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Antiproliferative activity in tumor cell lines, antioxidant capacity and total phenolic, flavonoid and tannin contents of Myrciaria floribunda.
An Acad Bras Cienc 2017 Apr-Jun; 89(2):1111-1120AA

Abstract

Myrciaria floribunda (H. West ex Willd.) O. Berg, Myrtaceae, is a native plant species of the Atlantic Rain Forest, from north to south of Brazil. The lyophilized ethyl acetate extract from the leaves of M. floribunda was investigated for its antiproliferative activity in tumor cell lines, antioxidant capacity and its total phenolic, flavonoid and tannin contents. Antiproliferative activity was tested in vitro against seven human cancer cells and against immortalized human skin keratinocytes line (HaCat, no cancer cell). Antioxidant activity was determined using 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and oxygen radical absorbing capacity (ORAC) assays and total phenolic, flavonoid and tannin contents were determined by spectrophotometric techniques. Ethyl acetate extract of M. floribunda exhibited antiproliferative activity against cancer cell lines with total growth inhibition (TGI) between 69.70 and 172.10 µg/mL. For HaCat cell, TGI value was 213.60 µg/mL. M. floribunda showed a strong antioxidant potential: EC50 of 45.89±0.42 µg/mL and 0.55±0.05 mmol TE/g for DPPH and ORAC, respectively. Total phenolic content was 0.23±0.013g gallic acid equivalents (GAE)/g extract and exhibited 13.10±1.60% of tannins content. The content of flavonoid was 24.08±0.44% expressed as rutin equivalents. These results provide a direction for further researches about the antitumoral potential of M. floribunda.

Authors+Show Affiliations

Laboratório de Tecnologia de Produtos Naturais, Faculdade de Farmácia, Universidade Federal Fluminense, Rua Mário Viana, 523, Santa Rosa, 24241-000 Niterói, RJ, Brazil.Laboratório Multidisciplinar de Ciências Farmacêuticas, Faculdade de Farmácia, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Avenida Carlos Chagas Filho, 373, Ilha do Fundão, 21941-590 Rio de Janeiro, RJ, Brazil.Laboratório de Tecnologia de Produtos Naturais, Faculdade de Farmácia, Universidade Federal Fluminense, Rua Mário Viana, 523, Santa Rosa, 24241-000 Niterói, RJ, Brazil.Laboratório de Tecnologia de Produtos Naturais, Faculdade de Farmácia, Universidade Federal Fluminense, Rua Mário Viana, 523, Santa Rosa, 24241-000 Niterói, RJ, Brazil.Laboratório de Tecnologia de Produtos Naturais, Faculdade de Farmácia, Universidade Federal Fluminense, Rua Mário Viana, 523, Santa Rosa, 24241-000 Niterói, RJ, Brazil.Laboratório de Tecnologia de Produtos Naturais, Faculdade de Farmácia, Universidade Federal Fluminense, Rua Mário Viana, 523, Santa Rosa, 24241-000 Niterói, RJ, Brazil.Laboratório de Tecnologia de Produtos Naturais, Faculdade de Farmácia, Universidade Federal Fluminense, Rua Mário Viana, 523, Santa Rosa, 24241-000 Niterói, RJ, Brazil.Departamento de Ciências, Faculdade de Formação de Professores, Universidade Estadual do Rio de Janeiro, Rua Dr. Francisco Portela, 1470, Patronato, 24435-005 São Gonçalo, RJ, Brazil.Divisão de Farmacologia e Toxicologia, Centro Pluridisciplinar de Pesquisas Químicas, Biológicas e Agrícolas, Universidade de Campinas, Caixa Postal 6171, 13083-970 Campinas, SP, Brazil.Laboratório de Tecnologia de Produtos Naturais, Faculdade de Farmácia, Universidade Federal Fluminense, Rua Mário Viana, 523, Santa Rosa, 24241-000 Niterói, RJ, Brazil.

