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The Nephila clavipes genome highlights the diversity of spider silk genes and their complex expression.
Nat Genet. 2017 Jun; 49(6):895-903.NGen

Abstract

Spider silks are the toughest known biological materials, yet are lightweight and virtually invisible to the human immune system, and they thus have revolutionary potential for medicine and industry. Spider silks are largely composed of spidroins, a unique family of structural proteins. To investigate spidroin genes systematically, we constructed the first genome of an orb-weaving spider: the golden orb-weaver (Nephila clavipes), which builds large webs using an extensive repertoire of silks with diverse physical properties. We cataloged 28 Nephila spidroins, representing all known orb-weaver spidroin types, and identified 394 repeated coding motif variants and higher-order repetitive cassette structures unique to specific spidroins. Characterization of spidroin expression in distinct silk gland types indicates that glands can express multiple spidroin types. We find evidence of an alternatively spliced spidroin, a spidroin expressed only in venom glands, evolutionary mechanisms for spidroin diversification, and non-spidroin genes with expression patterns that suggest roles in silk production.

Authors+Show Affiliations

Department of Systems Pharmacology and Translational Therapeutics, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, Pennsylvania, USA. Department of Genetics, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, Pennsylvania, USA.Department of Systems Pharmacology and Translational Therapeutics, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, Pennsylvania, USA. Institute for Translational Medicine and Therapeutics, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, Pennsylvania, USA.Department of Biology, University of California, Riverside, Riverside, California, USA.Genomics and Computational Biology Graduate Group, University of Pennsylvania, Philadelphia, Pennsylvania, USA.Department of Systems Pharmacology and Translational Therapeutics, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, Pennsylvania, USA. Institute for Translational Medicine and Therapeutics, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, Pennsylvania, USA.Divisions of Perinatal Biology and Immunobiology, Perinatal Institute, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, USA.Biological Institute, Research Centre of the Slovenian Academy of Sciences and Arts, Ljubljana, Slovenia.Department of Biology, University of Vermont, Burlington, Vermont, USA.Department of Biology, University of California, Riverside, Riverside, California, USA.Department of Biology, University of Vermont, Burlington, Vermont, USA.Department of Systems Pharmacology and Translational Therapeutics, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, Pennsylvania, USA. Department of Genetics, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, Pennsylvania, USA. Institute for Translational Medicine and Therapeutics, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, Pennsylvania, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28459453

Citation

Babb, Paul L., et al. "The Nephila Clavipes Genome Highlights the Diversity of Spider Silk Genes and Their Complex Expression." Nature Genetics, vol. 49, no. 6, 2017, pp. 895-903.
Babb PL, Lahens NF, Correa-Garhwal SM, et al. The Nephila clavipes genome highlights the diversity of spider silk genes and their complex expression. Nat Genet. 2017;49(6):895-903.
Babb, P. L., Lahens, N. F., Correa-Garhwal, S. M., Nicholson, D. N., Kim, E. J., Hogenesch, J. B., Kuntner, M., Higgins, L., Hayashi, C. Y., Agnarsson, I., & Voight, B. F. (2017). The Nephila clavipes genome highlights the diversity of spider silk genes and their complex expression. Nature Genetics, 49(6), 895-903. https://doi.org/10.1038/ng.3852
Babb PL, et al. The Nephila Clavipes Genome Highlights the Diversity of Spider Silk Genes and Their Complex Expression. Nat Genet. 2017;49(6):895-903. PubMed PMID: 28459453.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The Nephila clavipes genome highlights the diversity of spider silk genes and their complex expression. AU - Babb,Paul L, AU - Lahens,Nicholas F, AU - Correa-Garhwal,Sandra M, AU - Nicholson,David N, AU - Kim,Eun Ji, AU - Hogenesch,John B, AU - Kuntner,Matjaž, AU - Higgins,Linden, AU - Hayashi,Cheryl Y, AU - Agnarsson,Ingi, AU - Voight,Benjamin F, Y1 - 2017/05/01/ PY - 2016/11/10/received PY - 2017/03/29/accepted PY - 2017/5/2/pubmed PY - 2017/9/13/medline PY - 2017/5/2/entrez SP - 895 EP - 903 JF - Nature genetics JO - Nat. Genet. VL - 49 IS - 6 N2 - Spider silks are the toughest known biological materials, yet are lightweight and virtually invisible to the human immune system, and they thus have revolutionary potential for medicine and industry. Spider silks are largely composed of spidroins, a unique family of structural proteins. To investigate spidroin genes systematically, we constructed the first genome of an orb-weaving spider: the golden orb-weaver (Nephila clavipes), which builds large webs using an extensive repertoire of silks with diverse physical properties. We cataloged 28 Nephila spidroins, representing all known orb-weaver spidroin types, and identified 394 repeated coding motif variants and higher-order repetitive cassette structures unique to specific spidroins. Characterization of spidroin expression in distinct silk gland types indicates that glands can express multiple spidroin types. We find evidence of an alternatively spliced spidroin, a spidroin expressed only in venom glands, evolutionary mechanisms for spidroin diversification, and non-spidroin genes with expression patterns that suggest roles in silk production. SN - 1546-1718 UR - https://www.unboundmedicine.com/medline/citation/28459453/The_Nephila_clavipes_genome_highlights_the_diversity_of_spider_silk_genes_and_their_complex_expression_ L2 - http://dx.doi.org/10.1038/ng.3852 DB - PRIME DP - Unbound Medicine ER -