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Photo-induced and phytomediated synthesis of silver nanoparticles using Derris trifoliata leaf extract and its larvicidal activity against Aedes aegypti.
J Photochem Photobiol B. 2017 Jun; 171:1-8.JP

Abstract

Due to the increased development of resistance of vectors against synthetic insecticides and chemical drugs, plant based insecticides serve as promising biocontrol agents for effective vector control. Green approach for the synthesis of nanoparticles has been attained using environmentally safe, non-toxic plant extracts. The present study was aimed to investigate the potent larvicidal activity of silver nanoparticles (AgNPs) produced by Derris trifoliata leaf extract in relation to the various concentrations of methanol and chloroform extracts for 24h against 3rd and 4th instar larvae of Aedes aegypti. AgNPs were synthesized using D. trifoliata leaf extract as reducing and stabilizing agent. Synthesized AgNPs were characterized by UV-Vis spectroscopy, FTIR spectroscopy, SEM, EDX, XRD and HRTEM. The size of AgNPs as estimated from the full width at half-maximum of (200) peak of silver was 16.13nm, the average crystalline size of the synthesized AgNPs was approximately 20nm, which was correlated with the HRTEM results (20nm). SEM and TEM images have shown the formation of polydispersed nanoparticles with an average size of 20nm. The FTIR spectra of AgNPs exhibited prominent peaks at 2360.7, 1606.2, 1095.6 and 785.9cm-1. The spectral peak observed at 1606.2, assigned to stretching vibration (C=O) in carbonyl compounds characterized by the presence of major constituents of flavonoids and terpenoids. The results obtained in FTIR spectroscopy correlated with the GC-MS analysis of methanol and chloroform extracts and indicates the presence of phytosteroids, flavonoids and terpenoids. The highest larvicidal activity was observed for the synthesized AgNPs against the 3rd instar larvae with LC50 values of 5.87mg/l and LC90 of 12.11mg/l, while against 4th instar larvae these values were7.00 and17.76mg/l respectively. The chloroform extracts also showed increased larvicidal activity than methanol extracts against 3rd instar larvae (LC50=54.42mg/l, LC90=140.83mg/l) and 4th instar larvae (LC50=62.47mg/l, LC90=145.06mg/l) of A. aegypti. Besides, the synthesized AgNPs also exhibited potent antibacterial activity against certain food borne pathogens. These results infer that the biologically synthesized AgNPs and organic solvent extracts have the potential to be used as an excellent eco-friendly approach for vector control against A. aegypti.

Authors+Show Affiliations

Department of Biotechnology, Acharya Nagarjuna University, Guntur, Andhra Pradesh 522510, India.Department of Botany and Microbiology, Acharya Nagarjuna University, Guntur, Andhra Pradesh 522510, India.Department of Microbiology, School of Life Sciences, Pondicherry University, Puducherry 605014, India.Department of Microbiology, School of Life Sciences, Pondicherry University, Puducherry 605014, India.Department of Microbiology, School of Life Sciences, Pondicherry University, Puducherry 605014, India. Electronic address: siddhardha.busi@gmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28460330

