TY - JOUR T1 - An Efficient Method for the Preparative Isolation and Purification of Flavonoids from Leaves of Crataegus pinnatifida by HSCCC and Pre-HPLC. AU - Wen,Lei, AU - Lin,Yunliang, AU - Lv,Ruimin, AU - Yan,Huijiao, AU - Yu,Jinqian, AU - Zhao,Hengqiang, AU - Wang,Xiao, AU - Wang,Daijie, Y1 - 2017/05/09/ PY - 2017/03/08/received PY - 2017/04/24/revised PY - 2017/05/03/accepted PY - 2017/5/10/entrez PY - 2017/5/10/pubmed PY - 2018/2/24/medline KW - Crataegus pinnatifida leaves KW - HSCCC and pre-HPLC combination KW - extrusion mode KW - flavonoids JF - Molecules (Basel, Switzerland) JO - Molecules VL - 22 IS - 5 N2 - In this work, flavonoid fraction from the leaves of Crataegus pinnatifida was separated into its seven main constituents using a combination of HSCCC coupled with pre-HPLC. In the first step, the total flavonoid extract was subjected to HSCCC with a two-solvent system of chloroform/methanol/water/n-butanol (4:3:2:1.5, v/v), yielding four pure compounds, namely (-)-epicatechin (1), quercetin-3-O-(2,6-di-α-l-rhamnopyranosyl)-β-d-galactopyranoside (2), 4''-O-glucosylvitexin (3) and 2''-O-rhamnosylvitexin (4) as well as a mixture of three further flavonoids. An extrusion mode was used to rapidly separate quercetin-3-O-(2,6-di-α-l-rhamnopyranosyl)-β-d-galactopyranoside with a big KD-value. In the second step, the mixture that resulted from HSCCC was separated by pre-HPLC, resulting in three pure compounds including: vitexin (5), hyperoside (6) and isoquercitrin (7). The purities of the isolated compounds were established to be over 98%, as determined by HPLC. The structures of these seven flavonoids were elucidated by ESI-MS and NMR spectroscopic analyses. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/28486427/An_Efficient_Method_for_the_Preparative_Isolation_and_Purification_of_Flavonoids_from_Leaves_of_Crataegus_pinnatifida_by_HSCCC_and_Pre_HPLC_ DB - PRIME DP - Unbound Medicine ER -