Tags

Type your tag names separated by a space and hit enter

[Cloning and localization of the replication and mobilization regions in the D-plasmid of Pseudomonas putida pBS286 (IncP-9) with a broad host range].
Genetika. 1988 Jun; 24(6):980-92.G

Abstract

Rep-mob loci of naphthalene degradative plasmid pBS286 (IncP-9) have been cloned on the Escherichia coli vectors pUC19 and pUBR322. These loci confer to recombinant plasmids pBS952 and pBS953 the ability for effective mobilization by RP4 (IncP-1) and F plasmid, as well as constant maintenance in various gram-negative bacteria. Localization of cloned sequences in the restriction fragments of conservative part of the pBS286 genome was established. The data obtained correlate with the analysis of plasmids pBS950 and pBS951 which are spontaneous mini-derivatives of pBS286 and pBS292 (delta NPL1::Tn1/Tra+ Nah-) plasmids formed during transformation of E. coli HB101 cells. Plasmids pBS952 and pBS953 retain the incompatibility properties of parental IncP-9 replicon. These recombinant derivatives can be used for construction of bhr vectors with required properties and compatible with bhr vectors constructed on the basis of plasmids from the IncP-1 and IncP-4 groups.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

rus

PubMed ID

2844624

Citation

Tsoĭ, T V., et al. "[Cloning and Localization of the Replication and Mobilization Regions in the D-plasmid of Pseudomonas Putida pBS286 (IncP-9) With a Broad Host Range]." Genetika, vol. 24, no. 6, 1988, pp. 980-92.
Tsoĭ TV, Gribanova LK, Kosheleva IA, et al. [Cloning and localization of the replication and mobilization regions in the D-plasmid of Pseudomonas putida pBS286 (IncP-9) with a broad host range]. Genetika. 1988;24(6):980-92.
Tsoĭ, T. V., Gribanova, L. K., Kosheleva, I. A., & Boronin, A. M. (1988). [Cloning and localization of the replication and mobilization regions in the D-plasmid of Pseudomonas putida pBS286 (IncP-9) with a broad host range]. Genetika, 24(6), 980-92.
Tsoĭ TV, et al. [Cloning and Localization of the Replication and Mobilization Regions in the D-plasmid of Pseudomonas Putida pBS286 (IncP-9) With a Broad Host Range]. Genetika. 1988;24(6):980-92. PubMed PMID: 2844624.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Cloning and localization of the replication and mobilization regions in the D-plasmid of Pseudomonas putida pBS286 (IncP-9) with a broad host range]. AU - Tsoĭ,T V, AU - Gribanova,L K, AU - Kosheleva,I A, AU - Boronin,A M, PY - 1988/6/1/pubmed PY - 1988/6/1/medline PY - 1988/6/1/entrez SP - 980 EP - 92 JF - Genetika JO - Genetika VL - 24 IS - 6 N2 - Rep-mob loci of naphthalene degradative plasmid pBS286 (IncP-9) have been cloned on the Escherichia coli vectors pUC19 and pUBR322. These loci confer to recombinant plasmids pBS952 and pBS953 the ability for effective mobilization by RP4 (IncP-1) and F plasmid, as well as constant maintenance in various gram-negative bacteria. Localization of cloned sequences in the restriction fragments of conservative part of the pBS286 genome was established. The data obtained correlate with the analysis of plasmids pBS950 and pBS951 which are spontaneous mini-derivatives of pBS286 and pBS292 (delta NPL1::Tn1/Tra+ Nah-) plasmids formed during transformation of E. coli HB101 cells. Plasmids pBS952 and pBS953 retain the incompatibility properties of parental IncP-9 replicon. These recombinant derivatives can be used for construction of bhr vectors with required properties and compatible with bhr vectors constructed on the basis of plasmids from the IncP-1 and IncP-4 groups. SN - 0016-6758 UR - https://www.unboundmedicine.com/medline/citation/2844624/[Cloning_and_localization_of_the_replication_and_mobilization_regions_in_the_D_plasmid_of_Pseudomonas_putida_pBS286__IncP_9__with_a_broad_host_range]_ DB - PRIME DP - Unbound Medicine ER -