Citation

Kumar, Vundru Anil, et al. "Photo-induced and Phytomediated Synthesis of Silver Nanoparticles Using Derris Trifoliata Leaf Extract and Its Larvicidal Activity Against Aedes Aegypti." Journal of Photochemistry and Photobiology. B, Biology, vol. 171, 2017, pp. 1-8.
Kumar VA, Ammani K, Jobina R, et al. Photo-induced and phytomediated synthesis of silver nanoparticles using Derris trifoliata leaf extract and its larvicidal activity against Aedes aegypti. J Photochem Photobiol B. 2017;171:1-8.
Kumar, V. A., Ammani, K., Jobina, R., Subhaswaraj, P., & Siddhardha, B. (2017). Photo-induced and phytomediated synthesis of silver nanoparticles using Derris trifoliata leaf extract and its larvicidal activity against Aedes aegypti. Journal of Photochemistry and Photobiology. B, Biology, 171, 1-8. https://doi.org/10.1016/j.jphotobiol.2017.04.022
Kumar VA, et al. Photo-induced and Phytomediated Synthesis of Silver Nanoparticles Using Derris Trifoliata Leaf Extract and Its Larvicidal Activity Against Aedes Aegypti. J Photochem Photobiol B. 2017;171:1-8. PubMed PMID: 28460330.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Photo-induced and phytomediated synthesis of silver nanoparticles using Derris trifoliata leaf extract and its larvicidal activity against Aedes aegypti. AU - Kumar,Vundru Anil, AU - Ammani,Kandru, AU - Jobina,Rajkumari, AU - Subhaswaraj,Pattnaik, AU - Siddhardha,Busi, Y1 - 2017/04/25/ PY - 2016/03/21/received PY - 2017/04/18/revised PY - 2017/04/19/accepted PY - 2017/5/2/pubmed PY - 2017/9/21/medline PY - 2017/5/2/entrez KW - Aedes aegypti KW - Antibacterial activity KW - Biocontrol KW - Derris trifoliata KW - GC–MS SP - 1 EP - 8 JF - Journal of photochemistry and photobiology. B, Biology JO - J Photochem Photobiol B VL - 171 N2 - Due to the increased development of resistance of vectors against synthetic insecticides and chemical drugs, plant based insecticides serve as promising biocontrol agents for effective vector control. Green approach for the synthesis of nanoparticles has been attained using environmentally safe, non-toxic plant extracts. The present study was aimed to investigate the potent larvicidal activity of silver nanoparticles (AgNPs) produced by Derris trifoliata leaf extract in relation to the various concentrations of methanol and chloroform extracts for 24h against 3rd and 4th instar larvae of Aedes aegypti. AgNPs were synthesized using D. trifoliata leaf extract as reducing and stabilizing agent. Synthesized AgNPs were characterized by UV-Vis spectroscopy, FTIR spectroscopy, SEM, EDX, XRD and HRTEM. The size of AgNPs as estimated from the full width at half-maximum of (200) peak of silver was 16.13nm, the average crystalline size of the synthesized AgNPs was approximately 20nm, which was correlated with the HRTEM results (20nm). SEM and TEM images have shown the formation of polydispersed nanoparticles with an average size of 20nm. The FTIR spectra of AgNPs exhibited prominent peaks at 2360.7, 1606.2, 1095.6 and 785.9cm-1. The spectral peak observed at 1606.2, assigned to stretching vibration (C=O) in carbonyl compounds characterized by the presence of major constituents of flavonoids and terpenoids. The results obtained in FTIR spectroscopy correlated with the GC-MS analysis of methanol and chloroform extracts and indicates the presence of phytosteroids, flavonoids and terpenoids. The highest larvicidal activity was observed for the synthesized AgNPs against the 3rd instar larvae with LC50 values of 5.87mg/l and LC90 of 12.11mg/l, while against 4th instar larvae these values were7.00 and17.76mg/l respectively. The chloroform extracts also showed increased larvicidal activity than methanol extracts against 3rd instar larvae (LC50=54.42mg/l, LC90=140.83mg/l) and 4th instar larvae (LC50=62.47mg/l, LC90=145.06mg/l) of A. aegypti. Besides, the synthesized AgNPs also exhibited potent antibacterial activity against certain food borne pathogens. These results infer that the biologically synthesized AgNPs and organic solvent extracts have the potential to be used as an excellent eco-friendly approach for vector control against A. aegypti. SN - 1873-2682 UR - https://www.unboundmedicine.com/medline/citation/28460330/Photo_induced_and_phytomediated_synthesis_of_silver_nanoparticles_using_Derris_trifoliata_leaf_extract_and_its_larvicidal_activity_against_Aedes_aegypti_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1011-1344(16)30189-0 DB - PRIME DP - Unbound Medicine ER -