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Chemical Profile and Antioxidant, Anti-Inflammatory, Antimutagenic and Antimicrobial Activities of Geopropolis from the Stingless Bee Melipona orbignyi.
Int J Mol Sci. 2017 May 03; 18(5)IJ

Abstract

Geopropolis is a resin mixed with mud, produced only by stingless bees. Despite being popularly known for its medicinal properties, few scientific studies have proven its biological activities. In this context, the objective of this study was to determine the chemical composition and antioxidant, anti-inflammatory, antimutagenic and antimicrobial activities of the Melipona orbignyi geopropolis. The hydroalcoholic extract of geopropolis (HEGP) was prepared and its chemical composition determined by high performance liquid chromatography coupled to diode array detector and mass spectrometry (HPLC-DAD-MS). The antioxidant activity was determined by the capture of free radicals and inhibition of lipid peroxidation in human erythrocytes. The anti-inflammatory activity was evaluated by the inhibition of the hyaluronidase enzyme and the antimutagenic action was investigated in Saccharomyces cerevisiae colonies. The antimicrobial activities were determined against bacteria and yeasts, isolated from reference strains and hospital origin. The chemical composition of HEGP included flavonoids, derivatives of glycosylated phenolic acids and terpenoids. HEGP showed high antioxidant activity, it inhibited the activity of the inflammatory enzyme hyaluronidase and reduced the mutagenic effects in S. cerevisiae. In relation to the antimicrobial activity, it promoted the death of all microorganisms evaluated. In conclusion, this study reveals for the first time the chemical composition of the HEGP of M. orbignyi and demonstrates its pharmacological properties.

Authors+Show Affiliations

Research group on Biotechnology and Bioprospecting Applied to Metabolism (GEBBAM), Federal University of Grande Dourados, Rodovia Dourados Itahum, Km 12, 79804-970 Dourados, MS, Brazil. helderspk@gmail.com.Research group on Biotechnology and Bioprospecting Applied to Metabolism (GEBBAM), Federal University of Grande Dourados, Rodovia Dourados Itahum, Km 12, 79804-970 Dourados, MS, Brazil. jcampos_bio@yahoo.com.br.Research group on Biotechnology and Bioprospecting Applied to Metabolism (GEBBAM), Federal University of Grande Dourados, Rodovia Dourados Itahum, Km 12, 79804-970 Dourados, MS, Brazil. cinty.santos94@gmail.com.Research group on Biotechnology and Bioprospecting Applied to Metabolism (GEBBAM), Federal University of Grande Dourados, Rodovia Dourados Itahum, Km 12, 79804-970 Dourados, MS, Brazil. josebalestieri@ufgd.edu.br.Laboratory of Natural Products and Mass Spectrometry, Federal University of Mato Grosso do Sul, Cidade Universitária, 79070-900 Campo Grande, MS, Brazil. denisebrentan@gmail.com.Laboratory of Natural Products and Mass Spectrometry, Federal University of Mato Grosso do Sul, Cidade Universitária, 79070-900 Campo Grande, MS, Brazil. carloscarollo@gmail.com.Research group on Biotechnology and Bioprospecting Applied to Metabolism (GEBBAM), Federal University of Grande Dourados, Rodovia Dourados Itahum, Km 12, 79804-970 Dourados, MS, Brazil. kelypicoli@gmail.com.Polytechnic Institute of Bragança, Agricultural College of Bragança, Campus Santa Apolónia, E 5301-855 Bragança, Portugal. leticia@ipb.pt. Centre of Molecular and Environmental Biology, Biology Department, Minho University, Campus de Gualtar, 4710-057 Braga, Portugal. leticia@ipb.pt.Research group on Biotechnology and Bioprospecting Applied to Metabolism (GEBBAM), Federal University of Grande Dourados, Rodovia Dourados Itahum, Km 12, 79804-970 Dourados, MS, Brazil. edsonsantosphd@gmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28467350

