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Evaluation of dermal wound healing activity and in vitro antibacterial and antioxidant activities of a new exopolysaccharide produced by Lactobacillus sp.Ca6.
Int J Biol Macromol. 2017 Oct; 103:194-201.IJ

Abstract

The present study aimed to evaluate the in vitro antibacterial and antioxidant activities and the in vivo wound healing performance of a noval exopolysaccharides (EPS-Ca6) produced by Lactobacillus sp.Ca6 strain. The results showed that EPS-Ca6 had a potential antioxidant activity determined through four different assays: DPPH scavenging activity, reducing power, β-carotene bleaching by linoleic acid assay, and Metal chelating activities. It also exhibited significant antibacterial activity against pathogenic bacteria Salmonella enterica and Micrococcus luteus. The wound healing activity of the EPS-Ca6, using excision wound model in rats, showed that this novel EPS accelerated significantly wound healing activity as compared to the control group, and a total closure was achieved after 14days of wound induction. Furthermore, histological examination of biopsies showed fully re-epithelialized wound with a complete epidermal regeneration. Overall the finding indicates that the EPS-Ca6 might be useful as a wound healing agent in modern medicine.

Authors+Show Affiliations

Laboratoire de Microorganismes et de Biomolécules (LMB), Centre de Biotechnologie de Sfax, Route de Sidi Mansour Km 6, BP "1177" 3018 Sfax-Tunisia.Laboratoire de Génie Enzymatique et de Microbiologie, Université de Sfax, Ecole Nationale d'Ingénieurs de Sfax, B.P. 1173-3038 Sfax, Tunisia.Laboratoire de Microorganismes et de Biomolécules (LMB), Centre de Biotechnologie de Sfax, Route de Sidi Mansour Km 6, BP "1177" 3018 Sfax-Tunisia.Laboratoire de Microorganismes et de Biomolécules (LMB), Centre de Biotechnologie de Sfax, Route de Sidi Mansour Km 6, BP "1177" 3018 Sfax-Tunisia.Laboratoire de Pharmacologie, Faculté de Médecine à Sfax, Route Majida Boulila, 3028 Sfax, Tunisia.Laboratoire d'Anatomie Pathologique, CHU Habib Bourguiba, Sfax, Tunisia.Laboratoire de Microorganismes et de Biomolécules (LMB), Centre de Biotechnologie de Sfax, Route de Sidi Mansour Km 6, BP "1177" 3018 Sfax-Tunisia. Electronic address: riadh_fss@yahoo.fr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28495632

Citation

Trabelsi, Imen, et al. "Evaluation of Dermal Wound Healing Activity and in Vitro Antibacterial and Antioxidant Activities of a New Exopolysaccharide Produced By Lactobacillus Sp.Ca6." International Journal of Biological Macromolecules, vol. 103, 2017, pp. 194-201.
Trabelsi I, Ktari N, Ben Slima S, et al. Evaluation of dermal wound healing activity and in vitro antibacterial and antioxidant activities of a new exopolysaccharide produced by Lactobacillus sp.Ca6. Int J Biol Macromol. 2017;103:194-201.
Trabelsi, I., Ktari, N., Ben Slima, S., Triki, M., Bardaa, S., Mnif, H., & Ben Salah, R. (2017). Evaluation of dermal wound healing activity and in vitro antibacterial and antioxidant activities of a new exopolysaccharide produced by Lactobacillus sp.Ca6. International Journal of Biological Macromolecules, 103, 194-201. https://doi.org/10.1016/j.ijbiomac.2017.05.017
Trabelsi I, et al. Evaluation of Dermal Wound Healing Activity and in Vitro Antibacterial and Antioxidant Activities of a New Exopolysaccharide Produced By Lactobacillus Sp.Ca6. Int J Biol Macromol. 2017;103:194-201. PubMed PMID: 28495632.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of dermal wound healing activity and in vitro antibacterial and antioxidant activities of a new exopolysaccharide produced by Lactobacillus sp.Ca6. AU - Trabelsi,Imen, AU - Ktari,Naourez, AU - Ben Slima,Sirine, AU - Triki,Mehdi, AU - Bardaa,Sana, AU - Mnif,Hela, AU - Ben Salah,Riadh, Y1 - 2017/05/08/ PY - 2016/12/15/received PY - 2017/02/16/revised PY - 2017/05/05/accepted PY - 2017/5/13/pubmed PY - 2018/3/31/medline PY - 2017/5/13/entrez KW - Antibacterial activity KW - Antioxidant activity KW - EPS-Ca(6) KW - Wound healing SP - 194 EP - 201 JF - International journal of biological macromolecules JO - Int J Biol Macromol VL - 103 N2 - The present study aimed to evaluate the in vitro antibacterial and antioxidant activities and the in vivo wound healing performance of a noval exopolysaccharides (EPS-Ca6) produced by Lactobacillus sp.Ca6 strain. The results showed that EPS-Ca6 had a potential antioxidant activity determined through four different assays: DPPH scavenging activity, reducing power, β-carotene bleaching by linoleic acid assay, and Metal chelating activities. It also exhibited significant antibacterial activity against pathogenic bacteria Salmonella enterica and Micrococcus luteus. The wound healing activity of the EPS-Ca6, using excision wound model in rats, showed that this novel EPS accelerated significantly wound healing activity as compared to the control group, and a total closure was achieved after 14days of wound induction. Furthermore, histological examination of biopsies showed fully re-epithelialized wound with a complete epidermal regeneration. Overall the finding indicates that the EPS-Ca6 might be useful as a wound healing agent in modern medicine. SN - 1879-0003 UR - https://www.unboundmedicine.com/medline/citation/28495632/Evaluation_of_dermal_wound_healing_activity_and_in_vitro_antibacterial_and_antioxidant_activities_of_a_new_exopolysaccharide_produced_by_Lactobacillus_sp_Ca6_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0141-8130(16)32906-3 DB - PRIME DP - Unbound Medicine ER -