidine) dihydrochloride (AAPH). The ability of cerumen extracts to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH) and to interfere with leukotriene B4 (LTB4) production in ionomycin-stimulated human neutrophils was also examined. RESULTS: The extract dose-dependently scavenged DPPH (EC50 = 27.0 ± 2.3 μg/mL); and inhibited the 5-lipoxygenase (5-LOX)-mediated oxidation of linoleic acid (IC50 = 67.1 ± 9.6 μg/mL). Pre-treatment of isolated human neutrophils with the methanolic cerumen extract additionally inhibited the ionomycin-stimulated production of LTB4 from these cells (IC50 = 13.3 ± 5.3 μg/mL). Following multi-solvent extraction, the free radical-scavenging and 5-LOX-inhibiting activities of the initial cerumen extract were retained in a polar, methanol-water extract, which contained gallic acid and a range of flavonone and phenolic natural products. CONCLUSIONS: The findings identify free radical scavenging activity, and interference by extracts of T. carbonaria cerumen in 5-LOX-LTB4 signaling. Further investigation is needed to determine whether the extracts will provide therapeutic benefits for medical conditions in which oxidative stress and inflammation are implicated, including cardiovascular disease and impaired wound healing. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/28446157/Natural_products_isolated_from_Tetragonula_carbonaria_cerumen_modulate_free_radical_scavenging_and_5_lipoxygenase_activities_in_vitro_ L2 - https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/s12906-017-1748-6 DB - PRIME DP - Unbound Medicine ER -