Tags

Type your tag names separated by a space and hit enter

Identification of aberrantly expressed long non-coding RNAs in stomach adenocarcinoma.
Oncotarget. 2017 Jul 25; 8(30):49201-49216.O

Abstract

AIM

Stomach adenocarcinoma (STAD) is a common malignancy worldwide. This study aimed to identify the aberrantly expressed long non-coding RNAs (lncRNAs) in STAD.

RESULTS

Total of 74 DElncRNAs and 449 DEmRNAs were identified in STAD compared with paired non-tumor tissues. The DElncRNA/DEmRNA co-expression network was constructed, which covered 519 nodes and 2993 edges. The qRT-PCR validation results of DElncRNAs were consistent with our bioinformatics analysis based on RNA-sequencing. The DEmRNAs co-expressed with DElncRNAs were significantly enriched in gastric acid secretion, complement and coagulation cascades, pancreatic secretion, cytokine-cytokine receptor interaction and Jak-STAT signaling pathway. The expression levels of the nine candidate DElncRNAs in TCGA database were compatible with our RNA-sequencing. FEZF1-AS1, HOTAIR and LINC01234 had the potential diagnosis value for STAD.

MATERIALS AND METHODS

The lncRNA and mRNA expression profile of 3 STAD tissues and 3 matched adjacent non-tumor tissues was obtained through high-throughput RNA-sequencing. Differentially expressed lncRNAs/mRNAs (DElncRNAs/DEmRNAs) were identified in STAD. DElncRNA/DEmRNA co-expression network construction, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were conducted to predict the biological functions of DElncRNAs. Quantitative real-time polymerase chain reaction (qRT-PCR) was subjected to validate the expression levels of DEmRNAs and DElncRNAs. Moreover, the expression of DElncRNAs was validated through The Cancer Genome Atlas (TCGA) database. The diagnosis value of candidate DElncRNAs was accessed by receiver operating characteristic (ROC) analysis.

CONCLUSIONS

Our work might provide useful information for exploring the tumorigenesis mechanism of STAD and pave the road for identification of diagnostic biomarkers in STAD.

Authors+Show Affiliations

Department of General Surgery, The Fourth Affiliated Hospital of Hebei Medical University, Hebei, China.Department of General Surgery, The Fourth Affiliated Hospital of Hebei Medical University, Hebei, China.Department of General Surgery, The Fourth Affiliated Hospital of Hebei Medical University, Hebei, China.Department of General Surgery, The Fourth Affiliated Hospital of Hebei Medical University, Hebei, China.Department of General Surgery, The Fourth Affiliated Hospital of Hebei Medical University, Hebei, China.Department of General Surgery, The Fourth Affiliated Hospital of Hebei Medical University, Hebei, China.Department of General Surgery, The Fourth Affiliated Hospital of Hebei Medical University, Hebei, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28484081

Citation

Gu, Jianbin, et al. "Identification of Aberrantly Expressed Long Non-coding RNAs in Stomach Adenocarcinoma." Oncotarget, vol. 8, no. 30, 2017, pp. 49201-49216.
Gu J, Li Y, Fan L, et al. Identification of aberrantly expressed long non-coding RNAs in stomach adenocarcinoma. Oncotarget. 2017;8(30):49201-49216.
Gu, J., Li, Y., Fan, L., Zhao, Q., Tan, B., Hua, K., & Wu, G. (2017). Identification of aberrantly expressed long non-coding RNAs in stomach adenocarcinoma. Oncotarget, 8(30), 49201-49216. https://doi.org/10.18632/oncotarget.17329
Gu J, et al. Identification of Aberrantly Expressed Long Non-coding RNAs in Stomach Adenocarcinoma. Oncotarget. 2017 Jul 25;8(30):49201-49216. PubMed PMID: 28484081.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of aberrantly expressed long non-coding RNAs in stomach adenocarcinoma. AU - Gu,Jianbin, AU - Li,Yong, AU - Fan,Liqiao, AU - Zhao,Qun, AU - Tan,Bibo, AU - Hua,Kelei, AU - Wu,Guobin, PY - 2016/09/08/received PY - 2017/04/11/accepted PY - 2017/5/10/pubmed PY - 2018/4/24/medline PY - 2017/5/10/entrez KW - co-expression KW - expression profile KW - long non-coding RNA KW - stomach adenocarcinoma KW - tumorigenesis SP - 49201 EP - 49216 JF - Oncotarget JO - Oncotarget VL - 8 IS - 30 N2 - AIM: Stomach adenocarcinoma (STAD) is a common malignancy worldwide. This study aimed to identify the aberrantly expressed long non-coding RNAs (lncRNAs) in STAD. RESULTS: Total of 74 DElncRNAs and 449 DEmRNAs were identified in STAD compared with paired non-tumor tissues. The DElncRNA/DEmRNA co-expression network was constructed, which covered 519 nodes and 2993 edges. The qRT-PCR validation results of DElncRNAs were consistent with our bioinformatics analysis based on RNA-sequencing. The DEmRNAs co-expressed with DElncRNAs were significantly enriched in gastric acid secretion, complement and coagulation cascades, pancreatic secretion, cytokine-cytokine receptor interaction and Jak-STAT signaling pathway. The expression levels of the nine candidate DElncRNAs in TCGA database were compatible with our RNA-sequencing. FEZF1-AS1, HOTAIR and LINC01234 had the potential diagnosis value for STAD. MATERIALS AND METHODS: The lncRNA and mRNA expression profile of 3 STAD tissues and 3 matched adjacent non-tumor tissues was obtained through high-throughput RNA-sequencing. Differentially expressed lncRNAs/mRNAs (DElncRNAs/DEmRNAs) were identified in STAD. DElncRNA/DEmRNA co-expression network construction, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were conducted to predict the biological functions of DElncRNAs. Quantitative real-time polymerase chain reaction (qRT-PCR) was subjected to validate the expression levels of DEmRNAs and DElncRNAs. Moreover, the expression of DElncRNAs was validated through The Cancer Genome Atlas (TCGA) database. The diagnosis value of candidate DElncRNAs was accessed by receiver operating characteristic (ROC) analysis. CONCLUSIONS: Our work might provide useful information for exploring the tumorigenesis mechanism of STAD and pave the road for identification of diagnostic biomarkers in STAD. SN - 1949-2553 UR - https://www.unboundmedicine.com/medline/citation/28484081/Identification_of_aberrantly_expressed_long_non_coding_RNAs_in_stomach_adenocarcinoma_ L2 - https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.17329 DB - PRIME DP - Unbound Medicine ER -