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Different Chromatographic Methods for Simultaneous Determination of Mefenamic Acid and Two of Its Toxic Impurities.
J Chromatogr Sci 2017; 55(7):766-772JC

Abstract

Two sensitive, accurate and precise chromatographic methods mentioned as TLC-densitometric method and RP-HPLC-DAD method, were developed and validated for the simultaneous determination of mefenamic acid (MEF) and its two toxic impurities, benzoic acid (BA) and 2,3-dimethylaniline (DMA). In the proposed TLC-densitometric method a developing system consisting of chloroform:acetone:acetic acid:ammonia solution(70:30:2:2, v/v/v/v) was used, TLC aluminum plates 60 F254 was used as a stationary phase and the separated bands were UV-scanned at 225 nm. While the proposed RP-HPLC-DAD method depended on chromatographic separation on C18 column using 0.05 M KH2PO4 buffer: acetonitrile (40:60, v/v) as a mobile phase at constant flow rate of 1 mL/min with UV detection at 225 nm. Linear relationships were obtained in the ranges of 0.3-2, 0.3-2 and 0.3-1.8 μg/band (for TLC-densitometric method) and in the ranges of 7-50, 10-50 and 7-50 μg/mL (for HPLC-DAD method) for MEF, BA and DMA, respectively. Factors affecting the developed methods have been studied and optimized. Moreover ,the proposed methods were successfully applied for determination of the studied drug in its pharmaceutical dosage form. The methods showed no significance difference when compared with the reported method using F-test and Student's-t test. The low of detection and quantization limits of the proposed methods get them suitable for quality control and stability studies of MEF in pharmaceutical formulation. The developed methods have advantages of being more selective and sensitive than the published methods.

Authors+Show Affiliations

Medicinal Chemistry Department, Faculty of Pharmacy, Nahda University, Beni-Suef, Egypt.Analytical Chemistry Department, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Egypt.Analytical Chemistry Department, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Egypt.Medicinal Chemistry Department, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Egypt.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28498911

Citation

Morcoss, Martha M., et al. "Different Chromatographic Methods for Simultaneous Determination of Mefenamic Acid and Two of Its Toxic Impurities." Journal of Chromatographic Science, vol. 55, no. 7, 2017, pp. 766-772.
Morcoss MM, Abdelwahab NS, Ali NW, et al. Different Chromatographic Methods for Simultaneous Determination of Mefenamic Acid and Two of Its Toxic Impurities. J Chromatogr Sci. 2017;55(7):766-772.
Morcoss, M. M., Abdelwahab, N. S., Ali, N. W., & Elsaady, M. T. (2017). Different Chromatographic Methods for Simultaneous Determination of Mefenamic Acid and Two of Its Toxic Impurities. Journal of Chromatographic Science, 55(7), pp. 766-772. doi:10.1093/chromsci/bmx034.
Morcoss MM, et al. Different Chromatographic Methods for Simultaneous Determination of Mefenamic Acid and Two of Its Toxic Impurities. J Chromatogr Sci. 2017 Aug 1;55(7):766-772. PubMed PMID: 28498911.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Different Chromatographic Methods for Simultaneous Determination of Mefenamic Acid and Two of Its Toxic Impurities. AU - Morcoss,Martha M, AU - Abdelwahab,Nada S, AU - Ali,Nouruddin W, AU - Elsaady,Mohammed T, PY - 2015/11/05/received PY - 2017/5/13/pubmed PY - 2018/3/6/medline PY - 2017/5/13/entrez SP - 766 EP - 772 JF - Journal of chromatographic science JO - J Chromatogr Sci VL - 55 IS - 7 N2 - Two sensitive, accurate and precise chromatographic methods mentioned as TLC-densitometric method and RP-HPLC-DAD method, were developed and validated for the simultaneous determination of mefenamic acid (MEF) and its two toxic impurities, benzoic acid (BA) and 2,3-dimethylaniline (DMA). In the proposed TLC-densitometric method a developing system consisting of chloroform:acetone:acetic acid:ammonia solution(70:30:2:2, v/v/v/v) was used, TLC aluminum plates 60 F254 was used as a stationary phase and the separated bands were UV-scanned at 225 nm. While the proposed RP-HPLC-DAD method depended on chromatographic separation on C18 column using 0.05 M KH2PO4 buffer: acetonitrile (40:60, v/v) as a mobile phase at constant flow rate of 1 mL/min with UV detection at 225 nm. Linear relationships were obtained in the ranges of 0.3-2, 0.3-2 and 0.3-1.8 μg/band (for TLC-densitometric method) and in the ranges of 7-50, 10-50 and 7-50 μg/mL (for HPLC-DAD method) for MEF, BA and DMA, respectively. Factors affecting the developed methods have been studied and optimized. Moreover ,the proposed methods were successfully applied for determination of the studied drug in its pharmaceutical dosage form. The methods showed no significance difference when compared with the reported method using F-test and Student's-t test. The low of detection and quantization limits of the proposed methods get them suitable for quality control and stability studies of MEF in pharmaceutical formulation. The developed methods have advantages of being more selective and sensitive than the published methods. SN - 1945-239X UR - https://www.unboundmedicine.com/medline/citation/28498911/Different_Chromatographic_Methods_for_Simultaneous_Determination_of_Mefenamic_Acid_and_Two_of_Its_Toxic_Impurities_ L2 - https://academic.oup.com/chromsci/article-lookup/doi/10.1093/chromsci/bmx034 DB - PRIME DP - Unbound Medicine ER -