Tags

Type your tag names separated by a space and hit enter

Matrine promotes oligodendrocyte development in CNS autoimmunity through the PI3K/Akt signaling pathway.
Life Sci 2017; 180:36-41LS

Abstract

AIMS

Matrine (MAT), a quinolizidine alkaloid derived from the herb Radix Sophorae flavescens, has been recently found to be beneficial in experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis, mainly through its anti-inflammatory effect. In the present study, we tested the effect of MAT on ongoing EAE and defined possible mechanisms underlying its effects on myelination and oligodendrocytes.

MAIN METHODS

EAE was induced in C57BL/6 mice and MAT treatment was started at disease onset. Clinical scores were monitored daily; spinal cords and the corpus callosum brain region of mice were harvested on day 23 p.i. for inflammatory infiltration and demyelination of the central nervous system. Myelin content and the development of oligodendrocytes and their precursors were determined by immunostaining, and expression of p-Akt, p-mTOR, p-PI3K, and p-P70S6 was determined by Western blot.

KEY FINDINGS

MAT effectively suppressed EAE severity and increased the expression of proteolipid protein, a myelin protein that is a marker of CNS myelin. MAT treatment largely increased the number of mature oligodendrocytes, and significantly activated the PI3K/Akt/mTOR signaling pathway, which is required for oligodendrocyte survival and axon myelination.

SIGNIFICANCE

These findings demonstrate a beneficial effect of MAT on oligodendrocyte differentiation and myelination during EAE, most likely through activating the PI3K/Akt/mTOR signaling pathway.

Authors+Show Affiliations

Department of Pharmacy, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, China.Department of Pharmacy, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, China.Department of Pharmacy, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, China.Chinese Medicine Research Institute of Henan Province, Zhengzhou 450004, Henan, China.Department of Pharmacy, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, China.Department of Neurology, Thomas Jefferson University, Philadelphia, PA 19107, USA.Department of Pharmacy, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, China. Electronic address: zhulin66zhulin@126.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28499934

Citation

Liu, Shu-Qing, et al. "Matrine Promotes Oligodendrocyte Development in CNS Autoimmunity Through the PI3K/Akt Signaling Pathway." Life Sciences, vol. 180, 2017, pp. 36-41.
Liu SQ, Zhang ML, Zhang HJ, et al. Matrine promotes oligodendrocyte development in CNS autoimmunity through the PI3K/Akt signaling pathway. Life Sci. 2017;180:36-41.
Liu, S. Q., Zhang, M. L., Zhang, H. J., Liu, F. Z., Chu, R. J., Zhang, G. X., & Zhu, L. (2017). Matrine promotes oligodendrocyte development in CNS autoimmunity through the PI3K/Akt signaling pathway. Life Sciences, 180, pp. 36-41. doi:10.1016/j.lfs.2017.05.010.
Liu SQ, et al. Matrine Promotes Oligodendrocyte Development in CNS Autoimmunity Through the PI3K/Akt Signaling Pathway. Life Sci. 2017 Jul 1;180:36-41. PubMed PMID: 28499934.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Matrine promotes oligodendrocyte development in CNS autoimmunity through the PI3K/Akt signaling pathway. AU - Liu,Shu-Qing, AU - Zhang,Ming-Liang, AU - Zhang,HUi-Jun, AU - Liu,Fang-Zhou, AU - Chu,Rao-Juan, AU - Zhang,Guang-Xian, AU - Zhu,Lin, Y1 - 2017/05/10/ PY - 2017/03/07/received PY - 2017/05/03/revised PY - 2017/05/09/accepted PY - 2017/5/14/pubmed PY - 2017/7/1/medline PY - 2017/5/14/entrez KW - Experimental autoimmune encephalomyelitis (EAE) KW - Matrine (MAT) KW - Multiple sclerosis (MS) KW - Myelination KW - Oligodendrocyte KW - PI3K/Akt/mTOR SP - 36 EP - 41 JF - Life sciences JO - Life Sci. VL - 180 N2 - AIMS: Matrine (MAT), a quinolizidine alkaloid derived from the herb Radix Sophorae flavescens, has been recently found to be beneficial in experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis, mainly through its anti-inflammatory effect. In the present study, we tested the effect of MAT on ongoing EAE and defined possible mechanisms underlying its effects on myelination and oligodendrocytes. MAIN METHODS: EAE was induced in C57BL/6 mice and MAT treatment was started at disease onset. Clinical scores were monitored daily; spinal cords and the corpus callosum brain region of mice were harvested on day 23 p.i. for inflammatory infiltration and demyelination of the central nervous system. Myelin content and the development of oligodendrocytes and their precursors were determined by immunostaining, and expression of p-Akt, p-mTOR, p-PI3K, and p-P70S6 was determined by Western blot. KEY FINDINGS: MAT effectively suppressed EAE severity and increased the expression of proteolipid protein, a myelin protein that is a marker of CNS myelin. MAT treatment largely increased the number of mature oligodendrocytes, and significantly activated the PI3K/Akt/mTOR signaling pathway, which is required for oligodendrocyte survival and axon myelination. SIGNIFICANCE: These findings demonstrate a beneficial effect of MAT on oligodendrocyte differentiation and myelination during EAE, most likely through activating the PI3K/Akt/mTOR signaling pathway. SN - 1879-0631 UR - https://www.unboundmedicine.com/medline/citation/28499934/Matrine_promotes_oligodendrocyte_development_in_CNS_autoimmunity_through_the_PI3K/Akt_signaling_pathway_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0024-3205(17)30230-8 DB - PRIME DP - Unbound Medicine ER -