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Identification and characterization of the major antifungal substance against Fusarium Sporotrichioides from Chaetomium globosum.
World J Microbiol Biotechnol. 2017 Jun; 33(6):108.WJ

Abstract

Fusarium sporotrichioides, is a common soil-borne plant pathogen causing dry rot of potato in Northeast China. The objective of this study was to identify the main antifungal substances from Chaetomium globosum W7 against F. sporotrichioides. Strain W7 can significantly inhibit F. sporotrichioides without direct contact, suggesting that its antifungal substance was extracellular, and the solubility of this antifungal substance in ethyl acetate was superior to that in water. Acetone was selected as the optimum solvent for the extraction of the metabolites of C. globosum. Metabolites were then separated with thin-layer chromatography. Following antifungal tests on bands, a dark brown band with Rf value of 0.20 was determined as the antifungal substance, and identified as chaetoglobosin A. The antifungal activity test showed that the minimum inhibitory concentration of chaetoglobosin A to F. sporotrichioides was 9.45-10.50 μg/mL, IC50 being 4.344 μg/mL. Chaetoglobosin A also proved to have an excellent preventive effect on potato dry rot caused by F. sporotrichioides. To summarize, chaetoglobosin A was identified as the main active substance of C. globosum to inhibit F. sporotrichioides for the first time, and demonstrated a potential application value in agriculture.

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Authors+Show Affiliations

Jiang C
School of Life Sciences and Technology, Harbin Institute of Technology, Harbin, Heilongjiang, People's Republic of China.
Song J
School of Life Sciences and Technology, Harbin Institute of Technology, Harbin, Heilongjiang, People's Republic of China.
Zhang J
School of Life Sciences and Technology, Harbin Institute of Technology, Harbin, Heilongjiang, People's Republic of China. School of Chemical Engineering and Technology, Harbin Institute of Technology, Harbin, Heilongjiang, People's Republic of China.
Yang Q
School of Life Sciences and Technology, Harbin Institute of Technology, Harbin, Heilongjiang, People's Republic of China. microbio207@gmail.com.

MeSH

Antifungal AgentsChaetomiumChromatography, Thin LayerFusariumIndole AlkaloidsMicrobial Sensitivity TestsSolanum tuberosum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28466302

Citation

Jiang, Cheng, et al. "Identification and Characterization of the Major Antifungal Substance Against Fusarium Sporotrichioides From Chaetomium Globosum." World Journal of Microbiology & Biotechnology, vol. 33, no. 6, 2017, p. 108.
Jiang C, Song J, Zhang J, et al. Identification and characterization of the major antifungal substance against Fusarium Sporotrichioides from Chaetomium globosum. World J Microbiol Biotechnol. 2017;33(6):108.
Jiang, C., Song, J., Zhang, J., & Yang, Q. (2017). Identification and characterization of the major antifungal substance against Fusarium Sporotrichioides from Chaetomium globosum. World Journal of Microbiology & Biotechnology, 33(6), 108. https://doi.org/10.1007/s11274-017-2274-x
Jiang C, et al. Identification and Characterization of the Major Antifungal Substance Against Fusarium Sporotrichioides From Chaetomium Globosum. World J Microbiol Biotechnol. 2017;33(6):108. PubMed PMID: 28466302.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and characterization of the major antifungal substance against Fusarium Sporotrichioides from Chaetomium globosum. AU - Jiang,Cheng, AU - Song,Jinzhu, AU - Zhang,Junzheng, AU - Yang,Qian, Y1 - 2017/05/02/ PY - 2017/02/27/received PY - 2017/04/26/accepted PY - 2017/5/4/entrez PY - 2017/5/4/pubmed PY - 2017/9/21/medline KW - Antifungal KW - Chaetoglobosin A KW - Chaetomium globosum KW - Fusarium sporotrichioides SP - 108 EP - 108 JF - World journal of microbiology & biotechnology JO - World J Microbiol Biotechnol VL - 33 IS - 6 N2 - Fusarium sporotrichioides, is a common soil-borne plant pathogen causing dry rot of potato in Northeast China. The objective of this study was to identify the main antifungal substances from Chaetomium globosum W7 against F. sporotrichioides. Strain W7 can significantly inhibit F. sporotrichioides without direct contact, suggesting that its antifungal substance was extracellular, and the solubility of this antifungal substance in ethyl acetate was superior to that in water. Acetone was selected as the optimum solvent for the extraction of the metabolites of C. globosum. Metabolites were then separated with thin-layer chromatography. Following antifungal tests on bands, a dark brown band with Rf value of 0.20 was determined as the antifungal substance, and identified as chaetoglobosin A. The antifungal activity test showed that the minimum inhibitory concentration of chaetoglobosin A to F. sporotrichioides was 9.45-10.50 μg/mL, IC50 being 4.344 μg/mL. Chaetoglobosin A also proved to have an excellent preventive effect on potato dry rot caused by F. sporotrichioides. To summarize, chaetoglobosin A was identified as the main active substance of C. globosum to inhibit F. sporotrichioides for the first time, and demonstrated a potential application value in agriculture. SN - 1573-0972 UR - https://www.unboundmedicine.com/medline/citation/28466302/Identification_and_characterization_of_the_major_antifungal_substance_against_Fusarium_Sporotrichioides_from_Chaetomium_globosum_ L2 - https://dx.doi.org/10.1007/s11274-017-2274-x DB - PRIME DP - Unbound Medicine ER -