Pub Type(s)

Evaluation Studies
Journal Article

Language

eng

PubMed ID

28489194

Citation

Tietbohl, Luis A C., et al. "Antiproliferative Activity in Tumor Cell Lines, Antioxidant Capacity and Total Phenolic, Flavonoid and Tannin Contents of Myrciaria Floribunda." Anais Da Academia Brasileira De Ciencias, vol. 89, no. 2, 2017, pp. 1111-1120.
Tietbohl LAC, Oliveira AP, Esteves RS, et al. Antiproliferative activity in tumor cell lines, antioxidant capacity and total phenolic, flavonoid and tannin contents of Myrciaria floribunda. An Acad Bras Cienc. 2017;89(2):1111-1120.
Tietbohl, L. A. C., Oliveira, A. P., Esteves, R. S., Albuquerque, R. D. D. G., Folly, D., Machado, F. P., ... Rocha, L. (2017). Antiproliferative activity in tumor cell lines, antioxidant capacity and total phenolic, flavonoid and tannin contents of Myrciaria floribunda. Anais Da Academia Brasileira De Ciencias, 89(2), pp. 1111-1120. doi:10.1590/0001-3765201720160461.
Tietbohl LAC, et al. Antiproliferative Activity in Tumor Cell Lines, Antioxidant Capacity and Total Phenolic, Flavonoid and Tannin Contents of Myrciaria Floribunda. An Acad Bras Cienc. 2017;89(2):1111-1120. PubMed PMID: 28489194.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antiproliferative activity in tumor cell lines, antioxidant capacity and total phenolic, flavonoid and tannin contents of Myrciaria floribunda. AU - Tietbohl,Luis A C, AU - Oliveira,Adriana P, AU - Esteves,Ricardo S, AU - Albuquerque,Ricardo D D G, AU - Folly,Diogo, AU - Machado,Francisco P, AU - Corrêa,Arthur L, AU - Santos,Marcelo G, AU - Ruiz,Ana L G, AU - Rocha,Leandro, Y1 - 2017/05/02/ PY - 2016/10/06/received PY - 2017/01/06/accepted PY - 2017/5/11/pubmed PY - 2018/3/9/medline PY - 2017/5/11/entrez SP - 1111 EP - 1120 JF - Anais da Academia Brasileira de Ciencias JO - An. Acad. Bras. Cienc. VL - 89 IS - 2 N2 - Myrciaria floribunda (H. West ex Willd.) O. Berg, Myrtaceae, is a native plant species of the Atlantic Rain Forest, from north to south of Brazil. The lyophilized ethyl acetate extract from the leaves of M. floribunda was investigated for its antiproliferative activity in tumor cell lines, antioxidant capacity and its total phenolic, flavonoid and tannin contents. Antiproliferative activity was tested in vitro against seven human cancer cells and against immortalized human skin keratinocytes line (HaCat, no cancer cell). Antioxidant activity was determined using 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and oxygen radical absorbing capacity (ORAC) assays and total phenolic, flavonoid and tannin contents were determined by spectrophotometric techniques. Ethyl acetate extract of M. floribunda exhibited antiproliferative activity against cancer cell lines with total growth inhibition (TGI) between 69.70 and 172.10 µg/mL. For HaCat cell, TGI value was 213.60 µg/mL. M. floribunda showed a strong antioxidant potential: EC50 of 45.89±0.42 µg/mL and 0.55±0.05 mmol TE/g for DPPH and ORAC, respectively. Total phenolic content was 0.23±0.013g gallic acid equivalents (GAE)/g extract and exhibited 13.10±1.60% of tannins content. The content of flavonoid was 24.08±0.44% expressed as rutin equivalents. These results provide a direction for further researches about the antitumoral potential of M. floribunda. SN - 1678-2690 UR - https://www.unboundmedicine.com/medline/citation/28489194/Antiproliferative_activity_in_tumor_cell_lines_antioxidant_capacity_and_total_phenolic_flavonoid_and_tannin_contents_of_Myrciaria_floribunda_ L2 - http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017005008103&lng=en&nrm=iso&tlng=en DB - PRIME DP - Unbound Medicine ER -