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A comparison of rat degloving injury models.
Acta Orthop Traumatol Turc. 2017 Jul; 51(4):308-312.AO

Abstract

OBJECTIVE

Two different rat models for degloving injury were described in the literature. Our aim in this study is to compare these rat models to determine which one is more reliable and reproducible.

METHODS

We surgically induced degloving injury on tails and left hindlimbs of Wistar albino rats (n = 8), and sutured the avulsed tissues back in their original positions after a waiting period. We observed the changes in the avulsed flaps every other day for 10 days. At the end of follow-up period we evaluated the lesions in avulsed flaps by macroscopic measurement of necrosis and histological ulcer scoring using the National Pressure Ulcer Advisory Panel (NPUAP) Scale.

RESULTS

The average length of necrosis in avulsed tail flaps was 28.42 ± 3.04 mm, whereas there was no necrosis in avulsed hindlimb flaps (p < 0.05). The average ulcer score of the lesions in tail and left hindlimb were 3.42 ± 0.78, and 1.28 ± 0.48, respectively (p < 0.05). Despite the lack of visible necrosis TUNEL staining revealed an increased amount of apoptotic cells in avulsed hindlimb flaps. Literature review revealed a significant variability in previous studies in terms of the amount of necrosis observed in tail degloving injury model.

CONCLUSION

Tail degloving injury model proved to be a more reliable animal model for degloving injuries. However, standardization of the magnitude of degloving force is required to decrease the variability of necrosis observed in the literature.

Authors+Show Affiliations

Fırat Universitesi Tıp Fakultesi, Department of Plastic Surgery, Elazig, Turkey. Electronic address: saltun@firat.edu.tr.University of California - Davis Medical Center, Department of Surgery, Division of Plastic Surgery, Sacramento, CA, USA.Fırat Universitesi Tıp Fakultesi, Department of Plastic Surgery, Elazig, Turkey.Fırat Universitesi Tıp Fakultesi, Department of Plastic Surgery, Elazig, Turkey.Fırat Universitesi Tıp Fakultesi, Department of Plastic Surgery, Elazig, Turkey.Başkent Universitesi, Konya Uygulama ve Araştırma Hastanesi, Department of Plastic Surgery, Konya, Turkey.Fırat Universitesi Tıp Fakultesi, Department of Plastic Surgery, Elazig, Turkey.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28454780

Citation

Altun, Serdar, et al. "A Comparison of Rat Degloving Injury Models." Acta Orthopaedica Et Traumatologica Turcica, vol. 51, no. 4, 2017, pp. 308-312.
Altun S, Orbay H, Ekinci M, et al. A comparison of rat degloving injury models. Acta Orthop Traumatol Turc. 2017;51(4):308-312.
Altun, S., Orbay, H., Ekinci, M., Cetinbas, A., Bal, A., Arpaci, E., & Okur, M. İ. (2017). A comparison of rat degloving injury models. Acta Orthopaedica Et Traumatologica Turcica, 51(4), 308-312. https://doi.org/10.1016/j.aott.2017.03.007
Altun S, et al. A Comparison of Rat Degloving Injury Models. Acta Orthop Traumatol Turc. 2017;51(4):308-312. PubMed PMID: 28454780.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A comparison of rat degloving injury models. AU - Altun,Serdar, AU - Orbay,Hakan, AU - Ekinci,Mehmet, AU - Cetinbas,Ahmet, AU - Bal,Ali, AU - Arpaci,Enver, AU - Okur,Mehmet İhsan, Y1 - 2017/04/25/ PY - 2016/07/12/received PY - 2016/09/26/revised PY - 2017/03/19/accepted PY - 2017/4/30/pubmed PY - 2018/4/24/medline PY - 2017/4/30/entrez KW - Degloving injury KW - Hindlimb KW - Rat KW - Tail SP - 308 EP - 312 JF - Acta orthopaedica et traumatologica turcica JO - Acta Orthop Traumatol Turc VL - 51 IS - 4 N2 - OBJECTIVE: Two different rat models for degloving injury were described in the literature. Our aim in this study is to compare these rat models to determine which one is more reliable and reproducible. METHODS: We surgically induced degloving injury on tails and left hindlimbs of Wistar albino rats (n = 8), and sutured the avulsed tissues back in their original positions after a waiting period. We observed the changes in the avulsed flaps every other day for 10 days. At the end of follow-up period we evaluated the lesions in avulsed flaps by macroscopic measurement of necrosis and histological ulcer scoring using the National Pressure Ulcer Advisory Panel (NPUAP) Scale. RESULTS: The average length of necrosis in avulsed tail flaps was 28.42 ± 3.04 mm, whereas there was no necrosis in avulsed hindlimb flaps (p < 0.05). The average ulcer score of the lesions in tail and left hindlimb were 3.42 ± 0.78, and 1.28 ± 0.48, respectively (p < 0.05). Despite the lack of visible necrosis TUNEL staining revealed an increased amount of apoptotic cells in avulsed hindlimb flaps. Literature review revealed a significant variability in previous studies in terms of the amount of necrosis observed in tail degloving injury model. CONCLUSION: Tail degloving injury model proved to be a more reliable animal model for degloving injuries. However, standardization of the magnitude of degloving force is required to decrease the variability of necrosis observed in the literature. SN - 2589-1294 UR - https://www.unboundmedicine.com/medline/citation/28454780/A_comparison_of_rat_degloving_injury_models_ L2 - https://doi.org/10.1016/j.aott.2017.03.007 DB - PRIME DP - Unbound Medicine ER -