Citation

Santos, Helder Freitas Dos, et al. "Chemical Profile and Antioxidant, Anti-Inflammatory, Antimutagenic and Antimicrobial Activities of Geopropolis From the Stingless Bee Melipona Orbignyi." International Journal of Molecular Sciences, vol. 18, no. 5, 2017.
Santos HFD, Campos JF, Santos CMD, et al. Chemical Profile and Antioxidant, Anti-Inflammatory, Antimutagenic and Antimicrobial Activities of Geopropolis from the Stingless Bee Melipona orbignyi. Int J Mol Sci. 2017;18(5).
Santos, H. F. D., Campos, J. F., Santos, C. M. D., Balestieri, J. B. P., Silva, D. B., Carollo, C. A., de Picoli Souza, K., Estevinho, L. M., & Dos Santos, E. L. (2017). Chemical Profile and Antioxidant, Anti-Inflammatory, Antimutagenic and Antimicrobial Activities of Geopropolis from the Stingless Bee Melipona orbignyi. International Journal of Molecular Sciences, 18(5). https://doi.org/10.3390/ijms18050953
Santos HFD, et al. Chemical Profile and Antioxidant, Anti-Inflammatory, Antimutagenic and Antimicrobial Activities of Geopropolis From the Stingless Bee Melipona Orbignyi. Int J Mol Sci. 2017 May 3;18(5) PubMed PMID: 28467350.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chemical Profile and Antioxidant, Anti-Inflammatory, Antimutagenic and Antimicrobial Activities of Geopropolis from the Stingless Bee Melipona orbignyi. AU - Santos,Helder Freitas Dos, AU - Campos,Jaqueline Ferreira, AU - Santos,Cintia Miranda Dos, AU - Balestieri,José Benedito Perrella, AU - Silva,Denise Brentan, AU - Carollo,Carlos Alexandre, AU - de Picoli Souza,Kely, AU - Estevinho,Leticia Miranda, AU - Dos Santos,Edson Lucas, Y1 - 2017/05/03/ PY - 2017/04/04/received PY - 2017/04/27/accepted PY - 2017/5/4/entrez PY - 2017/5/4/pubmed PY - 2018/2/22/medline KW - HPLC-DAD-MS 1 KW - hyaluronidase 4 KW - lipid peroxidation 3 KW - microorganisms 6 KW - mutation 5 KW - phenolic compounds 2 JF - International journal of molecular sciences JO - Int J Mol Sci VL - 18 IS - 5 N2 - Geopropolis is a resin mixed with mud, produced only by stingless bees. Despite being popularly known for its medicinal properties, few scientific studies have proven its biological activities. In this context, the objective of this study was to determine the chemical composition and antioxidant, anti-inflammatory, antimutagenic and antimicrobial activities of the Melipona orbignyi geopropolis. The hydroalcoholic extract of geopropolis (HEGP) was prepared and its chemical composition determined by high performance liquid chromatography coupled to diode array detector and mass spectrometry (HPLC-DAD-MS). The antioxidant activity was determined by the capture of free radicals and inhibition of lipid peroxidation in human erythrocytes. The anti-inflammatory activity was evaluated by the inhibition of the hyaluronidase enzyme and the antimutagenic action was investigated in Saccharomyces cerevisiae colonies. The antimicrobial activities were determined against bacteria and yeasts, isolated from reference strains and hospital origin. The chemical composition of HEGP included flavonoids, derivatives of glycosylated phenolic acids and terpenoids. HEGP showed high antioxidant activity, it inhibited the activity of the inflammatory enzyme hyaluronidase and reduced the mutagenic effects in S. cerevisiae. In relation to the antimicrobial activity, it promoted the death of all microorganisms evaluated. In conclusion, this study reveals for the first time the chemical composition of the HEGP of M. orbignyi and demonstrates its pharmacological properties. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/28467350/Chemical_Profile_and_Antioxidant_Anti_Inflammatory_Antimutagenic_and_Antimicrobial_Activities_of_Geopropolis_from_the_Stingless_Bee_Melipona_orbignyi_ L2 - http://www.mdpi.com/resolver?pii=ijms18050953 DB - PRIME DP - Unbound Medicine ER -