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ANTIOXIDANT ACTIVITY AND FT-IR ANALYSIS OF DATURA INNOXIA AND DATURA METEL LEAF AND SEED METHANOLIC EXTRACTS.

Abstract

BACKGROUND

Datura (family- Solanaceae), has a long history of being used as herbal medicine. These medicinal effects have been attributed to the phytochemicals present in the plant leaves and seeds, in particular alkaloids, flavonoids and phenolic compounds. The objective of this study was to investigate the methanolic leaf and seed extracts of Datura innoxia (DLP-I & DSP-I) and Datura metel (DLP-M & DSP-M) for their total phenolic, flavonoids and in-vitro antioxidant properties.

MATERIALS AND METHODS

Determination of total phenolic content and total flavonoid content and antioxidant activity in terms of total antioxidant assay, ABTS assay, DPPH assay and in-vitro lipid peroxidation inhibiting activity were determined along with the FT-IR (Fourier transform infrared spectroscopy) analysis of the extracts.

RESULTS

The highest total phenolic and total flavonoid content were registered by the D. innoxia leaf extract (70.26 ±1.12 mg GAE/g and 34.24 ± 1.28 mg RE/g respectively). Maximum DPPH radical scavenging activity was exerted by the leaf extract of D. innoxia (IC50 = 146.69 ± 8.46 μg/mL) among the four different methanolic extracts. The highest activity of the ABTS assay was found in Datura innoxia leaf extract (IC50 value = 149.42 ± 13.43 μg/mL) and the highest total antioxidant capacity was found to be present in D. innoxia leaf extract (221.25 ± 1.06 mg AAE/g) whereas D. metel seed extract registered the maximum lipid peroxidation inhibition activity (IC50 = 112 ± 1.30 μg/mL). The FT-IR data also supported the maximum activity in D. innoxia (leaf and seed) extracts.

CONCLUSION

The results thus obtained suggested that the plant Datura innoxia possess considerable antioxidant activity over Datura metel and therefore can be established as a potential source of natural antioxidant.

Authors+Show Affiliations

Department of Biochemistry, Panjab University, Chandigarh-160014, India.Department of Biochemistry, Panjab University, Chandigarh-160014, India.Department of Biochemistry, Panjab University, Chandigarh-160014, India.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28487888

Citation

Bhardwaj, Khushboo, et al. "ANTIOXIDANT ACTIVITY and FT-IR ANALYSIS of DATURA INNOXIA and DATURA METEL LEAF and SEED METHANOLIC EXTRACTS." African Journal of Traditional, Complementary, and Alternative Medicines : AJTCAM, vol. 13, no. 5, 2016, pp. 7-16.
Bhardwaj K, Kumar S, Ojha S. ANTIOXIDANT ACTIVITY AND FT-IR ANALYSIS OF DATURA INNOXIA AND DATURA METEL LEAF AND SEED METHANOLIC EXTRACTS. Afr J Tradit Complement Altern Med. 2016;13(5):7-16.
Bhardwaj, K., Kumar, S., & Ojha, S. (2016). ANTIOXIDANT ACTIVITY AND FT-IR ANALYSIS OF DATURA INNOXIA AND DATURA METEL LEAF AND SEED METHANOLIC EXTRACTS. African Journal of Traditional, Complementary, and Alternative Medicines : AJTCAM, 13(5), 7-16. https://doi.org/10.21010/ajtcam.v13i5.2
Bhardwaj K, Kumar S, Ojha S. ANTIOXIDANT ACTIVITY and FT-IR ANALYSIS of DATURA INNOXIA and DATURA METEL LEAF and SEED METHANOLIC EXTRACTS. Afr J Tradit Complement Altern Med. 2016;13(5):7-16. PubMed PMID: 28487888.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - ANTIOXIDANT ACTIVITY AND FT-IR ANALYSIS OF DATURA INNOXIA AND DATURA METEL LEAF AND SEED METHANOLIC EXTRACTS. AU - Bhardwaj,Khushboo, AU - Kumar,Sanjiv, AU - Ojha,Sudarshan, Y1 - 2016/08/12/ PY - 2017/5/11/entrez PY - 2017/5/11/pubmed PY - 2017/8/8/medline KW - ABTS KW - Antioxidants KW - DPPH KW - Datura species KW - FT-IR KW - Methanolic extract SP - 7 EP - 16 JF - African journal of traditional, complementary, and alternative medicines : AJTCAM JO - Afr J Tradit Complement Altern Med VL - 13 IS - 5 N2 - BACKGROUND: Datura (family- Solanaceae), has a long history of being used as herbal medicine. These medicinal effects have been attributed to the phytochemicals present in the plant leaves and seeds, in particular alkaloids, flavonoids and phenolic compounds. The objective of this study was to investigate the methanolic leaf and seed extracts of Datura innoxia (DLP-I & DSP-I) and Datura metel (DLP-M & DSP-M) for their total phenolic, flavonoids and in-vitro antioxidant properties. MATERIALS AND METHODS: Determination of total phenolic content and total flavonoid content and antioxidant activity in terms of total antioxidant assay, ABTS assay, DPPH assay and in-vitro lipid peroxidation inhibiting activity were determined along with the FT-IR (Fourier transform infrared spectroscopy) analysis of the extracts. RESULTS: The highest total phenolic and total flavonoid content were registered by the D. innoxia leaf extract (70.26 ±1.12 mg GAE/g and 34.24 ± 1.28 mg RE/g respectively). Maximum DPPH radical scavenging activity was exerted by the leaf extract of D. innoxia (IC50 = 146.69 ± 8.46 μg/mL) among the four different methanolic extracts. The highest activity of the ABTS assay was found in Datura innoxia leaf extract (IC50 value = 149.42 ± 13.43 μg/mL) and the highest total antioxidant capacity was found to be present in D. innoxia leaf extract (221.25 ± 1.06 mg AAE/g) whereas D. metel seed extract registered the maximum lipid peroxidation inhibition activity (IC50 = 112 ± 1.30 μg/mL). The FT-IR data also supported the maximum activity in D. innoxia (leaf and seed) extracts. CONCLUSION: The results thus obtained suggested that the plant Datura innoxia possess considerable antioxidant activity over Datura metel and therefore can be established as a potential source of natural antioxidant. SN - 2505-0044 UR - https://www.unboundmedicine.com/medline/citation/28487888/ANTIOXIDANT_ACTIVITY_AND_FT_IR_ANALYSIS_OF_DATURA_INNOXIA_AND_DATURA_METEL_LEAF_AND_SEED_METHANOLIC_EXTRACTS_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/28487888/ DB - PRIME DP - Unbound Medicine ER -