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Proliferative and antioxidant activity of Symphytum officinale root extract.
Nat Prod Res 2018; 32(5):605-609NP

Abstract

The root of Symphytum officinale L. is commonly used in folk medicine to promote the wound healing, reduce the inflammation and in the treatment of broken bones. The objective of our investigation was to analyse the extract from S. officinale in term of its antioxidant activity and the effect on cell viability and proliferation of human skin fibroblast (HSF). Moreover, the quantification of main phenolics and allantoin was conducted using HPLC-DAD method. Five compounds were found: rosmarinic, p-hydroxybenzoic, caffeic, chlorogenic and p-coumaric acid. DPPH, FRAP and TPC assay showed the high antioxidant activity of the extract. MTT test proved the stimulatory effect on cell metabolism and viability of HSF cells. Moreover, no changes in cytoskeleton structure and cells shape were observed. The obtained results indicate that non-toxic extract from S. officinale root has strong antioxidant potential and a beneficial effect on human skin fibroblasts.

Authors+Show Affiliations

a Department of Analytical Chemistry , Medical University of Lublin , Lublin , Poland.b Faculty of Biology and Biotechnology, Department of Virology and Immunology , Maria Curie-Sklodowska University , Lublin , Poland. c Department of General Ophthalmology , Medical University of Lublin , Lublin , Poland.a Department of Analytical Chemistry , Medical University of Lublin , Lublin , Poland.d Department of Pharmaceutical Biology , Wroclaw Medical University , Wroclaw , Poland.a Department of Analytical Chemistry , Medical University of Lublin , Lublin , Poland.a Department of Analytical Chemistry , Medical University of Lublin , Lublin , Poland.a Department of Analytical Chemistry , Medical University of Lublin , Lublin , Poland.a Department of Analytical Chemistry , Medical University of Lublin , Lublin , Poland.d Department of Pharmaceutical Biology , Wroclaw Medical University , Wroclaw , Poland.e Children's Orthopedics Department , Medical University of Lublin , Lublin , Poland.a Department of Analytical Chemistry , Medical University of Lublin , Lublin , Poland.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28490191

Citation

Sowa, Ireneusz, et al. "Proliferative and Antioxidant Activity of Symphytum Officinale Root Extract." Natural Product Research, vol. 32, no. 5, 2018, pp. 605-609.
Sowa I, Paduch R, Strzemski M, et al. Proliferative and antioxidant activity of Symphytum officinale root extract. Nat Prod Res. 2018;32(5):605-609.
Sowa, I., Paduch, R., Strzemski, M., Zielińska, S., Rydzik-Strzemska, E., Sawicki, J., ... Wójciak-Kosior, M. (2018). Proliferative and antioxidant activity of Symphytum officinale root extract. Natural Product Research, 32(5), pp. 605-609. doi:10.1080/14786419.2017.1326492.
Sowa I, et al. Proliferative and Antioxidant Activity of Symphytum Officinale Root Extract. Nat Prod Res. 2018;32(5):605-609. PubMed PMID: 28490191.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Proliferative and antioxidant activity of Symphytum officinale root extract. AU - Sowa,Ireneusz, AU - Paduch,Roman, AU - Strzemski,Maciej, AU - Zielińska,Sylwia, AU - Rydzik-Strzemska,Ewelina, AU - Sawicki,Jan, AU - Kocjan,Ryszard, AU - Polkowski,Janusz, AU - Matkowski,Adam, AU - Latalski,Michał, AU - Wójciak-Kosior,Magdalena, Y1 - 2017/05/10/ PY - 2017/5/12/pubmed PY - 2018/3/27/medline PY - 2017/5/12/entrez KW - Symphytum officinale KW - allantoin KW - antioxidant activity KW - cell viability KW - comfrey KW - human skin fibroblast KW - phenolic acids SP - 605 EP - 609 JF - Natural product research JO - Nat. Prod. Res. VL - 32 IS - 5 N2 - The root of Symphytum officinale L. is commonly used in folk medicine to promote the wound healing, reduce the inflammation and in the treatment of broken bones. The objective of our investigation was to analyse the extract from S. officinale in term of its antioxidant activity and the effect on cell viability and proliferation of human skin fibroblast (HSF). Moreover, the quantification of main phenolics and allantoin was conducted using HPLC-DAD method. Five compounds were found: rosmarinic, p-hydroxybenzoic, caffeic, chlorogenic and p-coumaric acid. DPPH, FRAP and TPC assay showed the high antioxidant activity of the extract. MTT test proved the stimulatory effect on cell metabolism and viability of HSF cells. Moreover, no changes in cytoskeleton structure and cells shape were observed. The obtained results indicate that non-toxic extract from S. officinale root has strong antioxidant potential and a beneficial effect on human skin fibroblasts. SN - 1478-6427 UR - https://www.unboundmedicine.com/medline/citation/28490191/Proliferative_and_antioxidant_activity_of_Symphytum_officinale_root_extract_ L2 - http://www.tandfonline.com/doi/full/10.1080/14786419.2017.1326492 DB - PRIME DP - Unbound Medicine ER -