Tags

Type your tag names separated by a space and hit enter

Atractylenolide-I Protects Human SH-SY5Y Cells from 1-Methyl-4-Phenylpyridinium-Induced Apoptotic Cell Death.
Int J Mol Sci. 2017 May 08; 18(5)IJ

Abstract

Oxidative stress and apoptosis are the major mechanisms that induce dopaminergic cell death. Our study investigates the protective effects of atractylenolide-I (ATR-I) on 1-methyl-4-phenylpyridinium (MPP⁺)-induced cytotoxicity in human dopaminergic SH-SY5Y cells, as well as its underlying mechanism. Our experimental data indicates that ATR-I significantly inhibits the loss of cell viability induced by MPP⁺ in SH-SY5Y cells. To further unravel the mechanism, we examined the effect of ATR-I on MPP⁺-induced apoptotic cell death characterized by an increase in the Bax/Bcl-2 mRNA ratio, the release of cytochrome-c, and the activation of caspase-3 leading to elevated levels of cleaved poly(ADP-ribose) polymerase (PARP) resulting in SH-SY5Y cell death. Our results demonstrated that ATR-I decreases the level of pro-apoptotic proteins induced by MPP⁺ and also restored Bax/Bcl-2 mRNA levels, which are critical for inducing apoptosis. In addition, ATR-I demonstrated a significant increase in the protein expression of heme-oxygenase in MPP⁺-treated SH-SY5Y cells. These results suggest that the pharmacological effect of ATR-I may be, at least in part, caused by the reduction in pro-apoptotic signals and also by induction of anti-oxidant protein.

Authors+Show Affiliations

Department of Biotechnology, College of Biomedical and Health Science, Konkuk University, Chungju 380-701, Korea. sandeepbcp@gmail.com.Department of Biotechnology, College of Biomedical and Health Science, Konkuk University, Chungju 380-701, Korea. choidk@kku.ac.kr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28481321

Citation

More, Sandeep Vasant, and Dong-Kug Choi. "Atractylenolide-I Protects Human SH-SY5Y Cells From 1-Methyl-4-Phenylpyridinium-Induced Apoptotic Cell Death." International Journal of Molecular Sciences, vol. 18, no. 5, 2017.
More SV, Choi DK. Atractylenolide-I Protects Human SH-SY5Y Cells from 1-Methyl-4-Phenylpyridinium-Induced Apoptotic Cell Death. Int J Mol Sci. 2017;18(5).
More, S. V., & Choi, D. K. (2017). Atractylenolide-I Protects Human SH-SY5Y Cells from 1-Methyl-4-Phenylpyridinium-Induced Apoptotic Cell Death. International Journal of Molecular Sciences, 18(5). https://doi.org/10.3390/ijms18051012
More SV, Choi DK. Atractylenolide-I Protects Human SH-SY5Y Cells From 1-Methyl-4-Phenylpyridinium-Induced Apoptotic Cell Death. Int J Mol Sci. 2017 May 8;18(5) PubMed PMID: 28481321.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Atractylenolide-I Protects Human SH-SY5Y Cells from 1-Methyl-4-Phenylpyridinium-Induced Apoptotic Cell Death. AU - More,Sandeep Vasant, AU - Choi,Dong-Kug, Y1 - 2017/05/08/ PY - 2017/01/24/received PY - 2017/04/21/revised PY - 2017/05/03/accepted PY - 2017/5/9/entrez PY - 2017/5/10/pubmed PY - 2018/2/9/medline KW - MPP+ KW - Parkinson’s disease KW - apoptosis KW - atractylenolide-I KW - neuroprotection JF - International journal of molecular sciences JO - Int J Mol Sci VL - 18 IS - 5 N2 - Oxidative stress and apoptosis are the major mechanisms that induce dopaminergic cell death. Our study investigates the protective effects of atractylenolide-I (ATR-I) on 1-methyl-4-phenylpyridinium (MPP⁺)-induced cytotoxicity in human dopaminergic SH-SY5Y cells, as well as its underlying mechanism. Our experimental data indicates that ATR-I significantly inhibits the loss of cell viability induced by MPP⁺ in SH-SY5Y cells. To further unravel the mechanism, we examined the effect of ATR-I on MPP⁺-induced apoptotic cell death characterized by an increase in the Bax/Bcl-2 mRNA ratio, the release of cytochrome-c, and the activation of caspase-3 leading to elevated levels of cleaved poly(ADP-ribose) polymerase (PARP) resulting in SH-SY5Y cell death. Our results demonstrated that ATR-I decreases the level of pro-apoptotic proteins induced by MPP⁺ and also restored Bax/Bcl-2 mRNA levels, which are critical for inducing apoptosis. In addition, ATR-I demonstrated a significant increase in the protein expression of heme-oxygenase in MPP⁺-treated SH-SY5Y cells. These results suggest that the pharmacological effect of ATR-I may be, at least in part, caused by the reduction in pro-apoptotic signals and also by induction of anti-oxidant protein. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/28481321/Atractylenolide_I_Protects_Human_SH_SY5Y_Cells_from_1_Methyl_4_Phenylpyridinium_Induced_Apoptotic_Cell_Death_ L2 - https://www.mdpi.com/resolver?pii=ijms18051012 DB - PRIME DP - Unbound Medicine ER -