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ES7, encoding a ferredoxin-dependent glutamate synthase, functions in nitrogen metabolism and impacts leaf senescence in rice.
Plant Sci. 2017 Jun; 259:24-34.PS

Abstract

Glutamate synthase (GOGAT) is a key enzyme for nitrogen metabolism and ammonium assimilation in plants. In this study, an early senescence 7 (es7) mutant was identified and characterized. The leaves of the es7 mutant begin to senesce at the tillering stage about 60day after sowing, and become increasingly senescent as the plants develop at the heading stage. When es7 plants are grown under photorespiration-suppressed conditions (high CO2), the senescence phenotype and chlorophyll content are rescued. qRT-PCR analysis showed that senescence- associated genes were up-regulated significantly in es7. A map-based cloning strategy was used to identify ES7, which encodes a ferredoxin-dependent glutamate synthase (Fd-GOGAT). ES7 was expressed constitutively, and the ES7 protein was localized in chloroplast. qRT-PCR analysis indicated that several genes related to nitrogen metabolism were differentially expressed in es7. Further, we also demonstrated that chlorophyll synthesis-associated genes were significantly down-regulated in es7. In addition, when seedlings are grown under increasing nitrogen concentrations (NH4NO3) for 15days, the contents of chlorophyll a, chlorophyll b and total chlorophyll were significantly lower in es7. Our results demonstrated that ES7 is involved in nitrogen metabolism, effects chlorophyll synthesis, and may also associated with photorespiration, impacting leaf senescence in rice.

Authors+Show Affiliations

State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, Zhejiang 310006, China; Key Laboratory for Zhejiang Super Rice Research, China National Rice Research Institute, Hangzhou, Zhejiang 310006, China. Electronic address: bizhen925@sina.com.State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, Zhejiang 310006, China; Key Laboratory for Zhejiang Super Rice Research, China National Rice Research Institute, Hangzhou, Zhejiang 310006, China. Electronic address: zyxrice@163.com.State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, Zhejiang 310006, China; Key Laboratory for Zhejiang Super Rice Research, China National Rice Research Institute, Hangzhou, Zhejiang 310006, China. Electronic address: wuweixun@caas.cn.State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, Zhejiang 310006, China; Key Laboratory for Zhejiang Super Rice Research, China National Rice Research Institute, Hangzhou, Zhejiang 310006, China. Electronic address: zhanxiaodeng@hotmail.com.State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, Zhejiang 310006, China; Key Laboratory for Zhejiang Super Rice Research, China National Rice Research Institute, Hangzhou, Zhejiang 310006, China. Electronic address: yuning02@163.com.State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, Zhejiang 310006, China; Key Laboratory for Zhejiang Super Rice Research, China National Rice Research Institute, Hangzhou, Zhejiang 310006, China. Electronic address: xutingting9063@126.com.State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, Zhejiang 310006, China; Key Laboratory for Zhejiang Super Rice Research, China National Rice Research Institute, Hangzhou, Zhejiang 310006, China. Electronic address: liuqunen202@163.com.State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, Zhejiang 310006, China; Key Laboratory for Zhejiang Super Rice Research, China National Rice Research Institute, Hangzhou, Zhejiang 310006, China. Electronic address: lizhihpp@163.com.State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, Zhejiang 310006, China; Key Laboratory for Zhejiang Super Rice Research, China National Rice Research Institute, Hangzhou, Zhejiang 310006, China. Electronic address: xihongshen@126.com.State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, Zhejiang 310006, China; Key Laboratory for Zhejiang Super Rice Research, China National Rice Research Institute, Hangzhou, Zhejiang 310006, China. Electronic address: cdb840925@163.com.State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, Zhejiang 310006, China; Key Laboratory for Zhejiang Super Rice Research, China National Rice Research Institute, Hangzhou, Zhejiang 310006, China. Electronic address: shcheng@mail.hz.zj.cn.State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, Zhejiang 310006, China; Key Laboratory for Zhejiang Super Rice Research, China National Rice Research Institute, Hangzhou, Zhejiang 310006, China. Electronic address: caolycgf@mail.hz.zj.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28483051

