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Using reduced graphene oxide-Ca:CdSe nanocomposite to enhance photoelectrochemical activity of gold nanoparticles functionalized tungsten oxide for highly sensitive prostate specific antigen detection.
Biosens Bioelectron. 2017 Oct 15; 96:239-245.BB

Abstract

An ultrasensitive sandwich-type photoelectrochemical (PEC) immunosensor was constructed for the detection of prostate specific antigen (PSA). In this work, Au-nanoparticle-loaded tungsten oxide (WO3-Au) hybrid composites was applied as PEC sensing platform, while Ca ions doped CdSe equipped on the conducting framework of reduced graphene oxide (rGO-Ca:CdSe) nanocomposites were employed as the signal amplification probe. As for WO3-Au, massive Au nanoparticles were formed on the surface of WO3 without any additional reducing agent, providing a novel nanocarriers for anchoring plenty of the primary antibodies due to the large specific surface area and good biocompatibility by chemical bonding between Au nanoparticles and -NH2 of antibodies. Besides, the incorporation of the rGO and the doping of Ca ions could improve the conductivity and hinder the recombination of electron-hole pairs of CdSe nanoparticles effectively, thereby enhancing the photocurrent conversion efficiency. Based on the sandwich immunoreaction, the primary antibody was immobilized onto WO3-Au substrate, after the formed rGO-Ca:CdSe labels were captured onto the electrode surface via the specific antibody-antigen interaction, the photocurrent intensity could be further enhanced due to the sensitization effect. Under the optimal conditions, the proposed PEC immunosensor shows a linear relationship between photocurrent variation and the logarithm of PSA concentration in the wide range of 5pgmL-1 to 50ngmL-1 with a low detection limit of 2.6pgmL-1 (S/N=3). Moreover, it also presented good stability and acceptable specificity, indicating the potential applications in clinical diagnostics.

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Authors+Show Affiliations

Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.

Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.

Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.

Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.

Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.

Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.

Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China. Electronic address: wudan791108@163.com.

Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.

MeSH

Antibodies, ImmobilizedBiosensing TechniquesCadmium CompoundsElectrochemical TechniquesGoldGraphiteHumansImmunoassayLimit of DetectionMetal NanoparticlesNanocompositesOxidation-ReductionOxidesPhotochemical ProcessesProstate-Specific AntigenSelenium CompoundsTungsten

Pub Type(s)

Evaluation Study

Journal Article

Language

eng

PubMed ID

28500948

Citation

Wang, Xueping, et al. "Using Reduced Graphene oxide-Ca:CdSe Nanocomposite to Enhance Photoelectrochemical Activity of Gold Nanoparticles Functionalized Tungsten Oxide for Highly Sensitive Prostate Specific Antigen Detection." Biosensors & Bioelectronics, vol. 96, 2017, pp. 239-245.

Wang X, Xu R, Sun X, et al. Using reduced graphene oxide-Ca:CdSe nanocomposite to enhance photoelectrochemical activity of gold nanoparticles functionalized tungsten oxide for highly sensitive prostate specific antigen detection. Biosens Bioelectron. 2017;96:239-245.

Wang, X., Xu, R., Sun, X., Wang, Y., Ren, X., Du, B., Wu, D., & Wei, Q. (2017). Using reduced graphene oxide-Ca:CdSe nanocomposite to enhance photoelectrochemical activity of gold nanoparticles functionalized tungsten oxide for highly sensitive prostate specific antigen detection. Biosensors & Bioelectronics, 96, 239-245. https://doi.org/10.1016/j.bios.2017.04.052

Wang X, et al. Using Reduced Graphene oxide-Ca:CdSe Nanocomposite to Enhance Photoelectrochemical Activity of Gold Nanoparticles Functionalized Tungsten Oxide for Highly Sensitive Prostate Specific Antigen Detection. Biosens Bioelectron. 2017 Oct 15;96:239-245. PubMed PMID: 28500948.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Using reduced graphene oxide-Ca:CdSe nanocomposite to enhance photoelectrochemical activity of gold nanoparticles functionalized tungsten oxide for highly sensitive prostate specific antigen detection. AU - Wang,Xueping, AU - Xu,Rui, AU - Sun,Xu, AU - Wang,Yaoguang, AU - Ren,Xiang, AU - Du,Bin, AU - Wu,Dan, AU - Wei,Qin, Y1 - 2017/05/10/ PY - 2017/02/18/received PY - 2017/04/09/revised PY - 2017/04/20/accepted PY - 2017/5/14/pubmed PY - 2018/3/13/medline PY - 2017/5/14/entrez KW - Photoelectrochemical immunosensor KW - Prostate specific antigen KW - RGO-Ca:CdSe KW - Sandwich-type KW - WO(3)-Au SP - 239 EP - 245 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 96 N2 - An ultrasensitive sandwich-type photoelectrochemical (PEC) immunosensor was constructed for the detection of prostate specific antigen (PSA). In this work, Au-nanoparticle-loaded tungsten oxide (WO3-Au) hybrid composites was applied as PEC sensing platform, while Ca ions doped CdSe equipped on the conducting framework of reduced graphene oxide (rGO-Ca:CdSe) nanocomposites were employed as the signal amplification probe. As for WO3-Au, massive Au nanoparticles were formed on the surface of WO3 without any additional reducing agent, providing a novel nanocarriers for anchoring plenty of the primary antibodies due to the large specific surface area and good biocompatibility by chemical bonding between Au nanoparticles and -NH2 of antibodies. Besides, the incorporation of the rGO and the doping of Ca ions could improve the conductivity and hinder the recombination of electron-hole pairs of CdSe nanoparticles effectively, thereby enhancing the photocurrent conversion efficiency. Based on the sandwich immunoreaction, the primary antibody was immobilized onto WO3-Au substrate, after the formed rGO-Ca:CdSe labels were captured onto the electrode surface via the specific antibody-antigen interaction, the photocurrent intensity could be further enhanced due to the sensitization effect. Under the optimal conditions, the proposed PEC immunosensor shows a linear relationship between photocurrent variation and the logarithm of PSA concentration in the wide range of 5pgmL-1 to 50ngmL-1 with a low detection limit of 2.6pgmL-1 (S/N=3). Moreover, it also presented good stability and acceptable specificity, indicating the potential applications in clinical diagnostics. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/28500948/Using_reduced_graphene_oxide_Ca:CdSe_nanocomposite_to_enhance_photoelectrochemical_activity_of_gold_nanoparticles_functionalized_tungsten_oxide_for_highly_sensitive_prostate_specific_antigen_detection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(17)30276-2 DB - PRIME DP - Unbound Medicine ER -