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Evaluation of nanofibrous scaffolds obtained from blends of chitosan, gelatin and polycaprolactone for skin tissue engineering.
Int J Biol Macromol. 2017 Sep; 102:1174-1185.IJ

Abstract

Polymer blending is a strategy commonly used to obtain hybrid materials possessing properties better than those of the individual constituents regarding their use in scaffolds for Tissue Engineering. In the present work, the scaffolds produced by electrospinning solutions of polymeric blends obtained using a polyester (polycaprolactone, PCL), a polysaccharide (chitosan, CS) and a protein (gelatin extracted from cold water fish skin, GEL), were investigated. Solutions conductivity, shear viscosity and surface tension were determined. GEL-containing scaffolds were crosslinked with vapour phase glutaraldehyde (GTA). The scaffolds were characterized physico-chemically regarding fibre morphology, porosity, water contact angle, mechanical properties, chemical bonds and fibre and dimensional stability upon immersion in water and cell culture medium. The scaffolds were further tested in vitro for cell adhesion, growth and morphology of human foetal fibroblasts (cell line HFFF2). Results show that the nanofibrous scaffolds are hydrophilic and display the typical porosity of non-woven fibre mats. The CS/PCL and CS/PCL/GEL scaffolds have the highest elastic modulus (48MPa). Dimensional stability is best for the CS/PCL/GEL scaffolds. FTIR spectra confirm the occurrence of cross-linking reactions of GTA with both GEL and CS. Cell adhesion ratio ranked from excellent (close to 100%) to satisfactory (around 50%) in the order PCL/GEL>CS/GEL>CS/PCL/GEL>CS/PCL. Cell populations show an extended lag phase in comparison with the controls but cell proliferation occurs on all scaffolds until confluence is reached. In conclusion, all scaffolds studied possess characteristics that enable them to be used in skin tissue engineering but the CS/PCL/GEL scaffolds have better physical properties whereas the PCL/GEL scaffolds support a higher cell adhesion.

Authors+Show Affiliations

Departamento de Física, Faculdade de Ciências e Tecnologia da Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.Centro de Ecologia, Evolução e Alterações Ambientais/Departamento de Biologia Animal Faculdade de Ciências da Universidade de Lisboa, Campo Grande, 1749-016 Lisboa, Portugal.Centro de Ecologia, Evolução e Alterações Ambientais/Departamento de Biologia Animal Faculdade de Ciências da Universidade de Lisboa, Campo Grande, 1749-016 Lisboa, Portugal; Instituto Gulbenkian de Ciência, R. da Quinta Grande, 6, 2780-156 Oeiras, Portugal.Departamento de Física, Faculdade de Ciências e Tecnologia da Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.Departamento de Física, Faculdade de Ciências e Tecnologia da Universidade Nova de Lisboa, 2829-516 Caparica, Portugal. Electronic address: jcs@fct.unl.pt.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28487195

Citation

Gomes, Susana, et al. "Evaluation of Nanofibrous Scaffolds Obtained From Blends of Chitosan, Gelatin and Polycaprolactone for Skin Tissue Engineering." International Journal of Biological Macromolecules, vol. 102, 2017, pp. 1174-1185.
Gomes S, Rodrigues G, Martins G, et al. Evaluation of nanofibrous scaffolds obtained from blends of chitosan, gelatin and polycaprolactone for skin tissue engineering. Int J Biol Macromol. 2017;102:1174-1185.
Gomes, S., Rodrigues, G., Martins, G., Henriques, C., & Silva, J. C. (2017). Evaluation of nanofibrous scaffolds obtained from blends of chitosan, gelatin and polycaprolactone for skin tissue engineering. International Journal of Biological Macromolecules, 102, 1174-1185. https://doi.org/10.1016/j.ijbiomac.2017.05.004
Gomes S, et al. Evaluation of Nanofibrous Scaffolds Obtained From Blends of Chitosan, Gelatin and Polycaprolactone for Skin Tissue Engineering. Int J Biol Macromol. 2017;102:1174-1185. PubMed PMID: 28487195.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of nanofibrous scaffolds obtained from blends of chitosan, gelatin and polycaprolactone for skin tissue engineering. AU - Gomes,Susana, AU - Rodrigues,Gabriela, AU - Martins,Gabriel, AU - Henriques,Célia, AU - Silva,Jorge Carvalho, Y1 - 2017/05/06/ PY - 2017/01/27/received PY - 2017/04/28/revised PY - 2017/05/01/accepted PY - 2017/5/11/pubmed PY - 2018/3/7/medline PY - 2017/5/11/entrez KW - Blends KW - Chitosan KW - Electrospinning KW - Gelatin KW - Polycaprolactone KW - Skin substitutes SP - 1174 EP - 1185 JF - International journal of biological macromolecules JO - Int. J. Biol. Macromol. VL - 102 N2 - Polymer blending is a strategy commonly used to obtain hybrid materials possessing properties better than those of the individual constituents regarding their use in scaffolds for Tissue Engineering. In the present work, the scaffolds produced by electrospinning solutions of polymeric blends obtained using a polyester (polycaprolactone, PCL), a polysaccharide (chitosan, CS) and a protein (gelatin extracted from cold water fish skin, GEL), were investigated. Solutions conductivity, shear viscosity and surface tension were determined. GEL-containing scaffolds were crosslinked with vapour phase glutaraldehyde (GTA). The scaffolds were characterized physico-chemically regarding fibre morphology, porosity, water contact angle, mechanical properties, chemical bonds and fibre and dimensional stability upon immersion in water and cell culture medium. The scaffolds were further tested in vitro for cell adhesion, growth and morphology of human foetal fibroblasts (cell line HFFF2). Results show that the nanofibrous scaffolds are hydrophilic and display the typical porosity of non-woven fibre mats. The CS/PCL and CS/PCL/GEL scaffolds have the highest elastic modulus (48MPa). Dimensional stability is best for the CS/PCL/GEL scaffolds. FTIR spectra confirm the occurrence of cross-linking reactions of GTA with both GEL and CS. Cell adhesion ratio ranked from excellent (close to 100%) to satisfactory (around 50%) in the order PCL/GEL>CS/GEL>CS/PCL/GEL>CS/PCL. Cell populations show an extended lag phase in comparison with the controls but cell proliferation occurs on all scaffolds until confluence is reached. In conclusion, all scaffolds studied possess characteristics that enable them to be used in skin tissue engineering but the CS/PCL/GEL scaffolds have better physical properties whereas the PCL/GEL scaffolds support a higher cell adhesion. SN - 1879-0003 UR - https://www.unboundmedicine.com/medline/citation/28487195/Evaluation_of_nanofibrous_scaffolds_obtained_from_blends_of_chitosan_gelatin_and_polycaprolactone_for_skin_tissue_engineering_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0141-8130(17)30304-5 DB - PRIME DP - Unbound Medicine ER -