Citation

Bi, Zhenzhen, et al. "ES7, Encoding a Ferredoxin-dependent Glutamate Synthase, Functions in Nitrogen Metabolism and Impacts Leaf Senescence in Rice." Plant Science : an International Journal of Experimental Plant Biology, vol. 259, 2017, pp. 24-34.
Bi Z, Zhang Y, Wu W, et al. ES7, encoding a ferredoxin-dependent glutamate synthase, functions in nitrogen metabolism and impacts leaf senescence in rice. Plant Sci. 2017;259:24-34.
Bi, Z., Zhang, Y., Wu, W., Zhan, X., Yu, N., Xu, T., Liu, Q., Li, Z., Shen, X., Chen, D., Cheng, S., & Cao, L. (2017). ES7, encoding a ferredoxin-dependent glutamate synthase, functions in nitrogen metabolism and impacts leaf senescence in rice. Plant Science : an International Journal of Experimental Plant Biology, 259, 24-34. https://doi.org/10.1016/j.plantsci.2017.03.003
Bi Z, et al. ES7, Encoding a Ferredoxin-dependent Glutamate Synthase, Functions in Nitrogen Metabolism and Impacts Leaf Senescence in Rice. Plant Sci. 2017;259:24-34. PubMed PMID: 28483051.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - ES7, encoding a ferredoxin-dependent glutamate synthase, functions in nitrogen metabolism and impacts leaf senescence in rice. AU - Bi,Zhenzhen, AU - Zhang,Yingxin, AU - Wu,Weixun, AU - Zhan,Xiaodeng, AU - Yu,Ning, AU - Xu,Tingting, AU - Liu,Qunen, AU - Li,Zhi, AU - Shen,Xihong, AU - Chen,Daibo, AU - Cheng,Shihua, AU - Cao,Liyong, Y1 - 2017/03/14/ PY - 2016/11/16/received PY - 2017/03/01/revised PY - 2017/03/08/accepted PY - 2017/5/10/entrez PY - 2017/5/10/pubmed PY - 2017/12/30/medline KW - Chlorophyll metabolism KW - ES7 KW - Ferredoxin-dependent glutamate synthase KW - Leaf senescence KW - Nitrogen metabolism KW - Photorespiration SP - 24 EP - 34 JF - Plant science : an international journal of experimental plant biology JO - Plant Sci VL - 259 N2 - Glutamate synthase (GOGAT) is a key enzyme for nitrogen metabolism and ammonium assimilation in plants. In this study, an early senescence 7 (es7) mutant was identified and characterized. The leaves of the es7 mutant begin to senesce at the tillering stage about 60day after sowing, and become increasingly senescent as the plants develop at the heading stage. When es7 plants are grown under photorespiration-suppressed conditions (high CO2), the senescence phenotype and chlorophyll content are rescued. qRT-PCR analysis showed that senescence- associated genes were up-regulated significantly in es7. A map-based cloning strategy was used to identify ES7, which encodes a ferredoxin-dependent glutamate synthase (Fd-GOGAT). ES7 was expressed constitutively, and the ES7 protein was localized in chloroplast. qRT-PCR analysis indicated that several genes related to nitrogen metabolism were differentially expressed in es7. Further, we also demonstrated that chlorophyll synthesis-associated genes were significantly down-regulated in es7. In addition, when seedlings are grown under increasing nitrogen concentrations (NH4NO3) for 15days, the contents of chlorophyll a, chlorophyll b and total chlorophyll were significantly lower in es7. Our results demonstrated that ES7 is involved in nitrogen metabolism, effects chlorophyll synthesis, and may also associated with photorespiration, impacting leaf senescence in rice. SN - 1873-2259 UR - https://www.unboundmedicine.com/medline/citation/28483051/ES7_encoding_a_ferredoxin_dependent_glutamate_synthase_functions_in_nitrogen_metabolism_and_impacts_leaf_senescence_in_rice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168-9452(16)30654-9 DB - PRIME DP - Unbound Medicine ER -