Tags

Type your tag names separated by a space and hit enter

Dihydromyricetin induces apoptosis and cytoprotective autophagy through ROS-NF-κB signalling in human melanoma cells.
Free Radic Res. 2017 May; 51(5):517-528.FR

Abstract

Dihydromyricetin (DHM), a Rattan tea extract, has recently been shown to have anti-cancer activity in mammalian cells. In this study, we investigated the effect of DHM on human melanoma cells. Apart from induction of apoptosis, we demonstrated that DHM induced an autophagic response. Moreover, pharmacological inhibition or genetic blockade of autophagy enhanced DHM-induced cell death and apoptosis, indicating the cytoprotective role of autophagy in DHM-treated human melanoma cells. Further study suggested that the nuclear factor kappa B (NF-κB) signalling pathway was involved in DHM-induced autophagy. Moreover, N-acetyl-cysteine (NAC), an ROS scavenger, abrogated the effects of DHM on NF-κB-dependent autophagy. Taken together, this evidence demonstrates that a strategy of blocking ROS-NF-κB-dependent autophagy to enhance the activity of DHM warrants further attention for the treatment of human melanoma.

Authors+Show Affiliations

a Department of Neurosurgery , The Central Hospital of ShaoYang , Shaoyang , PR China.a Department of Neurosurgery , The Central Hospital of ShaoYang , Shaoyang , PR China.a Department of Neurosurgery , The Central Hospital of ShaoYang , Shaoyang , PR China.b Department of Neurosurgery , The Second Xiangya Hospital of Central South University , Changsha , PR China.a Department of Neurosurgery , The Central Hospital of ShaoYang , Shaoyang , PR China.a Department of Neurosurgery , The Central Hospital of ShaoYang , Shaoyang , PR China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28482716

Citation

Zhou, Ding-Zhou, et al. "Dihydromyricetin Induces Apoptosis and Cytoprotective Autophagy Through ROS-NF-κB Signalling in Human Melanoma Cells." Free Radical Research, vol. 51, no. 5, 2017, pp. 517-528.
Zhou DZ, Sun HY, Yue JQ, et al. Dihydromyricetin induces apoptosis and cytoprotective autophagy through ROS-NF-κB signalling in human melanoma cells. Free Radic Res. 2017;51(5):517-528.
Zhou, D. Z., Sun, H. Y., Yue, J. Q., Peng, Y., Chen, Y. M., & Zhong, Z. J. (2017). Dihydromyricetin induces apoptosis and cytoprotective autophagy through ROS-NF-κB signalling in human melanoma cells. Free Radical Research, 51(5), 517-528. https://doi.org/10.1080/10715762.2017.1328552
Zhou DZ, et al. Dihydromyricetin Induces Apoptosis and Cytoprotective Autophagy Through ROS-NF-κB Signalling in Human Melanoma Cells. Free Radic Res. 2017;51(5):517-528. PubMed PMID: 28482716.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dihydromyricetin induces apoptosis and cytoprotective autophagy through ROS-NF-κB signalling in human melanoma cells. AU - Zhou,Ding-Zhou, AU - Sun,Hai-Ying, AU - Yue,Jing-Qi, AU - Peng,Yong, AU - Chen,Yi-Min, AU - Zhong,Zhi-Jian, PY - 2017/5/10/pubmed PY - 2018/3/13/medline PY - 2017/5/10/entrez KW - Dihydromyricetin KW - NF-κB KW - apoptosis KW - autophagy KW - melanoma KW - reactive oxygen species SP - 517 EP - 528 JF - Free radical research JO - Free Radic. Res. VL - 51 IS - 5 N2 - Dihydromyricetin (DHM), a Rattan tea extract, has recently been shown to have anti-cancer activity in mammalian cells. In this study, we investigated the effect of DHM on human melanoma cells. Apart from induction of apoptosis, we demonstrated that DHM induced an autophagic response. Moreover, pharmacological inhibition or genetic blockade of autophagy enhanced DHM-induced cell death and apoptosis, indicating the cytoprotective role of autophagy in DHM-treated human melanoma cells. Further study suggested that the nuclear factor kappa B (NF-κB) signalling pathway was involved in DHM-induced autophagy. Moreover, N-acetyl-cysteine (NAC), an ROS scavenger, abrogated the effects of DHM on NF-κB-dependent autophagy. Taken together, this evidence demonstrates that a strategy of blocking ROS-NF-κB-dependent autophagy to enhance the activity of DHM warrants further attention for the treatment of human melanoma. SN - 1029-2470 UR - https://www.unboundmedicine.com/medline/citation/28482716/Dihydromyricetin_induces_apoptosis_and_cytoprotective_autophagy_through_ROS_NF_κB_signalling_in_human_melanoma_cells_ L2 - http://www.tandfonline.com/doi/full/10.1080/10715762.2017.1328552 DB - PRIME DP - Unbound Medicine ER -