Tags

Type your tag names separated by a space and hit enter

Dynamic nucleolar activity in wheat × Aegilops hybrids: evidence of C-genome dominance.
Plant Cell Rep. 2017 Aug; 36(8):1277-1285.PC

Abstract

KEY MESSAGE

NOR loci of C-subgenome are dominant in wheat × Aegilops interspecific hybrids, which may have evolutionary implications for wheat group genome dynamics and evolution. After interspecific hybridisation, some genes are often expressed from only one of the progenitor species, shaping subsequent allopolyploid genome evolution processes. A well-known example is nucleolar dominance, i.e. the formation of cell nucleoli from chromosomes of only one parental species. We studied nucleolar organizing regions (NORs) in diploid Aegilops markgrafii (syn: Ae. caudata; CC), Ae. umbellulata (UU), allotetraploids Aegilops cylindrica (CcCcDcDc) and Ae. triuncialis (CtCtUtUt), synthetic interspecific F1 hybrids between these two allotetraploids and bread wheat (Triticum aestivum, AABBDD) and in F3 generation hybrids with genome composition AABBDDCtCtUtUt using silver staining and fluorescence in situ hybridization (FISH). In Ae. markgrafii (CC), NORs of both 1C and 5C or only 5C chromosome pairs were active in different individual cells, while only NORs on 1U chromosomes were active in Ae. umbellulata (UU). Although all 35S rDNA loci of the Ct subgenome (located on 1Ct and 5Ct) were active in Ae. triuncialis, only one pair (occupying either 1Cc or 5Cc) was active in Ae. cylindrica, depending on the genotype studied. These C-genome expression patterns were transmitted to the F1 and F3 generations. Wheat chromosome NOR activity was variable in Ae. triuncialis × T. aestivum F1 seeds, but silenced by the F3 generation. No effect of maternal or paternal cross direction was observed. These results indicate that C-subgenome NOR loci are dominant in wheat × Aegilops interspecific hybrids, which may have evolutionary implications for wheat group genome dynamics and allopolyploid evolution.

Authors+Show Affiliations

Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Kurdistan, P. O. Box 416, Sanandaj, Iran. gh.mirzaghaderi@uok.ac.ir.Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Kurdistan, P. O. Box 416, Sanandaj, Iran.Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Kurdistan, P. O. Box 416, Sanandaj, Iran.Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Kurdistan, P. O. Box 416, Sanandaj, Iran.Department of Plant Breeding, IFZ Research Centre for Biosystems, Land Use and Nutrition, Justus Liebig University, Heinrich-Buff-Ring 26-32, 35392, Giessen, Germany.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28456843

Citation

Mirzaghaderi, Ghader, et al. "Dynamic Nucleolar Activity in Wheat × Aegilops Hybrids: Evidence of C-genome Dominance." Plant Cell Reports, vol. 36, no. 8, 2017, pp. 1277-1285.
Mirzaghaderi G, Abdolmalaki Z, Zohouri M, et al. Dynamic nucleolar activity in wheat × Aegilops hybrids: evidence of C-genome dominance. Plant Cell Rep. 2017;36(8):1277-1285.
Mirzaghaderi, G., Abdolmalaki, Z., Zohouri, M., Moradi, Z., & Mason, A. S. (2017). Dynamic nucleolar activity in wheat × Aegilops hybrids: evidence of C-genome dominance. Plant Cell Reports, 36(8), 1277-1285. https://doi.org/10.1007/s00299-017-2152-x
Mirzaghaderi G, et al. Dynamic Nucleolar Activity in Wheat × Aegilops Hybrids: Evidence of C-genome Dominance. Plant Cell Rep. 2017;36(8):1277-1285. PubMed PMID: 28456843.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dynamic nucleolar activity in wheat × Aegilops hybrids: evidence of C-genome dominance. AU - Mirzaghaderi,Ghader, AU - Abdolmalaki,Zinat, AU - Zohouri,Mohsen, AU - Moradi,Zeinab, AU - Mason,Annaliese S, Y1 - 2017/04/29/ PY - 2017/03/01/received PY - 2017/04/27/accepted PY - 2017/5/1/pubmed PY - 2018/1/3/medline PY - 2017/5/1/entrez KW - 35S rRNA gene KW - Interspecific hybridization KW - Nucleolar dominance KW - Polyploidization SP - 1277 EP - 1285 JF - Plant cell reports JO - Plant Cell Rep VL - 36 IS - 8 N2 - KEY MESSAGE: NOR loci of C-subgenome are dominant in wheat × Aegilops interspecific hybrids, which may have evolutionary implications for wheat group genome dynamics and evolution. After interspecific hybridisation, some genes are often expressed from only one of the progenitor species, shaping subsequent allopolyploid genome evolution processes. A well-known example is nucleolar dominance, i.e. the formation of cell nucleoli from chromosomes of only one parental species. We studied nucleolar organizing regions (NORs) in diploid Aegilops markgrafii (syn: Ae. caudata; CC), Ae. umbellulata (UU), allotetraploids Aegilops cylindrica (CcCcDcDc) and Ae. triuncialis (CtCtUtUt), synthetic interspecific F1 hybrids between these two allotetraploids and bread wheat (Triticum aestivum, AABBDD) and in F3 generation hybrids with genome composition AABBDDCtCtUtUt using silver staining and fluorescence in situ hybridization (FISH). In Ae. markgrafii (CC), NORs of both 1C and 5C or only 5C chromosome pairs were active in different individual cells, while only NORs on 1U chromosomes were active in Ae. umbellulata (UU). Although all 35S rDNA loci of the Ct subgenome (located on 1Ct and 5Ct) were active in Ae. triuncialis, only one pair (occupying either 1Cc or 5Cc) was active in Ae. cylindrica, depending on the genotype studied. These C-genome expression patterns were transmitted to the F1 and F3 generations. Wheat chromosome NOR activity was variable in Ae. triuncialis × T. aestivum F1 seeds, but silenced by the F3 generation. No effect of maternal or paternal cross direction was observed. These results indicate that C-subgenome NOR loci are dominant in wheat × Aegilops interspecific hybrids, which may have evolutionary implications for wheat group genome dynamics and allopolyploid evolution. SN - 1432-203X UR - https://www.unboundmedicine.com/medline/citation/28456843/Dynamic_nucleolar_activity_in_wheat_×_Aegilops_hybrids:_evidence_of_C_genome_dominance_ L2 - http://dx.doi.org/10.1007/s00299-017-2152-x DB - PRIME DP - Unbound Medicine ER -