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The evaluation of diagnostic efficiency for stromal herpes simplex keratitis by the combination of tear HSV-sIgA and HSV-DNA.
Graefes Arch Clin Exp Ophthalmol 2017; 255(7):1409-1415GA

Abstract

PURPOSE

To assess the differential diagnostic values for stromal herpes simplex keratitis (HSK) by using tear HSV-sIgA, tear HSV-DNA, and the combination.

METHODS

Tear samples for both eyes and the paired serum were collected from 187 stromal HSK and 56 controls. Enzyme-linked immune sorbent assay (ELISA) was used to analyze the tear HSV-sIgA and serum IgG/IgM/IgA. The levels of tear HSV-DNA were measured by polymerase chain reaction (PCR).

RESULTS

The positive rates for tear HSV-sIgA and HSV-DNA were 36.90% and 10.96% respectively in stromal HSK patients. Twelve showed positivity for both sIgA and DNA, while 46 cases were positive for sIgA or DNA. The sensitivity, specificity, PPV, and NPV for simultaneous measurement were 39.73%, 98.21%, 98.31%, and 38.46%. The total negative conversion rate of sIgA was 95.71%.

CONCLUSIONS

The diagnostic efficiency of HSV-sIgA only is nearly equal to the combination of HSV-sIgA and HSV-DNA, and the positive result is optimum to achieve a reliable diagnosis of stromal HSK even in atypical or unsuspected cases.

Authors+Show Affiliations

Department of Ophthalmology, Eye and ENT Hospital of Fudan University, Shanghai, China. The Key Lab of Myopia, Ministry of Health, Shanghai, People's Republic of China.Department of Ophthalmology, Eye and ENT Hospital of Fudan University, Shanghai, China. The Key Lab of Myopia, Ministry of Health, Shanghai, People's Republic of China.Department of Ophthalmology, Eye and ENT Hospital of Fudan University, Shanghai, China.Department of Ophthalmology, Eye and ENT Hospital of Fudan University, Shanghai, China. The Key Lab of Myopia, Ministry of Health, Shanghai, People's Republic of China.Department of Ophthalmology, Eye and ENT Hospital of Fudan University, Shanghai, China. fdeent@126.com. The Key Lab of Myopia, Ministry of Health, Shanghai, People's Republic of China. fdeent@126.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28451758

Citation

Qiu, Jini, et al. "The Evaluation of Diagnostic Efficiency for Stromal Herpes Simplex Keratitis By the Combination of Tear HSV-sIgA and HSV-DNA." Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Fur Klinische Und Experimentelle Ophthalmologie, vol. 255, no. 7, 2017, pp. 1409-1415.
Qiu J, Huang F, Wang Z, et al. The evaluation of diagnostic efficiency for stromal herpes simplex keratitis by the combination of tear HSV-sIgA and HSV-DNA. Graefes Arch Clin Exp Ophthalmol. 2017;255(7):1409-1415.
Qiu, J., Huang, F., Wang, Z., Xu, J., & Zhang, C. (2017). The evaluation of diagnostic efficiency for stromal herpes simplex keratitis by the combination of tear HSV-sIgA and HSV-DNA. Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Fur Klinische Und Experimentelle Ophthalmologie, 255(7), pp. 1409-1415. doi:10.1007/s00417-017-3653-6.
Qiu J, et al. The Evaluation of Diagnostic Efficiency for Stromal Herpes Simplex Keratitis By the Combination of Tear HSV-sIgA and HSV-DNA. Graefes Arch Clin Exp Ophthalmol. 2017;255(7):1409-1415. PubMed PMID: 28451758.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The evaluation of diagnostic efficiency for stromal herpes simplex keratitis by the combination of tear HSV-sIgA and HSV-DNA. AU - Qiu,Jini, AU - Huang,Feifei, AU - Wang,Zhujian, AU - Xu,Jianjiang, AU - Zhang,Chaoran, Y1 - 2017/04/27/ PY - 2016/12/22/received PY - 2017/03/20/accepted PY - 2017/03/12/revised PY - 2017/4/30/pubmed PY - 2017/11/29/medline PY - 2017/4/29/entrez KW - Diagnosis KW - Stromal HSK KW - Tear HSV-DNA KW - Tear HSV-sIgA SP - 1409 EP - 1415 JF - Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie JO - Graefes Arch. Clin. Exp. Ophthalmol. VL - 255 IS - 7 N2 - PURPOSE: To assess the differential diagnostic values for stromal herpes simplex keratitis (HSK) by using tear HSV-sIgA, tear HSV-DNA, and the combination. METHODS: Tear samples for both eyes and the paired serum were collected from 187 stromal HSK and 56 controls. Enzyme-linked immune sorbent assay (ELISA) was used to analyze the tear HSV-sIgA and serum IgG/IgM/IgA. The levels of tear HSV-DNA were measured by polymerase chain reaction (PCR). RESULTS: The positive rates for tear HSV-sIgA and HSV-DNA were 36.90% and 10.96% respectively in stromal HSK patients. Twelve showed positivity for both sIgA and DNA, while 46 cases were positive for sIgA or DNA. The sensitivity, specificity, PPV, and NPV for simultaneous measurement were 39.73%, 98.21%, 98.31%, and 38.46%. The total negative conversion rate of sIgA was 95.71%. CONCLUSIONS: The diagnostic efficiency of HSV-sIgA only is nearly equal to the combination of HSV-sIgA and HSV-DNA, and the positive result is optimum to achieve a reliable diagnosis of stromal HSK even in atypical or unsuspected cases. SN - 1435-702X UR - https://www.unboundmedicine.com/medline/citation/28451758/The_evaluation_of_diagnostic_efficiency_for_stromal_herpes_simplex_keratitis_by_the_combination_of_tear_HSV_sIgA_and_HSV_DNA_ L2 - https://dx.doi.org/10.1007/s00417-017-3653-6 DB - PRIME DP - Unbound Medicine ER -