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Palmitoleic Acid Inhibits RANKL-Induced Osteoclastogenesis and Bone Resorption by Suppressing NF-κB and MAPK Signalling Pathways.
Nutrients. 2017 Apr 28; 9(5)N

Abstract

Osteoclasts are large, multinucleated cells that are responsible for the breakdown or resorption of bone during bone remodelling. Studies have shown that certain fatty acids (FAs) can increase bone formation, reduce bone loss, and influence total bone mass. Palmitoleic acid (PLA) is a 16-carbon, monounsaturated FA that has shown anti-inflammatory properties similar to other FAs. The effects of PLA in bone remain unexplored. Here we investigated the effects of PLA on receptor activator of nuclear factor kappa B (NF-κB) ligand (RANKL)-induced osteoclast formation and bone resorption in RAW264.7 murine macrophages. PLA decreased the number of large, multinucleated tartrate resistant acid phosphatase (TRAP) positive osteoclasts and furthermore, suppressed the osteolytic capability of these osteoclasts. This was accompanied by a decrease in expression of resorption markers (Trap, matrix metalloproteinase 9 (Mmp9), cathepsin K (Ctsk)). PLA further decreased the expression of genes involved in the formation and function of osteoclasts. Additionally, PLA inhibited NF-κB activity and the activation of mitogen activated protein kinases (MAPK), c-Jun N-terminal kinase (JNK) and extracellular signal-regulated kinase (ERK). Moreover, PLA induced apoptosis in mature osteoclasts. This study reveals that PLA inhibits RANKL-induced osteoclast formation in RAW264.7 murine macrophages through suppression of NF-κB and MAPK signalling pathways. This may indicate that PLA has potential as a therapeutic for bone diseases characterized by excessive osteoclast formation.

Authors+Show Affiliations

Department of Physiology, University of Pretoria, Pretoria 0001, South Africa. bernadettevanheerden@ymail.com.Department of Physiology, University of Pretoria, Pretoria 0001, South Africa. abe.kasonga@up.ac.za.School of Food and Nutrition, Massey Institute of Food Science and Technology, Massey University, Palmerston North 4442, New Zealand. m.c.kruger@massey.ac.nz. Institute for Food, Nutrition and Well-being, University of Pretoria, Pretoria 0001, South Africa. m.c.kruger@massey.ac.nz.Department of Physiology, University of Pretoria, Pretoria 0001, South Africa. magdalena.coetzee@up.ac.za. Institute for Food, Nutrition and Well-being, University of Pretoria, Pretoria 0001, South Africa. magdalena.coetzee@up.ac.za.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28452958

Citation

van Heerden, Bernadette, et al. "Palmitoleic Acid Inhibits RANKL-Induced Osteoclastogenesis and Bone Resorption By Suppressing NF-κB and MAPK Signalling Pathways." Nutrients, vol. 9, no. 5, 2017.
van Heerden B, Kasonga A, Kruger MC, et al. Palmitoleic Acid Inhibits RANKL-Induced Osteoclastogenesis and Bone Resorption by Suppressing NF-κB and MAPK Signalling Pathways. Nutrients. 2017;9(5).
van Heerden, B., Kasonga, A., Kruger, M. C., & Coetzee, M. (2017). Palmitoleic Acid Inhibits RANKL-Induced Osteoclastogenesis and Bone Resorption by Suppressing NF-κB and MAPK Signalling Pathways. Nutrients, 9(5). https://doi.org/10.3390/nu9050441
van Heerden B, et al. Palmitoleic Acid Inhibits RANKL-Induced Osteoclastogenesis and Bone Resorption By Suppressing NF-κB and MAPK Signalling Pathways. Nutrients. 2017 Apr 28;9(5) PubMed PMID: 28452958.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Palmitoleic Acid Inhibits RANKL-Induced Osteoclastogenesis and Bone Resorption by Suppressing NF-κB and MAPK Signalling Pathways. AU - van Heerden,Bernadette, AU - Kasonga,Abe, AU - Kruger,Marlena C, AU - Coetzee,Magdalena, Y1 - 2017/04/28/ PY - 2017/02/07/received PY - 2017/04/18/revised PY - 2017/04/19/accepted PY - 2017/4/29/entrez PY - 2017/4/30/pubmed PY - 2018/4/13/medline KW - bone resorption KW - fatty acid KW - osteoclasts KW - palmitoleic acid JF - Nutrients JO - Nutrients VL - 9 IS - 5 N2 - Osteoclasts are large, multinucleated cells that are responsible for the breakdown or resorption of bone during bone remodelling. Studies have shown that certain fatty acids (FAs) can increase bone formation, reduce bone loss, and influence total bone mass. Palmitoleic acid (PLA) is a 16-carbon, monounsaturated FA that has shown anti-inflammatory properties similar to other FAs. The effects of PLA in bone remain unexplored. Here we investigated the effects of PLA on receptor activator of nuclear factor kappa B (NF-κB) ligand (RANKL)-induced osteoclast formation and bone resorption in RAW264.7 murine macrophages. PLA decreased the number of large, multinucleated tartrate resistant acid phosphatase (TRAP) positive osteoclasts and furthermore, suppressed the osteolytic capability of these osteoclasts. This was accompanied by a decrease in expression of resorption markers (Trap, matrix metalloproteinase 9 (Mmp9), cathepsin K (Ctsk)). PLA further decreased the expression of genes involved in the formation and function of osteoclasts. Additionally, PLA inhibited NF-κB activity and the activation of mitogen activated protein kinases (MAPK), c-Jun N-terminal kinase (JNK) and extracellular signal-regulated kinase (ERK). Moreover, PLA induced apoptosis in mature osteoclasts. This study reveals that PLA inhibits RANKL-induced osteoclast formation in RAW264.7 murine macrophages through suppression of NF-κB and MAPK signalling pathways. This may indicate that PLA has potential as a therapeutic for bone diseases characterized by excessive osteoclast formation. SN - 2072-6643 UR - https://www.unboundmedicine.com/medline/citation/28452958/Palmitoleic_Acid_Inhibits_RANKL_Induced_Osteoclastogenesis_and_Bone_Resorption_by_Suppressing_NF_κB_and_MAPK_Signalling_Pathways_ L2 - https://www.mdpi.com/resolver?pii=nu9050441 DB - PRIME DP - Unbound Medicine ER -